AB76543

Anti-PU.1/Spi1 antibody [EPR3158Y]

Name: Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) | Abcam
Brand: Abcam Limited
SKU: AB76543
Price: 365 USD
Availability: InStock
Rating: 5 (3 reviews)

BOND RX™ Validated
RabMAb
Recombinant
20ul selling size
What is this?

(3 Reviews)

(21 Publications)

Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) is a rabbit monoclonal antibody detecting PU.1/Spi1 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
- Trusted since 2009

View Alternative Names

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling PU.1/Spi1 with Purified ab76543 at 1 : 3000 dilution (0.035 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Unknown

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

PU.1 is a 37-42 kDa member of the ets family of transcription factors. The molecule weight observed is consistent with what has been described in the literature (PMID : 28362429, 31586032, 28586009).

All lanes:

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/1000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 2:

THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 37-42 kDa

false

Lab

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

Exposure time : Lane 1-2 : 180 seconds; Lane 3-4 : 20 seconds

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

ab76543 is more sensitive than ab302623 in WB testing.

Lanes 1 - 2:

Western blot - Anti-PU.1/Spi1 antibody [EPR25123-110] (<a href='/en-us/products/primary-antibodies/pu1-spi1-antibody-epr25123-110-ab302623'>ab302623</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot - Anti-PU.1/Spi1 antibody [EPR25123-110] (BSA and Azide free) (<a href='/en-us/products/primary-antibodies/pu1-spi1-antibody-epr25123-110-bsa-and-azide-free-ab302624'>ab302624</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/1000 dilution

Lanes 1 and 3:

Daudi whole cell lysate at 20 µg

Lanes 2 and 4:

Raji whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 37-42 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PU.1/Spi1 antibody [EPR3158Y]
Carrier free

Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3158Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/3000", "IHCP-species-notes": "For unpurified use: 1/100 - 1/250. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use: 1/5000 - 1/20000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

What is this antibody validated in?
Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of PU.1/Spi1?
Anti-PU.1/Spi1 [EPR3158Y] (ab76543) specifically detects a band for PU.1/Spi1 (UniProt: P17947) at a molecular weight of 31kDa.

Trusted by the scientific community
Anti-PU.1/Spi1 [EPR3158Y] (ab76543) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR3158Y] also available for your convenience: ab76543, Alexa Fluor® 647 - ab225040, Carrier free - ab232383

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.

Biological function summary

PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.

Pathways

Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.

PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed : 23658224, PubMed : 33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed : 33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed : 33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

See full target information SPI1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684718

2024

Monocyte/Macrophage-Specific Loss of ARNTL Suppresses Chronic Kidney Disease-Associated Cardiac Impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Yoshida,Naoki Nishikawa,Kohei Fukuoka,Akito Tsuruta,Kaita Otsuki,Taiki Fukuda,Yuma Terada,Tomohito Tanihara,Taisei Kumamoto,Ryotaro Tsukamoto,Takumi Nishi,Kosuke Oyama,Kengo Hamamura,Kouta Mayanagi,Satoru Koyanagi,Shigehiro Ohdo,Naoya Matsunaga

American journal of cancer research 14:1139-1156 PubMed38590399

2024

PU.1 induces tumor-associated macrophages promoting glioma progression through BTK-mediated Akt/mTOR pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Gu Song,Zeyu Zhang,Yan Chen,Weiliang Hou,Weiwei Zhong,Yuhang Zhou,Anke Zhang,Yuanzhi Xu

Journal of virology 98:e0170223 PubMed38078733

2023

Transcriptional regulation of the HIV-1 inhibitory factor human mannose receptor 1 by the myeloid-specific transcription factor PU.1.

Applications

Unspecified application

Species

Unspecified reactive species

Rosa Mallorson,Eri Miyagi,Sandra Kao,Sayaka Sukegawa,Hideki Saito,Helena Fabryova,Luciana Morellatto Ruggieri,Sonia Mediouni,Susana T Valente,Klaus Strebel

Methods in molecular biology (Clifton, N.J.) 2683:53-68 PubMed37300766

2023

Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Wei Chen,Yu-Hui Wong

JCI insight 7: PubMed35608913

2022

Isolevuglandins disrupt PU.1-mediated C1q expression and promote autoimmunity and hypertension in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

David M Patrick,Néstor de la Visitación,Jaya Krishnan,Wei Chen,Michelle J Ormseth,C Michael Stein,Sean S Davies,Venkataraman Amarnath,Leslie J Crofford,Jonathan M Williams,Shilin Zhao,Charles D Smart,Sergey Dikalov,Anna Dikalova,Liang Xiao,Justin P Van Beusecum,Mingfang Ao,Agnes B Fogo,Annet Kirabo,David G Harrison

Journal of virology 96:e0065222 PubMed35766490

2022

The Myeloid-Specific Transcription Factor PU.1 Upregulates Mannose Receptor Expression but Represses Basal Activity of the HIV-LTR Promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Kao,Eri Miyagi,Rosa Mallorson,Hideki Saito,Sayaka Sukegawa,Abhik Mukherji,Allyson Mateja,Damien Ferhadian,Helena Fabryova,Kathleen Clouse,Klaus Strebel

Aging 14:5390-5405 PubMed35771155

2022

MIR503HG impeded ovarian cancer progression by interacting with SPI1 and preventing TMEFF1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Tian,Lei Yang,Zhongtai Wang,Haiya Yan

Cells 11: PubMed35741044

2022

Challenges in Cell Fate Acquisition to Scid-Repopulating Activity from Hemogenic Endothelium of hiPSCs Derived from AML Patients Using Forced Transcription Factor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Deanna P Porras,Jennifer C Reid,Borko Tanasijevic,Diana Golubeva,Allison L Boyd,Mickie Bhatia

Frontiers in oncology 12:820568 PubMed35237521

2022

Comprehensive Analysis of Immune Implications and Prognostic Value of SPI1 in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfeng Huang,Wenzheng Chen,Zhigang Jie,Mengmeng Jiang

Cells 10: PubMed34831433

2021

Deleterious Role of Th9 Cells in Pulmonary Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Miao Deng,Xiang Sheng Yang,Qun Luo,Yi Xin She,Qing Yang Yu,Xiao Xiao Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PU.1/Spi1 antibody [EPR3158Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise