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AB230336

Anti-PU.1/Spi1 antibody

5

(1 Review)

|

(5 Publications)

Rabbit Polyclonal PU.1/Spi1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SPI1 aa 200-250.

View Alternative Names

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody (AB230336)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody (AB230336)

4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells stained for PU.1/Spi1 using ab230336 at 1/25 dilution in ICC/IF.

Western blot - Anti-PU.1/Spi1 antibody (AB230336)
  • WB

Supplier Data

Western blot - Anti-PU.1/Spi1 antibody (AB230336)

All lanes:

Western blot - Anti-PU.1/Spi1 antibody (ab230336) at 1/1000 dilution

All lanes:

WiDr cell lysate at 35 µg

Predicted band size: 31 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody (AB230336)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody (AB230336)

Paraffin-embedded, formalin fixed human spleen tissue stained for PU.1/Spi1 using ab230336 at 5μg/ml in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human SPI1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P17947

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/50", "ICCIF-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.
Biological function summary

PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.

Pathways

Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.

PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed : 23658224, PubMed : 33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed : 33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed : 33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
See full target information SPI1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Annals of the rheumatic diseases 83:1707-1721 PubMed39164066

2024

Positive feedback loop PU.1-IL9 in Th9 promotes rheumatoid arthritis development.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajie Tu,Weile Chen,Wei Huang,Xinming Wang,Yilong Fang,Xuming Wu,Huiru Zhang,Chong Liu,Xuewen Tan,Xiangling Zhu,Huihui Wang,Dafei Han,Yizhao Chen,Anqi Wang,Yuanyuan Zhou,Zimeng Xue,Hui Xue,Shangxue Yan,Lingling Zhang,Zhenbao Li,Chunlan Yang,Yujie Deng,Shihao Zhang,Chen Zhu,Wei Wei

Annals of the rheumatic diseases 82:198-211 PubMed36198439

2022

PU.1 promotes development of rheumatoid arthritis via repressing FLT3 in macrophages and fibroblast-like synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajie Tu,Weile Chen,Yilong Fang,Dafei Han,Yizhao Chen,Haifeng Jiang,Xuewen Tan,Zhen Xu,Xuming Wu,Huihui Wang,Xiangling Zhu,Wenming Hong,Zhenbao Li,Chen Zhu,Xinming Wang,Wei Wei

Biology 11: PubMed36101427

2022

Abnormal Gene Expression Regulation Mechanism of Myeloid Cell Nuclear Differentiation Antigen in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongxiang Tang,Lili Wang,Ousman Bajinka,Guojun Wu,Yurong Tan

Clinical and translational medicine 11:e588 PubMed34841706

2021

Therapeutic targeting of SPIB/SPI1-facilitated interplay of cancer cells and neutrophils inhibits aerobic glycolysis and cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqun Wang,Xiaojing Wang,Yanhua Guo,Lin Ye,Dan Li,Anpei Hu,Shuang Cai,Boling Yuan,Shikai Jin,Yi Zhou,Qilan Li,Liduan Zheng,Qiangsong Tong

Clinical and translational medicine : PubMed32628818

2020

Comprehensive analysis of 5-hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yanqi Dang,Dan Hu,Jingjuan Xu,Chunlin Li,Yingjue Tang,Zhenhua Yang,Yang Liu,Wenjun Zhou,Li Zhang,Hanchen Xu,Yangxian Xu,Guang Ji
View all publications
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Product promise

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