Mouse Monoclonal PU.1/Spi1 antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 9 publications.
Images
![Western blot - Anti-PU.1/Spi1 antibody [2G1] (AB88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--western-blot-img36961.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PU.1/Spi1 antibody [2G1] (AB88082)")
![Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--immunocytochemistry-immunofluorescence-img56933.png/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)")
![Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--immunocytochemistry-immunofluorescence-img97868.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)")
![ELISA - Anti-PU.1/Spi1 antibody [2G1] (AB88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--elisa-img61177.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-PU.1/Spi1 antibody [2G1] (AB88082)")
![Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (AB88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--flow-cytometry-img80344.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (AB88082)")
Publications
Filter by
- International journal of molecular sciences 24:2023miRNA-124 Prevents Rat Diabetic Retinopathy by Inhibiting the Microglial Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Andrea Schlotterer,Luke Kurowski,Lin Li,Marcus Dannehl,Hans-Peter Hammes,Jihong LinPubMed 36768614
- Nature communications 13:76772022PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Li,Liren Zhang,Wenhua You,Jiali Xu,Jingjing Dai,Dongxu Hua,Ruizhi Zhang,Feifan Yao,Suiqing Zhou,Wei Huang,Yongjiu Dai,Yu Zhang,Tasiken Baheti,Xiaofeng Qian,Liyong Pu,Jing Xu,Yongxiang Xia,Chuanyong Zhang,Jinhai Tang,Xuehao WangPubMed 36509766
- International journal of molecular sciences 23:2022Combined Approach to Leukemic Differentiation Using Transcription Factor PU.1-Enhancing Agents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetra Bašová,Helena Paszeková,Lubomír Minařík,Martina Dluhošová,Pavel Burda,Tomáš StopkaPubMed 35743167
- Journal of neuroinflammation 18:2942021A new method for obtaining bankable and expandable adult-like microglia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Jung You,Chan Rim,Youn-Jung Kang,Min-Soo KwonPubMed 34920745
- International journal of molecular sciences 22:2021MicroRNA-124 Alleviates Retinal Vasoregression via Regulating Microglial Polarization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Chen,Jihong Lin,Andrea Schlotterer,Luke Kurowski,Sigrid Hoffmann,Seddik Hammad,Steven Dooley,Malte Buchholz,Jiong Hu,Ingrid Fleming,Hans-Peter HammesPubMed 34681723
- Frontiers in cellular neuroscience 15:6292792021Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAtoshi Banerjee,Yimei Lu,Kenny Do,Travis Mize,Xiaogang Wu,Xiangning Chen,Jingchun ChenPubMed 33897370
- Oxidative medicine and cellular longevity 2021:55215032021Bu Shen Yi Sui Capsule Alleviates Neuroinflammation and Demyelination by Promoting Microglia toward M2 Polarization, Which Correlates with Changes in miR-124 and miR-155 in Experimental Autoimmune Encephalomyelitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Zha,Yan-Fang Gao,Jing Ji,Ya-Qin Sun,Jun-Ling Li,Fang Qi,Nan Zhang,Liang-Yun Jin,Bing Xue,Tao Yang,Yong-Ping Fan,Hui Zhao,Lei WangPubMed 33815654
- Molecular psychiatry 22:170-1772016Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors.Applications:ICC/IFReactive species:HumanC M Sellgren,S D Sheridan,J Gracias,D Xuan,T Fu,R H PerlisPubMed 27956744
- Leukemia 28:1436-482014PU.1 is essential for MLL leukemia partially via crosstalk with the MEIS/HOX pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Zhou,J Wu,B Li,D Liu,J Yu,X Yan,S Zheng,J Wang,L Zhang,L Zhang,F He,Q Li,A Chen,Y Zhang,X Zhao,Y Guan,X Zhao,J Yan,J Ni,M A Nobrega,B Löwenberg,R Delwel,P J M Valk,A Kumar,L Xie,D G Tenen,G Huang,Q-F WangPubMed 24445817
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | ELISA | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
Target data
Function
Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
Alternative names
Transcription factor PU.1, 31 kDa-transforming protein, SPI1
Recommended products
Mouse Monoclonal PU.1/Spi1 antibody. Carrier free. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse, Recombinant full length protein - Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 2G1
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.
- Biological function summary
PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.
- Pathways
Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.
- Associated diseases and disorders
PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--western-blot-img36961.jpg "Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
Note: Molecular weight of the proprietary tag alone is 26 kDa.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082) at 5 µg/mL
All lanes: tagged human recombinant protein fragment (the immunogen) at 0.2 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 31 kDa
Observed band size: 39 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--immunocytochemistry-immunofluorescence-img56933.png "Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
Image courtesy of Sellgren et al. Mol Psychiatry. 2017 Feb; 22(2): 170–177. Fig S3: Doi: 10.1038/mp.2016.220Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
ab88082 staining PU.1/Spi1 in human induced microglia by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde for 15 minutes. Samples were incubated with primary antibody (1:200) for 2 hours at room temperature. Hoechst nuclear stain (blue) was used at 1:5000 dilution.
This image was generated using the ascites version of the product.
![Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--immunocytochemistry-immunofluorescence-img97868.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
ab88082, at 10 μg/ml, staining PU.1/Spi1 in HeLa cells by Immunofluorescence.
This image was generated using the ascites version of the product.
![ELISA - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--elisa-img61177.jpg "ELISA - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
ELISA - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
Detection limit for recombinant (human) tagged ab88082 is approximately 0.1ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
![Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--flow-cytometry-img80344.jpg "Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
Flow Cytometry - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
Overlay histogram showing K562 cells stained with ab88082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab88082, 2μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
![Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--western-blot-img160927.jpg "Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082) at 5 µg/mL
All lanes: Raw 264.7 cell lysate (Mouse) at 25 µg
SecondaryAll lanes: Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
![Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082), expandable thumbnail](https://content.abcam.com/products/images/pu1-spi1-antibody-2g1-ab88082--western-blot-img160927.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PU.1/Spi1 antibody [2G1] (ab88082)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com