AB204321

Anti-PTPN14 antibody

Name: Anti-PTPN14 antibody (ab204321) | Abcam
Brand: Abcam Limited
SKU: AB204321
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal PTPN14 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PTPN14 aa 700-800.

View Alternative Names

PEZ, PTPD2, PTPN14, Tyrosine-protein phosphatase non-receptor type 14, Protein-tyrosine phosphatase pez

1 Images

Supplier Data

Western blot - Anti-PTPN14 antibody (AB204321)

Lane 1:

Western blot - Anti-PTPN14 antibody (ab204321)

Lanes 2 - 3:

Western blot - Anti-PTPN14 antibody (ab204321) at 0.4 µg/mL

Lane 1:

Molecular weight ladder

Lane 2:

U-251 MG cell lysate

Lane 3:

MCF7 cell lysate

Predicted band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human PTPN14 aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

Q15678

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTPN14 also known as Protein Tyrosine Phosphatase Non-Receptor Type 14 or Pez is a protein with a mass of approximately 112 kDa. This protein functions as a non-receptor type of protein tyrosine phosphatase which actively removes phosphate groups from tyrosine residues on proteins. This action modulates the signaling pathways within the cell. The protein is expressed in several tissues including liver kidney and lung indicating diverse roles in various organ systems.

Biological function summary

This protein tyrosine phosphatase actively regulates processes related to cell growth development and adhesion. PTPN14 is particularly associated with cellular junctions since it interacts with components of the cell cytoskeleton. Although it does not belong specifically to a large complex its interactions with cytoskeletal proteins like F-actin suggest its role in maintaining cell structure and mobility. Importantly it can serve as a mediator influencing the stability and assembly of intercellular junctions.

Pathways

PTPN14 plays significant roles in the regulation of the Hippo signaling pathway and the Wnt signaling pathway. In the Hippo pathway it interacts with proteins such as YAP which are important for the control of cell proliferation and apoptosis. The interaction with this pathway points to its effect on organ size regulation and suppression of excessive cellular growth. In the Wnt signaling pathway PTPN14 modulates the pathway by affecting the expression of downstream target genes interacting potentially with proteins like β-catenin.

Disruptions in PTPN14 activity have links to lymphatic-related conditions and certain cancers. Mutations in PTPN14 contribute to conditions such as lymphedema which involves fluid accumulation due to faulty lymphatic drainage. Additionally its involvement with YAP in the Hippo pathway suggests a connection to tumorigenesis as misregulation can lead to uncontrolled cell proliferation. Understanding these interactions helps in designing strategies for therapeutic interventions in these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.

See full target information PTPN14

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 15:69 PubMed38460058

2024

DDOST is associated with tumor immunosuppressive microenvironment in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Mei,Liuliu Pan,Min Huang,Dandan Bao,Hui Gao,Danhan Wang

Cell death & disease 14:188 PubMed36898991

2023

PTPN14 promotes gastric cancer progression by PI3KA/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Bingxin Guan,Sen Liu,Haiting Liu,Lin Song,Guohao Zhang,Ruinan Zhao,Chengjun Zhou,Peng Gao

Open medicine (Warsaw, Poland) 17:280-291 PubMed35233464

2022

circ_AKT3 knockdown suppresses cisplatin resistance in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Shi,Fang Wang

Analytical cellular pathology (Amsterdam) 2021:9290372 PubMed34712552

2021

FAT1 and PTPN14 Regulate the Malignant Progression and Chemotherapy Resistance of Esophageal Cancer through the Hippo Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzhi Lu,Zhenxin Wang,Ling Zhou,Zhaoming Ma,Jianguo Zhang,Yan Wu,Yan Shao,Yunyun Yang

Yonsei medical journal 61:750-761 PubMed32882759

2020

Exosomal CircPRRX1 Enhances Doxorubicin Resistance in Gastric Cancer by Regulating MiR-3064-5p/PTPN14 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shumin Wang,Mei Ping,Bin Song,Yarong Guo,Yuanfei Li,Junmei Jia

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTPN14 antibody

Western blot - Anti-PTPN14 antibody (AB204321)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise