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AB231723

Anti-PTPRH antibody

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Rabbit Polyclonal PTPRH antibody. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human, Pig samples. Immunogen corresponding to Recombinant Fragment Protein within Human PTPRH aa 800-1100.

View Alternative Names

SAP1, PTPRH, Receptor-type tyrosine-protein phosphatase H, R-PTP-H, Stomach cancer-associated protein tyrosine phosphatase 1, Transmembrane-type protein-tyrosine phosphatase type H, SAP-1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRH antibody (AB231723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRH antibody (AB231723)

Formalin-fixed, paraffin-embedded human liver tissue stained for PTPRH using ab231723 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRH antibody (AB231723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRH antibody (AB231723)

Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for PTPRH using ab231723 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRH antibody (AB231723)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTPRH antibody (AB231723)

Formalin-fixed, paraffin-embedded human skin cancer tissue stained for PTPRH using ab231723 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-PTPRH antibody (AB231723)
  • WB

Supplier Data

Western blot - Anti-PTPRH antibody (AB231723)

All lanes:

Western blot - Anti-PTPRH antibody (ab231723) at 3 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

Pig brain lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 122 kDa

false

Western blot - Anti-PTPRH antibody (AB231723)
  • WB

Supplier Data

Western blot - Anti-PTPRH antibody (AB231723)

All lanes:

Western blot - Anti-PTPRH antibody (ab231723) at 3 µg/mL

All lanes:

Recombinant human PTPRH protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 122 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PTPRH aa 800-1100. The exact immunogen used to generate this antibody is proprietary information.

Q9HD43

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTPRH also known as human protein tyrosine phosphatase receptor type H (SAP-1) is a receptor-type protein tyrosine phosphatase. It weighs around 130 kDa. This protein is mainly found in epithelial tissues including the liver pancreas stomach and intestines. PTPRH contains an extracellular domain a single transmembrane region and an intracellular catalytic domain playing a significant role in dephosphorylation processes.
Biological function summary

PTPRH functions as a regulator of cellular signaling processes by modulating phosphorylation levels. It acts in the cellular communication network impacting cell growth differentiation and adhesion. This protein does not usually exist as part of a larger complex but interacts directly with phosphorylated substrates to exert its activity. PTPRH’s role in modulating signal transduction affects various cellular processes from metabolism to immune responses.

Pathways

PTPRH shows involvement in the MAPK/ERK signaling pathway and the PI3K/AKT signaling pathway. These pathways are important for transmitting extracellular signals to regulate gene expression and cell survival. PTPRH impacts molecular partners such as ERK and AKT through dephosphorylation influencing cellular responses. Its activity also integrates with other signaling molecules ensuring accurate control over cellular functions.

PTPRH associates with several pathologies with notable implications in cancer and liver disease. Loss of PTPRH function or alterations in its expression levels have been linked to colorectal cancer development due to disrupted cellular signaling. Additionally changes in PTPRH activity can affect proteins like EGFR which plays a part in cell proliferation and survival further connecting it to oncogenic processes. Understanding PTPRH's role provides insights into mechanisms leading to disease progression and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase that may contribute to contact inhibition of cell growth and motility by mediating the dephosphorylation of focal adhesion-associated substrates and thus negatively regulating integrin-promoted signaling processes. Induces apoptotic cell death by at least two distinct mechanisms : inhibition of cell survival signaling mediated by PI 3-kinase, Akt, and ILK and activation of a caspase-dependent proapoptotic pathway. Inhibits the basal activity of LCK and its activation in response to TCR stimulation and TCR-induced activation of MAP kinase and surface expression of CD69. Inhibits TCR-induced tyrosine phosphorylation of LAT and ZAP70. Inhibits both basal activity of DOK1 and its CD2-induced tyrosine phosphorylation. Induces dephosphorylation of BCAR1, focal adhesion kinase and SRC. Reduces migratory activity of activity of Jurkat cells. Reduces tyrosine phosphorylation of CEACAM20 and thereby contributes to suppress the intestinal immune response CEACAM20 (By similarity).
See full target information PTPRH
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