Rabbit Polyclonal PTPRN2 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PTPRN2.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PTPRN2. The exact immunogen used to generate this antibody is proprietary information. Database link Q92932
Reactivity data
Select an application| WB | |
|---|---|
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
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Target data
Function
Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) (By similarity). Required to maintain normal levels of renin expression and renin release (By similarity). May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization (By similarity). Has phosphatidylinositol phosphatase activity; the PIPase activity is involved in its ability to regulate insulin secretion. Can dephosphorylate phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), phosphatidylinositol 5-phosphate and phosphatidylinositol 3-phosphate (By similarity). Regulates PI(4,5)P2 level in the plasma membrane and localization of cofilin at the plasma membrane and thus is indirectly involved in regulation of actin dynamics related to cell migration and metastasis; upon hydrolyzation of PI(4,5)P2 cofilin is released from the plasma membrane and acts in the cytoplasm in severing F-actin filaments (PubMed:26620550).
Alternative names
KIAA0387, PTPRN2, Receptor-type tyrosine-protein phosphatase N2, R-PTP-N2, Islet cell autoantigen-related protein, Phogrin, IAR, ICAAR
Recommended products
Rabbit Polyclonal PTPRN2 antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human PTPRN2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PTPRN2. The exact immunogen used to generate this antibody is proprietary information. Database link Q92932
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using an epitope specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PTPRN2 also known as IA-2β or Islet Antigen 2 β is a protein tyrosine phosphatase receptor. It has a mass of approximately 109 kDa. PTPRN2 expresses mainly in neuroendocrine tissues including the pancreas and brain. This protein is involved in vesicular transport and is thought to play a role in the maintenance and function of dense core vesicles. It has a single transmembrane domain and is closely related to other members of the protein tyrosine phosphatase family.
- Biological function summary
PTPRN2 participates in the regulation and secretion of hormones and neurotransmitters. It is an integral part of the vesicle trafficking complex impacting the exocytosis process. Studies show that PTPRN2 influences the storage and release of insulin and other neurotransmitters by stabilizing secretory granules. This protein’s expression pattern suggests it may also have roles in neuronal communication and hormone modulation.
- Pathways
Research shows PTPRN2 influences the insulin secretory pathway and neuropeptide signaling. It interacts with other proteins like SNAP25 and Synaptotagmin which are involved in synaptic vesicle fusion and neurotransmitter release. PTPRN2’s role in these pathways indicates its importance in communication in neuronal and endocrine systems linking it to broader signaling networks.
- Associated diseases and disorders
PTPRN2 has connections to type 1 diabetes and neurodegenerative diseases. Autoantibodies against PTPRN2 are often detected in patients with type 1 diabetes suggesting its involvement in autoimmune reactions. Additionally abnormal expression or function of PTPRN2 is observed in neurodegenerative conditions like Alzheimer's disease. This highlights its potential role in the progression of these diseases possibly through interaction with neuroprotective proteins or pathways that involve stress responses in neuroendocrine cells.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PTPRN2 antibody (ab92652)
All lanes: Western blot - Anti-PTPRN2 antibody (ab92652) at 1/500 dilution
Lane 1: Extract from NIH/3T3 cells (without competing peptide) at 5 µg
Lane 2: Extract from NIH/3T3 cells (with competing peptide) at 5 µg
Predicted band size: 111 kDa
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