AB76919

Anti-PTRF antibody

Name: Anti-PTRF antibody (ab76919) | Abcam
Brand: Abcam Limited
SKU: AB76919
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications )

Rabbit Polyclonal PTRF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CAVIN1.

2 Images

Unknown

Immunoprecipitation - Anti-PTRF antibody (AB76919)

Western Blot of Immunprecipitate.
ab76919 at 1µg/ml (for WB) staining PTRF in HeLa whole cell lysate,
immunoprecipitated using ab76919 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-PTRF antibody (ab76919)

Predicted band size: 43 kDa

false

Unknown

Western blot - Anti-PTRF antibody (AB76919)

All lanes:

Western blot - Anti-PTRF antibody (ab76919) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 43 kDa

Observed band size: 51 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CAVIN1. The exact immunogen used to generate this antibody is proprietary information.

Q6NZI2

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTRF also known as Cavin-1 is a protein with a mass of approximately 43 kDa. It functions primarily as a regulator of caveolae formation and stability. Caveolae are small flask-shaped invaginations in the plasma membrane. PTRF interacts directly with caveolin proteins to facilitate caveolae assembly. It is largely expressed in adipose tissue muscle tissue and endothelial cells indicating a significant role in cellular processes in these cells.

Biological function summary

PTRF facilitates various processes like signal transduction and lipid metabolism due to its role in caveolae function. As part of the caveolae complex it is essential for maintaining structural integrity and organization. PTRF interacts with other proteins such as cavin family members and caveolins aiding in cellular response to mechanochemical stimuli. By regulating the caveolae PTRF influences activities including endocytosis and potocytosis.

Pathways

PTRF is critical in cholesterol homeostasis and endothelial nitric oxide synthase (eNOS) signaling pathways. It associates with caveolin-1 modulating these pathways to balance lipid composition and promote endothelial function. The interaction between PTRF and caveolin-1 enhances nitric oxide production which is important for vascular tone regulation and cellular stress response. This positions PTRF as a regulator in cardiovascular and metabolic pathways.

Defects or deficiencies in PTRF can lead to disorders such as lipodystrophy and muscular dystrophy. The absence or malfunction of PTRF disrupts normal caveolae structure leading to impaired lipid storage and muscle function. PTRF's interaction with caveolin-1 connects it to cardiovascular diseases as caveolin-1 mutations contribute to the pathogenesis of cardiac dysfunction. Understanding PTRF's role offers insights into therapeutic strategies for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed : 18056712, PubMed : 18191225, PubMed : 19726876). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity) (PubMed : 18056712, PubMed : 18191225, PubMed : 19726876). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).

See full target information CAVIN1

Alternative Names

PTRF, FKSG13, CAVIN1, Caveolae-associated protein 1, Cavin-1, Polymerase I and transcript release factor

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:104574 PubMed36870682

2023

Structural analysis of the P132L disease mutation in caveolin-1 reveals its role in the assembly of oligomeric complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Han,Alican Gulsevin,Sarah Connolly,Ting Wang,Brigitte Meyer,Jason Porta,Ajit Tiwari,Angie Deng,Louise Chang,Yelena Peskova,Hassane S Mchaourab,Erkan Karakas,Melanie D Ohi,Jens Meiler,Anne K Kenworthy

Frontiers in molecular biosciences 8:705148 PubMed35071320

2022

Development and Verification of a Hypoxic Gene Signature for Predicting Prognosis, Immune Microenvironment, and Chemosensitivity for Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fengfeng Wu,Juntao Xu,Mingchao Jin,Xuesheng Jiang,Jianyou Li,Xiongfeng Li,Zhuo Chen,Jiangbo Nie,Zhipeng Meng,Guorong Wang

Nature communications 7:13267 PubMed27827363

2016

Control of diabetic hyperglycaemia and insulin resistance through TSC22D4.

Applications

Unspecified application

Species

Unspecified reactive species

Bilgen Ekim Üstünel,Kilian Friedrich,Adriano Maida,Xiaoyue Wang,Anja Krones-Herzig,Oksana Seibert,Anke Sommerfeld,Allan Jones,Tjeerd P Sijmonsma,Carsten Sticht,Norbert Gretz,Thomas Fleming,Peter P Nawroth,Wolfgang Stremmel,Adam J Rose,Mauricio Berriel-Diaz,Matthias Blüher,Stephan Herzig

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTRF antibody

Immunoprecipitation - Anti-PTRF antibody (AB76919)

Western blot - Anti-PTRF antibody (AB76919)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (3)

Product promise