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Rabbit Recombinant Monoclonal PTPN12 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human samples.

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Images

Western blot - Anti-PTPN12 antibody [EPR28198-148] (AB315338), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (AB315338), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (AB315338), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (AB315338), expandable thumbnail
  • Western blot - Anti-PTPN12 antibody [EPR28198-148] (AB315338), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)ICC/IFIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed:18559503). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed:17329398, PubMed:27134172). Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (PubMed:27134172).

Alternative names

Tyrosine-protein phosphatase non-receptor type 12, PTP-PEST, Protein-tyrosine phosphatase G1, PTPG1, PTPN12

Recommended products

Rabbit Recombinant Monoclonal PTPN12 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Transfected cell lysate - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28198-148
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Protein Tyrosine Phosphatase Non-Receptor Type 12 (PTPN12) often referred to as PTP-PeST is an enzyme that plays a significant mechanical role within cells. PTPN12 is part of the protein tyrosine phosphatase (PTP) family which is responsible for removing phosphate groups from phosphotyrosine residues on proteins. This activity regulates many cellular processes including growth and differentiation. PTPN12 has a molecular mass of approximately 86 kDa. It is ubiquitously expressed in various tissues but shows higher expression in hematopoietic cells.

Biological function summary

As a non-receptor type phosphatase PTPN12 partners with several proteins to perform essential cellular functions. It interacts with multiple components in the cell participating in the assembly of protein complexes that manage cytoskeletal dynamics and cell movement. PTPN12 influences signal transduction pathways by interacting with adapter proteins such as p130Cas and Paxillin which are vital for cell adhesion and migration. It also plays a role in maintaining cellular homeostasis by controlling signaling cascades that prevent excessive cell proliferation.

Pathways

PTPN12 takes part in critical signal transduction networks most notably the Integrin signaling and RhoA pathways. In the Integrin pathway PTPN12 negatively regulates signaling by dephosphorylating focal adhesion-associated proteins. This regulation affects cell attachment to the extracellular matrix and cell motility. The interaction of PTPN12 with proteins such as Src and FAK (Focal Adhesion Kinase) illustrates its involvement in these processes. In the RhoA pathway PTPN12 influences cytoskeletal rearrangement and cell shape regulation linking to its role in cell migration.

Associated diseases and disorders

Disruptions in PTPN12 function associate with cancer progression such as breast cancer and inflammatory disorders. In breast cancer physiological studies indicate that PTPN12 acts as a tumor suppressor. Its loss or reduced activity may lead to unregulated cell proliferation and metastasis by failing to restrain pathways involving Src and FAK. In inflammatory disorders altered activities of PTPN12 can affect immune cell function contributing to conditions like rheumatoid arthritis. The enzyme's dysfunction can amplify inflammatory signals through faults in the regulation of immune cells linking PTPN12 to increased disease susceptibility.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 4 µg

    Lane 2: 293T cells transfected with a human PTPN12 expression vector containing a myc-His-tag®, whole cell lysate at 4 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 110-125 kDa, 36 kDa

    Exposure time: 10s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PTPN12 with ab315338 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human breast cancer (PMID: 23435421; PMID: 23044628). The section was incubated with ab315338 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PTPN12 with ab315338 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human astrocytoma (PMID: 29743287). The section was incubated with ab315338 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PTPN12 with ab315338 at 1/100 (5.21 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung (PMID: 25868976). The section was incubated with ab315338 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 110-125 kDa

    Exposure time: 180s

  • Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 26 seconds; Lane 3: 136 seconds.

    All lanes: Western blot - Anti-PTPN12 antibody [EPR28198-148] (ab315338) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: THP-1 transfected with siRNA specifically targeting PTPN12 whole cell lysate at 20 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 110-125 kDa, 36 kDa

  • Flow Cytometry (Intracellular) - Anti-PTPN12 antibody [EPR28198-148] (ab315338), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PTPN12 antibody [EPR28198-148] (ab315338)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling PTPN12 with ab315338 at 1/50 dilution (1 ug, Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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