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AB326108

Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free

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Rabbit Recombinant Monoclonal PTPN14 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

View Alternative Names

PEZ, PTPD2, PTPN14, Tyrosine-protein phosphatase non-receptor type 14, Protein-tyrosine phosphatase pez

3 Images
Western blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)
  • WB

Lab

Western blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)

This data was developed using ab326098, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : 293T(PMID : 10934049)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) 1 : 10000 (124Kda).

All lanes:

Western blot - Anti-PTPN14 antibody [EPR30487-534] (<a href='/en-us/products/primary-antibodies/ptpn14-antibody-epr30487-534-ab326098'>ab326098</a>) at 1/1000 dilution

Lane 1:

DU 145 (human prostate carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 80 µg

Lane 2:

DU 145 transfected with siRNA specifically targeting PTPN14 whole cell lysate at 80 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 135 kDa,124 kDa

false

Exposure time: 135s

Western blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)
  • WB

Lab

Western blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)

This data was developed using ab326098, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : small intestine

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lane 1 : 26 seconds, lane 2 : 180 seconds

All lanes:

Western blot - Anti-PTPN14 antibody [EPR30487-534] (<a href='/en-us/products/primary-antibodies/ptpn14-antibody-epr30487-534-ab326098'>ab326098</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 80 µg

Lane 2:

Mouse small intestine tissue lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 135 kDa,36 kDa

false

Dot Blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)
  • Dot

Lab

Dot Blot - Anti-PTPN14 antibody [EPR30487-534] - BSA and Azide free (AB326108)

This data was developed using ab326098, the same antibody clone in a different buffer formulation.

Dot blot analysis of PTPN14 using ab326098 at 1 : 1000 (0.515 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : His-tagged human PTPN14 recombinant protein fragment

Lane 2 : LIF-tagged human PTPN21 recombinant protein fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PTPN21.

All lanes:

Dot Blot - Anti-PTPN14 antibody [EPR30487-534] (<a href='/en-us/products/primary-antibodies/ptpn14-antibody-epr30487-534-ab326098'>ab326098</a>) at 1/1000 dilution

Lane 1:

His-tagged human PTPN14 recombinant protein fragment

Lane 2:

LIF-tagged human PTPN21 recombinant protein fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30487-534

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab326108 is the carrier-free version of ab326098

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine phosphatase which may play a role in the regulation of lymphangiogenesis, cell-cell adhesion, cell-matrix adhesion, cell migration, cell growth and also regulates TGF-beta gene expression, thereby modulating epithelial-mesenchymal transition. Mediates beta-catenin dephosphorylation at adhesion junctions. Acts as a negative regulator of the oncogenic property of YAP, a downstream target of the hippo pathway, in a cell density-dependent manner. May function as a tumor suppressor.
See full target information PTPN14
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com