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AB312883

Anti-PTRF antibody [EPR27006-32] - BSA and Azide free (Detector)

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Knockout Tested Rabbit Recombinant Monoclonal PTRF antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

PTRF, FKSG13, CAVIN1, Caveolae-associated protein 1, Cavin-1, Polymerase I and transcript release factor

1 Images
Sandwich ELISA - Anti-PTRF antibody [EPR27006-32] - BSA and Azide free (Detector) (AB312883)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-PTRF antibody [EPR27006-32] - BSA and Azide free (Detector) (AB312883)

Sandwich ELISA of ab312881 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27006-32

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTRF also known as Cavin-1 is a protein with a mass of approximately 43 kDa. It functions primarily as a regulator of caveolae formation and stability. Caveolae are small flask-shaped invaginations in the plasma membrane. PTRF interacts directly with caveolin proteins to facilitate caveolae assembly. It is largely expressed in adipose tissue muscle tissue and endothelial cells indicating a significant role in cellular processes in these cells.
Biological function summary

PTRF facilitates various processes like signal transduction and lipid metabolism due to its role in caveolae function. As part of the caveolae complex it is essential for maintaining structural integrity and organization. PTRF interacts with other proteins such as cavin family members and caveolins aiding in cellular response to mechanochemical stimuli. By regulating the caveolae PTRF influences activities including endocytosis and potocytosis.

Pathways

PTRF is critical in cholesterol homeostasis and endothelial nitric oxide synthase (eNOS) signaling pathways. It associates with caveolin-1 modulating these pathways to balance lipid composition and promote endothelial function. The interaction between PTRF and caveolin-1 enhances nitric oxide production which is important for vascular tone regulation and cellular stress response. This positions PTRF as a regulator in cardiovascular and metabolic pathways.

Defects or deficiencies in PTRF can lead to disorders such as lipodystrophy and muscular dystrophy. The absence or malfunction of PTRF disrupts normal caveolae structure leading to impaired lipid storage and muscle function. PTRF's interaction with caveolin-1 connects it to cardiovascular diseases as caveolin-1 mutations contribute to the pathogenesis of cardiac dysfunction. Understanding PTRF's role offers insights into therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in caveolae formation and organization. Essential for the formation of caveolae in all tissues (PubMed : 18056712, PubMed : 18191225, PubMed : 19726876). Core component of the CAVIN complex which is essential for recruitment of the complex to the caveolae in presence of calveolin-1 (CAV1). Essential for normal oligomerization of CAV1. Promotes ribosomal transcriptional activity in response to metabolic challenges in the adipocytes and plays an important role in the formation of the ribosomal transcriptional loop. Dissociates transcription complexes paused by DNA-bound TTF1, thereby releasing both RNA polymerase I and pre-RNA from the template (By similarity) (PubMed : 18056712, PubMed : 18191225, PubMed : 19726876). The caveolae biogenesis pathway is required for the secretion of proteins such as GASK1A (By similarity).
See full target information CAVIN1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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