JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183327

Anti-PU.1/Spi1 antibody [SP238]

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Recombinant Monoclonal PU.1/Spi1 antibody. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, ChIC/CUT&RUN-seq and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Transcription factor PU.1, 31 kDa-transforming protein, SPI1

6 Images
Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Flow Cytometry analysis of THP-1 (human monocytic leukemia monocyte) cells labeling PU.1/Spi1 with purified ab183327 at 1 : 200 dilution (0.715 μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PU.1/Spi1 with ab183327 at 1/100.

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)
  • ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [SP238] (AB183327)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [SP238] (AB183327)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488 ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Carrier free

    Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP238

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIC/CUT&RUN-seq, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PU.1 also known as Spi1 is a transcription factor that belongs to the ETS family of transcription factors. It plays a major role in gene regulation and is an important player in hematopoietic cell differentiation. PU.1 has a molecular mass of approximately 31 kDa and is predominantly expressed in hematopoietic cells such as myeloid and B-lymphoid cells. It actively interacts with specific DNA sequences to regulate the expression of target genes.
Biological function summary

PU.1 influences the development and function of various blood cells including macrophages neutrophils and B-cells. It is essential for the regulation of genes involved in immune responses cell proliferation and survival. PU.1 functions as part of a larger transcriptional regulatory complex and often partners with other transcription factors and coactivators to exert its effects. This cooperation allows precise gene expression control in specific cell lineages.

Pathways

Expression of PU.1 is critical in the hematopoietic development pathway and the immune response pathway. It closely interacts with other transcription factors like GATA-1 and C/EBPα forming a network that affects hematopoietic lineage commitment. Within these pathways PU.1 constantly coordinates signals that influence progenitor cell fate and differentiation ensuring a balanced proportion of cell types within the blood.

PU.1 has significant implications in leukemia and other hematological malignancies. Dysregulation or mutation of PU.1 can lead to improper hematopoietic cell development and contribute to acute myeloid leukemia (AML) and other blood disorders. Additionally its expression level is closely linked with immune system function associating PU.1 indirectly with autoimmune conditions. In these contexts the interaction with other proteins like AML1 and C/EBPγ can influence disease pathogenesis by modulating gene expression patterns critical for normal hematopoietic and immune function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed : 23658224, PubMed : 33951726). Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity). Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity). May be particularly important for the pro- to pre-B cell transition (PubMed : 33951726). Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed : 33951726). In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
See full target information SPI1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:163 PubMed38918792

2024

Maternal SARS-CoV-2 impacts fetal placental macrophage programs and placenta-derived microglial models of neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia L Shook,Rebecca E Batorsky,Rose M De Guzman,Liam T McCrea,Sara M Brigida,Joy E Horng,Steven D Sheridan,Olha Kholod,Aidan M Cook,Jonathan Z Li,Donna K Slonim,Brittany A Goods,Roy H Perlis,Andrea G Edlow

HemaSphere 8:e32 PubMed38434534

2024

CDK6 protein expression is associated with disease progression and treatment resistance in multiple myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Steinhart,Peter Möller,Miriam Kull,Jan Krönke,Thomas F E Barth

Biological psychiatry 95:676-686 PubMed37573007

2023

Loss of Function in the Neurodevelopmental Disease and Schizophrenia-Associated Gene CYFIP1 in Human Microglia-like Cells Supports a Functional Role in Synaptic Engulfment.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Joy E Horng,Hana Yeh,Liam McCrea,Jennifer Wang,Ting Fu,Roy H Perlis

Translational psychiatry 11:179 PubMed33741894

2021

Umbilical cord blood-derived microglia-like cells to model COVID-19 exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Jessica M Thanos,Rose M De Guzman,Liam T McCrea,Joy E Horng,Ting Fu,Carl M Sellgren,Roy H Perlis,Andrea G Edlow

bioRxiv : the preprint server for biology : PubMed33052344

2020

Umbilical cord blood derived microglia-like cells to model COVID-19 exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Steven D Sheridan,Jessica M Thanos,Rose M De Guzman,Liam T McCrea,Joy Horng,Ting Fu,Carl M Sellgren,Roy H Perlis,Andrea G Edlow
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB209983

Anti-PU.1/Spi1 antibody

IHC - Wholemount - Anti-PU.1/Spi1 antibody (AB209983)

  • 0
  • 0
Primary Antibodies

AB238054

Anti-PU.1/Spi1 antibody [PU1/2146]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] (AB238054)

  • 0
  • 0
Primary Antibodies

AB237905

Anti-PU.1/Spi1 antibody [PU1/2146] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [PU1/2146] - BSA and Azide free (AB237905)

  • 0
  • 0
Primary Antibodies

AB88082

Anti-PU.1/Spi1 antibody [2G1]

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [2G1] (AB88082)

  • 0
  • 0
Primary Antibodies

AB225040

Alexa Fluor® 647 Anti-PU.1/Spi1 antibody [EPR3158Y]

RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Alexa Fluor® 647 Anti-PU.1/Spi1 antibody [EPR3158Y] (AB225040)

  • 0
  • 0
Primary Antibodies

AB76543

Anti-PU.1/Spi1 antibody [EPR3158Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] (AB76543)

  • 5
  • 3
Primary Antibodies

AB264473

Anti-PU.1/Spi1 antibody [EPR22624-20] - ChIP Grade - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [EPR22624-20] - ChIP Grade - BSA and Azide free (AB264473)

  • 0
  • 0
Primary Antibodies

AB227835

Anti-PU.1/Spi1 antibody [EPR22624-20] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [EPR22624-20] - ChIP Grade (AB227835)

  • 0
  • 0
Primary Antibodies

AB232383

Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [EPR3158Y] - BSA and Azide free (AB232383)

  • 0
  • 0
Primary Antibodies

AB230336

Anti-PU.1/Spi1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody (AB230336)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 49
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com