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Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) is a rabbit monoclonal antibody that is used to detect PU.1/Spi1 in Western Blot, IHC-P. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications

-Over 10 publications


Images

Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543), expandable thumbnail
  • Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/3000
Notes

For unpurified use: 1/100.00000 - 1/250.00000

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use: 1/5000.00000 - 1/20000.00000

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed:33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

Alternative names

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

Recommended products

Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) is a rabbit monoclonal antibody that is used to detect PU.1/Spi1 in Western Blot, IHC-P. Suitable for Human samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 10 publications

-Over 10 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3158Y
Purification technique
Affinity purification Protein A
Dissociation constant
4.4 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), in Human samples.

What is the molecular weight of PU.1/Spi1?


Anti-PU.1/Spi1 [EPR3158Y] (ab76543) specifically detects a band for PU.1/Spi1 (UniProt: P17947) at a molecular weight of 31kDa.

Recommended positive controls


WB: Daudi, THP-1 and Raji Whole cell lysateIHC: human tonsil tissue

Trusted by the scientific community


Anti-PU.1/Spi1 [EPR3158Y] (ab76543) was first used in a scientific publication in 2009 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR3158Y] also available for your convenience:
ab76543, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-PU.1/Spi1 antibody [EPR3158Y] ab225040, Carrier free - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free ab232383



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.

Biological function summary

PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.

Pathways

Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.

Associated diseases and disorders

PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543), expandable thumbnail

    Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

    PU.1 is a 37-42 kDa member of the ets family of transcription factors. The molecule weight observed is consistent with what has been described in the literature (PMID: 28362429, 31586032, 28586009).

    All lanes: Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/1000 dilution

    Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

    Lane 2: THP-1 (Human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 3: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa

    Observed band size: 37-42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling PU.1/Spi1 with Purified ab76543 at 1:3000 dilution (0.035 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543), expandable thumbnail

    Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543)

    PU.1/Spi1 Western blot staining using rabbit Anti-PU.1/Spi1 antibody

    Exposure time: Lane 1-2: 180 seconds; Lane 3-4: 20 seconds

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.

    ab76543 is more sensitive than Anti-PU.1/Spi1 antibody [EPR25123-110] ab302623 in WB testing.

    Lanes 1 - 2: Western blot - Anti-PU.1/Spi1 antibody [EPR25123-110] (Anti-PU.1/Spi1 antibody [EPR25123-110] ab302623) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-PU.1/Spi1 antibody [EPR25123-110] (BSA and Azide free) (Anti-PU.1/Spi1 antibody [EPR25123-110] (BSA and Azide free) ab302624) at 1/1000 dilution

    Lanes 3 - 4: Western blot - Anti-PU.1/Spi1 antibody [EPR3158Y] (ab76543) at 1/1000 dilution

    Lanes 1 and 3: Daudi whole cell lysate at 20 µg

    Lanes 2 and 4: Raji whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa

    Observed band size: 37-42 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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