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AB169040

Anti-PTPN22 antibody

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(1 Publication)

Mouse Polyclonal PTPN22 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PTPN22.

View Alternative Names

PTPN8, PTPN22, Tyrosine-protein phosphatase non-receptor type 22, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, Lymphoid phosphatase, PEST-domain phosphatase, LyP, PEP

1 Images
Western blot - Anti-PTPN22 antibody (AB169040)
  • WB

Unknown

Western blot - Anti-PTPN22 antibody (AB169040)

All lanes:

Western blot - Anti-PTPN22 antibody (ab169040) at 1 µg/mL

Lane 1:

PTPN22 transfected 293T cell lysate at 15 µL

Lane 2:

non transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

Predicted band size: 92 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PTPN22.

Q9Y2R2

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Complementary Products

Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
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Proteins & Peptides

AB132055

Recombinant Human PTPN22 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human PTPN22 protein (GST tag N-Terminus) (AB132055)

  • 0
  • 0
Primary Antibodies

AB111207

Anti-PTPRM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRM antibody (AB111207)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein tyrosine phosphatase non-receptor type 22 (PTPN22) also known as Lyp is a phosphatase enzyme with a molecular mass of approximately 98 kDa. It removes phosphate groups from specific proteins participating in the regulation of signaling pathways that control cellular processes. PTPN22 is expressed in lymphoid tissues including T-cells B-cells and other immune cells. This distribution indicates its role in immune system function and signaling.
Biological function summary

PTPN22 plays a role in modulating immune responses impacting the activation of T-cells and B-cells. By altering signal transduction pathways it affects the threshold for immune cell activation. PTPN22 interacts with other signaling molecules but it is not known to be a part of any well-described protein complex. Its activity influences immune tolerance and autoimmunity making it an important player in maintaining immune system balance.

Pathways

PTPN22 is involved in the T-cell receptor signaling and negative regulation pathways. It dephosphorylates lymphocyte-specific protein tyrosine kinase (LCK) and ZAP-70 which are pivotal in T-cell activation. By regulating such proteins PTPN22 plays a direct role in modulating T-cell responses. The signaling balance achieved through its actions prevents overactive immune responses that could lead to autoimmune conditions.

PTPN22 has strong connections to autoimmune diseases such as rheumatoid arthritis and type 1 diabetes. Variants in the PTPN22 gene particularly the R620W allele are associated with an increased risk for these conditions. This variant affects the interaction of PTPN22 with C-SRC tyrosine kinase altering the normal immune signaling and contributing to the development of autoimmunity. Understanding the role of PTPN22 in these diseases offers potential targets for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules (PubMed : 16461343, PubMed : 18056643). Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue (PubMed : 21719704). Dephosphorylates ZAP70 at its activating 'Tyr-493' residue (PubMed : 16461343). Dephosphorylates the immune system activator SKAP2 (PubMed : 21719704). Positively regulates toll-like receptor (TLR)-induced type 1 interferon production (PubMed : 23871208). Promotes host antiviral responses mediated by type 1 interferon (By similarity). Regulates NOD2-induced pro-inflammatory cytokine secretion and autophagy (PubMed : 23991106). Acts as an activator of NLRP3 inflammasome assembly by mediating dephosphorylation of 'Tyr-861' of NLRP3 (PubMed : 27043286). Dephosphorylates phospho-anandamide (p-AEA), an endocannabinoid to anandamide (also called N-arachidonoylethanolamide) (By similarity).
See full target information PTPN22
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:3105 PubMed31308371

2019

Apoptotic signalling targets the post-endocytic sorting machinery of the death receptor Fas/CD95.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Sharma,Antonio Carmona,Agnieszka Skowronek,Fangyan Yu,Mark O Collins,Sindhu Naik,Claire M Murzeau,Pei-Li Tseng,Kai S Erdmann
View all publications
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Product promise

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