AB88082

Anti-PU.1/Spi1 antibody [2G1]

Name: Anti-PU.1/Spi1 antibody [2G1] Mouse monoclonal (ab88082) | Abcam
Brand: Abcam Limited
SKU: AB88082
Price: 650 USD
Availability: InStock

Be the first to review this product! Submit a review

(13 Publications)

Mouse Monoclonal PU.1/Spi1 antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 13 publications.

View Alternative Names

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

ab88082, at 10 μg/ml, staining PU.1/Spi1 in HeLa cells by Immunofluorescence.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Flow Cyt

Unknown

Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Overlay histogram showing K562 cells stained with ab88082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab88082, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

ab88082 staining PU.1/Spi1 in human induced microglia by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde for 15 minutes. Samples were incubated with primary antibody (1 : 200) for 2 hours at room temperature. Hoechst nuclear stain (blue) was used at 1 : 5000 dilution.

This image was generated using the ascites version of the product.

Image courtesy of Sellgren et al. Mol Psychiatry. 2017 Feb; 22(2): 170–177. Fig S3: Doi: 10.1038/mp.2016.220

ELISA - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

ELISA

Unknown

ELISA - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Detection limit for recombinant (human) tagged ab88082 is approximately 0.1ng/ml as a capture antibody.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082) at 5 µg/mL

All lanes:

Raw 264.7 cell lysate (Mouse) at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 31 kDa

Observed band size: 31 kDa

false

Unknown

Western blot - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Note : Molecular weight of the proprietary tag alone is 26 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082) at 5 µg/mL

All lanes:

tagged human recombinant protein fragment (the immunogen) at 0.2 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size: 31 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G1

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt, ELISA, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.

Biological function summary

PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.

Pathways

Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.

PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed : 23658224, PubMed : 33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed : 33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed : 33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

See full target information SPI1

style

left-column

Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Annals of the rheumatic diseases 83:1707-1721 PubMed39164066

2024

Positive feedback loop PU.1-IL9 in Th9 promotes rheumatoid arthritis development.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajie Tu,Weile Chen,Wei Huang,Xinming Wang,Yilong Fang,Xuming Wu,Huiru Zhang,Chong Liu,Xuewen Tan,Xiangling Zhu,Huihui Wang,Dafei Han,Yizhao Chen,Anqi Wang,Yuanyuan Zhou,Zimeng Xue,Hui Xue,Shangxue Yan,Lingling Zhang,Zhenbao Li,Chunlan Yang,Yujie Deng,Shihao Zhang,Chen Zhu,Wei Wei

Science advances 10:eadn8963 PubMed38838144

2024

Nucleoporin Nup358 drives the differentiation of myeloid-biased multipotent progenitors by modulating HDAC3 nuclear translocation.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Guglielmi,Davina Lam,Maximiliano A D'Angelo

iScience 26:107530 PubMed37664628

2023

R235C point mutation confers hypersensitivity to radiation-induced acute myeloid leukemia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Brown,Rosemary Finnon,Paul Finnon,Roisin McCarron,Lourdes Cruz-Garcia,Grainne O'Brien,Eleanor Herbert,Cheryl L Scudamore,Edouard Morel,Christophe Badie

International journal of molecular sciences 24: PubMed36768614

2023

miRNA-124 Prevents Rat Diabetic Retinopathy by Inhibiting the Microglial Inflammatory Response.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Andrea Schlotterer,Luke Kurowski,Lin Li,Marcus Dannehl,Hans-Peter Hammes,Jihong Lin

BMC immunology 24:4 PubMed36650424

2023

PU.1-CD23 signaling mediates pulmonary innate immunity against Aspergillus fumigatus infection by driving inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Ming Zhang,Jiayong Qiu,Chenyang Liu,Yao Lou,Tongsheng Wang,Yingmin Zhang,Yimin Mao

Nature communications 13:7677 PubMed36509766

2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Li,Liren Zhang,Wenhua You,Jiali Xu,Jingjing Dai,Dongxu Hua,Ruizhi Zhang,Feifan Yao,Suiqing Zhou,Wei Huang,Yongjiu Dai,Yu Zhang,Tasiken Baheti,Xiaofeng Qian,Liyong Pu,Jing Xu,Yongxiang Xia,Chuanyong Zhang,Jinhai Tang,Xuehao Wang

International journal of molecular sciences 23: PubMed35743167

2022

Combined Approach to Leukemic Differentiation Using Transcription Factor PU.1-Enhancing Agents.

Applications

Unspecified application

Species

Unspecified reactive species

Petra Bašová,Helena Paszeková,Lubomír Minařík,Martina Dluhošová,Pavel Burda,Tomáš Stopka

Journal of neuroinflammation 18:294 PubMed34920745

2021

A new method for obtaining bankable and expandable adult-like microglia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Jung You,Chan Rim,Youn-Jung Kang,Min-Soo Kwon

International journal of molecular sciences 22: PubMed34681723

2021

MicroRNA-124 Alleviates Retinal Vasoregression via Regulating Microglial Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Chen,Jihong Lin,Andrea Schlotterer,Luke Kurowski,Sigrid Hoffmann,Seddik Hammad,Steven Dooley,Malte Buchholz,Jiong Hu,Ingrid Fleming,Hans-Peter Hammes

Frontiers in cellular neuroscience 15:629279 PubMed33897370

2021

Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Atoshi Banerjee,Yimei Lu,Kenny Do,Travis Mize,Xiaogang Wu,Xiangning Chen,Jingchun Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PU.1/Spi1 antibody [2G1]

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

ELISA - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Western blot - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Western blot - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise