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AB248184

Anti-PTPRE antibody [EPR6715] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PTPRE antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

Receptor-type tyrosine-protein phosphatase epsilon, Protein-tyrosine phosphatase epsilon, R-PTP-epsilon, PTPRE

3 Images
Flow Cytometry (Intracellular) - Anti-PTPRE antibody [EPR6715] - BSA and Azide free (AB248184)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PTPRE antibody [EPR6715] - BSA and Azide free (AB248184)

This data was developed using ab126788, the same antibody clone in a different buffer formulation.

ab126788 at 1/10 dilution staining PTPRE in permeabilized HL60 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).

Western blot - Anti-PTPRE antibody [EPR6715] - BSA and Azide free (AB248184)
  • WB

Unknown

Western blot - Anti-PTPRE antibody [EPR6715] - BSA and Azide free (AB248184)

This data was developed using ab126788, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PTPRE antibody [EPR6715] (<a href='/en-us/products/primary-antibodies/ptpre-antibody-epr6715-ab126788'>ab126788</a>) at 1/1000 dilution

Lane 1:

HL60 cell lysate, treated with TPA at 10 µg

Lane 2:

HL60 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

C2C12 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 81 kDa

false

OI-RD Scanning - Anti-PTPRE antibody [EPR6715] - BSA and Azide free (AB248184)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PTPRE antibody [EPR6715] - BSA and Azide free (AB248184)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6715

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248184 is the carrier-free version of ab126788.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTPRE also known as Protein Tyrosine Phosphatase Receptor Type E or PTPε acts as a tyrosine phosphatase that removes phosphate groups from specific tyrosine residues on proteins. This enzyme which has a molecular mass of approximately 83 kDa functions as a receptor-type phosphatase with roles in cell signaling. PTPRE is expressed across various tissues including high levels in the brain and testis suggesting diverse functional roles in different cellular contexts.
Biological function summary

Protein Tyrosine Phosphatase Receptor Type E regulates cellular processes such as growth differentiation and motility by modulating the phosphorylation state of key signaling components. PTPRE is not known as part of a specific protein complex but interacts with various proteins through its enzymatic activity. It maintains balance in cellular signal transduction processes by counterbalancing kinases which add phosphate groups. This regulatory role indicates its involvement in many critical cellular activities and responses.

Pathways

Protein Tyrosine Phosphatase Receptor Type E influences the MAPK/ERK pathway which plays an important role in cell division and the JAK/STAT signaling pathway important for immune response. In these pathways PTPRE interacts and regulates proteins such as ERK and STAT respectively. By modulating phosphorylation PTPRE influences pathway signaling outcomes and cellular responses to external stimuli showing its integrative role in controlling signal transduction dynamics.

Protein Tyrosine Phosphatase Receptor Type E has been associated with cancer and autoimmune diseases. PTPRE's activity in dephosphorylating targets like ERK and STAT can impact cell proliferation and immune regulation linking it to tumor growth and immune dysregulation. In some cancers abnormal PTPRE activity interacts with other signaling proteins such as Src-family kinases influencing oncogenic processes. This association makes PTPRE a protein of interest for understanding and potentially targeting disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1 plays a critical role in signaling transduction pathways and phosphoprotein network topology in red blood cells. May play a role in osteoclast formation and function (By similarity).. Isoform 2 acts as a negative regulator of insulin receptor (IR) signaling in skeletal muscle. Regulates insulin-induced tyrosine phosphorylation of insulin receptor (IR) and insulin receptor substrate 1 (IRS-1), phosphorylation of protein kinase B and glycogen synthase kinase-3 and insulin induced stimulation of glucose uptake (By similarity).. Isoform 1 and isoform 2 act as a negative regulator of FceRI-mediated signal transduction leading to cytokine production and degranulation, most likely by acting at the level of SYK to affect downstream events such as phosphorylation of SLP76 and LAT and mobilization of Ca(2+).
See full target information PTPRE
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1025 PubMed39164573

2024

CD58 defines regulatory macrophages within the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wu,Xiaoni Zhan,Meixi Jiang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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