AB109462

Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal PTS/PTPS phospho S19 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

ab109462 at a 1/250 dilution staining PTS/PTPS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

ab109462 at a 1/250 dilution staining PTS/PTPS (phospho S19) in HeLa cells by Immunofluorescence.

Unknown

Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

All lanes:

Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution

Lane 1:

HUVEC cell lysate at 10 µg

Lane 2:

HUVEC cell lysate treated with Alkaline Phosphatase at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 16 kDa

Observed band size: 17 kDa

false

Carrier free

Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1517(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTS/PTPS also known as 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase is an enzyme involved in the biosynthesis of tetrahydrobiopterin (BH4). Its molecular weight is approximately 23 kDa. The enzyme is expressed in various tissues including the liver kidney and brain. The enzyme catalyzes a step in the conversion of 78-dihydroneopterin triphosphate to 6-pyruvoyl tetrahydropterin which is an important step in the BH4 production pathway.

Biological function summary

PTS/PTPS plays a significant role in neurotransmitter synthesis and regulation. BH4 acts as a cofactor for several hydroxylase enzymes these enzymes include phenylalanine hydroxylase tyrosine hydroxylase and tryptophan hydroxylase. PTS/PTPS contributes to the production of neurotransmitters like dopamine serotonin and nitric oxide through its role in the biosynthesis of BH4. The protein functions as a part of a complex that includes other biosynthetic enzymes involved in the same pathway.

Pathways

PTS/PTPS is essential in the pathways involved in neurotransmitter metabolism and amino acid hydroxylation. The enzyme is intimately associated with the phenylalanine tyrosine and tryptophan metabolic pathways. In these pathways it interacts with proteins such as phenylalanine hydroxylase and nitric oxide synthase supporting the conversion of amino acids into critical neurotransmitters and molecules for physiological processes.

PTS/PTPS dysfunction links to neurological conditions and metabolic disorders. Mutations or deficiencies in the enzyme are associated with hyperphenylalaninemia due to BH4 deficiency impacting phenylalanine metabolism and leading to severe neurological symptoms. Another related disorder is dystonia a movement disorder where altered dopamine biosynthesis occurs. The interactions with enzymes like phenylalanine hydroxylase become disrupted further influencing the disease mechanisms and contributing to symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl tetrahydropterin.

See full target information PTS pS19

Alternative Names

6-pyruvoyl tetrahydrobiopterin synthase, PTP synthase, PTPS, PTS

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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Pharmaceuticals (Basel, Switzerland) 18: PubMed41011190

2025

Nagilactone C from the Seeds of May Protect Against LPS-Induced Acute Lung Injury via STAT Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Chen,Jing Tang,Shijie Zhan,Yixian Qiu,Jing Li,Weiguang Shan,Youmin Ying

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (AB109462)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise