AB13225

Anti-PTPRK antibody

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(2 Publications)

Rabbit Polyclonal PTPRK antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications.

View Alternative Names

PTPK, PTPRK, Receptor-type tyrosine-protein phosphatase kappa, Protein-tyrosine phosphatase kappa, R-PTP-kappa

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Western blot - Anti-PTPRK antibody (AB13225)

Recombinant PTP phosphatase domains (0.1μg of protein) were probed with ab13225 at 1/1000. The antibody recognises the recombinant PTPRK fusion protein at ~65 kDa. Ladder sizes are in kilodaltons.

Lane # Recombinant Protein

1 GLEPP1 (PTP RO)
2 SAP 1 (PTP RH)
3 PTP gamma (PTP RG)
4 PTP beta (PTP B)
5

All lanes:

Western blot - Anti-PTPRK antibody (ab13225) at 1 µg/mL

Lane 1:

0.1µg GLEPP1 (PTPRO) protein

Lane 2:

0.1µg SAP 1 (PTPRH) protein

Lane 3:

0.1µg PTP gamma (PTPRG) protein

Lane 4:

0.1µg PTP beta (PTPRB) protein

Lane 5:

0.1µg DEP1 (PTPRJ) protein

Lane 6:

0.1µg PTP kappa (PTPRK) protein

Lane 7:

0.1µg PCPTP1 (PTPRR) protein

Lane 8:

0.1µg LAR (PTPRF) protein

Predicted band size: 162 kDa

Observed band size: 65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTPRK also known as receptor-type tyrosine-protein phosphatase kappa is a protein involved in dephosphorylation processes. It has a molecular weight of approximately 145 kDa. The protein functions as a dimer and is encoded by the PTPRK gene. It is widely expressed in various tissues like the skin testes and colon. PTPRK interacts with other proteins at its extracellular region influencing cell adhesion and growth.

Biological function summary

PTPRK plays a role in regulating cell signaling by removing phosphate groups from phosphotyrosine residues on proteins. This activity influences cell growth differentiation and cellular communication. As a receptor-type protein tyrosine phosphatase it acts as a mediator in cell-cell adhesion and contact inhibition of cell growth. The protein is involved in the regulation of E-cadherin-mediated adhesion suggesting its importance in cell junctions.

Pathways

PTPRK is an important component in the Hedgehog and Wnt signaling pathways. These pathways are essential for embryonic development and maintenance of adult tissue homeostasis. PTPRK interacts with the calcium-dependent cell adhesion protein E-cadherin to mediate signal transduction within these pathways. The interplay with these pathways suggests its involvement in maintaining cellular architecture and function.

PTPRK has been associated with cancer and autoimmune diseases. Dysregulation of PTPRK expression or function can contribute to the development of colorectal cancer. Moreover altered PTPRK activity is implicated in psoriasis an autoimmune skin disorder where it is linked through an association with altered E-cadherin expression. These connections highlight the role of PTPRK in disease pathology and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Regulation of processes involving cell contact and adhesion such as growth control, tumor invasion, and metastasis. Negative regulator of EGFR signaling pathway. Forms complexes with beta-catenin and gamma-catenin/plakoglobin. Beta-catenin may be a substrate for the catalytic activity of PTPRK/PTP-kappa.

See full target information PTPRK

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 8:e62852 PubMed23696788

2013

Tumor derived mutations of protein tyrosine phosphatase receptor type k affect its function and alter sensitivity to chemotherapeutics in glioma.

Applications

Species

Human

Supreet Agarwal,Maha S Al-Keilani,Mohammad A Y Alqudah,Zita A Sibenaller,Timothy C Ryken,Mahfoud Assem

Blood 111:292-301 PubMed17720884

2007

Down-regulation of the TGF-beta target gene, PTPRK, by the Epstein-Barr virus encoded EBNA1 contributes to the growth and survival of Hodgkin lymphoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne R Flavell,Karl R N Baumforth,Victoria H J Wood,Gillian L Davies,Wenbin Wei,Gary M Reynolds,Susan Morgan,Andrew Boyce,Gemma L Kelly,Lawrence S Young,Paul G Murray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PTPRK antibody

Western blot - Anti-PTPRK antibody (AB13225)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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