Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free (AB271940)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183327).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free (AB271940)

Intracellular Flow Cytometry analysis of THP-1 (human monocytic leukemia monocyte) cells labeling PU.1/Spi1 with purified ab183327 at 1/200 dilution (0.715μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^© 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183327).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free (AB271940)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling PU.1/Spi1 with ab183327 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183327).

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free (AB271940)

"This data was developed using the same antibody clone in a different buffer formulation (ab183327). ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free (AB271940)

"This data was developed using the same antibody clone in a different buffer formulation (ab183327). ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."

• ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-PU.1/Spi1 antibody [SP238] - BSA and Azide free (AB271940)

"This data was developed using the same antibody clone in a different buffer formulation (ab183327). ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 THP-1 (Human monocytic leukemia monocyte) cells and 5 µg of ab183327 [SP238]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods."