AB96104

Anti-PTS/PTPS antibody

Name: Anti-PTS/PTPS antibody (ab96104) | Abcam
Brand: Abcam Limited
SKU: AB96104
Rating: 3 (1 reviews)

(1 Review)

(1 Publication)

Rabbit Polyclonal PTS/PTPS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human 6-pyruvoyl tetrahydrobiopterin synthase aa 1 to C-terminus.

View Alternative Names

6-pyruvoyl tetrahydrobiopterin synthase, PTP synthase, PTPS, PTS

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS antibody (AB96104)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS antibody (AB96104)

Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling PTS (green) with ab96104 at 1/100 dilution. Costained with Hoechst 33342 (Bottom Panel).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS antibody (AB96104)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS antibody (AB96104)

Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining PTS with ab96104 at 1/500 dilution.
Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Unknown

Western blot - Anti-PTS/PTPS antibody (AB96104)

15% SDS PAGE

All lanes:

Western blot - Anti-PTS/PTPS antibody (ab96104) at 1/1000 dilution

All lanes:

H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 16 kDa

false

Lab

Western blot - Anti-PTS/PTPS antibody (AB96104)

15% SDS-PAGE

All lanes:

Western blot - Anti-PTS/PTPS antibody (ab96104) at 1/500 dilution

Lane 1:

WT HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Lane 2:

PTS/PTPS Knockout HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-PTS/PTPS antibody (AB96104)

15% SDS PAGE

All lanes:

Western blot - Anti-PTS/PTPS antibody (ab96104) at 1/1000 dilution

All lanes:

Mouse stomach tissue lysate at 50 µg

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-PTS/PTPS antibody (AB96104)

15% SDS-PAGE

All lanes:

Western blot - Anti-PTS/PTPS antibody (ab96104) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Transfected HEK-293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human 6-pyruvoyl tetrahydrobiopterin synthase aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q03393

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTS/PTPS also known as 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase is an enzyme involved in the biosynthesis of tetrahydrobiopterin (BH4). Its molecular weight is approximately 23 kDa. The enzyme is expressed in various tissues including the liver kidney and brain. The enzyme catalyzes a step in the conversion of 78-dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin which is an important step in the BH4 production pathway.

Biological function summary

PTS/PTPS plays a significant role in neurotransmitter synthesis and regulation. BH4 acts as a cofactor for several hydroxylase enzymes these enzymes include phenylalanine hydroxylase tyrosine hydroxylase and tryptophan hydroxylase. PTS/PTPS contributes to the production of neurotransmitters like dopamine serotonin and nitric oxide through its role in the biosynthesis of BH4. The protein functions as a part of a complex that includes other biosynthetic enzymes involved in the same pathway.

Pathways

PTS/PTPS is essential in the pathways involved in neurotransmitter metabolism and amino acid hydroxylation. The enzyme is intimately associated with the phenylalanine tyrosine and tryptophan metabolic pathways. In these pathways it interacts with proteins such as phenylalanine hydroxylase and nitric oxide synthase supporting the conversion of amino acids into critical neurotransmitters and molecules for physiological processes.

PTS/PTPS dysfunction links to neurological conditions and metabolic disorders. Mutations or deficiencies in the enzyme are associated with hyperphenylalaninemia due to BH4 deficiency impacting phenylalanine metabolism and leading to severe neurological symptoms. Another related disorder is dystonia a movement disorder where altered dopamine biosynthesis occurs. The interactions with enzymes like phenylalanine hydroxylase become disrupted further influencing the disease mechanisms and contributing to symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

See full target information 6-pyruvoyl tetrahydrobiopterin synthase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 11:1912-1925 PubMed36248333

2022

is activated by and promotes lung adenocarcinoma development via the Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ma,Chao Wang,Ruzhen Li,Zhaohui Han,Yuanzhu Jiang,Xiangwei Zhang,Duilio Divisi,Enrico Capobianco,Lin Zhang,Wei Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTS/PTPS antibody

Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS antibody (AB96104)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS antibody (AB96104)

Western blot - Anti-PTS/PTPS antibody (AB96104)

Western blot - Anti-PTS/PTPS antibody (AB96104)

Western blot - Anti-PTS/PTPS antibody (AB96104)

Western blot - Anti-PTS/PTPS antibody (AB96104)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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