AB225705

Anti-PUF60/FIR antibody

Name: Anti-PUF60/FIR antibody (ab225705) | Abcam
Brand: Abcam Limited
SKU: AB225705

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal PUF60/FIR antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PUF60 aa 1-50.

View Alternative Names

FIR, ROBPI, SIAHBP1, PUF60, Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, Ro-binding protein 1, Siah-binding protein 1, FBP-interacting repressor, RoBP1, Siah-BP1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (AB225705)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (AB225705)

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PUF60/FIR using ab225705 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-PUF60/FIR antibody (AB225705)

PUF60/FIR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225705 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225705 at 0.4 μg/ml.

Lane 1 : ab225705 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PUF60/FIR antibody (ab225705)

Predicted band size: 60 kDa

false

Supplier Data

Western blot - Anti-PUF60/FIR antibody (AB225705)

All lanes:

Western blot - Anti-PUF60/FIR antibody (ab225705) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 60 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PUF60 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UHX1

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab225705 was affinity purified using an epitope specific to PUF60/FIR immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PUF60 also known as Far-Upstream Element-Binding Protein 1 (FUBP1) or FIR (FUSE-Binding Protein-Interacting Repressor) is a protein with a molecular mass of about 60 kDa. It expresses in various tissues with significant levels detectable in the nucleus where it functions. This protein plays a role in RNA processing and gene expression regulation by binding to nucleic acids.

Biological function summary

PUF60/FIR is essential for mRNA processing. It is part of the spliceosome complex where it interacts with other splicing factors to facilitate pre-mRNA splicing. PUF60/FIR regulates alternative splicing events which can influence gene expression profiles enabling response to different physiological conditions. It connects with the regulation of cell growth and differentiation through its splicing activities.

Pathways

PUF60/FIR participates in the regulation of transcription and mRNA processing pathways. These pathways include the transcription regulation of MYC a critical protein for cell proliferation and apoptosis. PUF60/FIR modulates the splicing and transcription of RNA polymerase II-dependent genes functioning in interaction with other proteins such as SF3B1 and hnRNP H. This interaction influences the transcriptional activity of gene networks involved in cell cycle progression and survival pathways.

PUF60/FIR has an association with cancer and developmental disorders. Altered expression or mutations in PUF60/FIR link to certain cancers like colorectal cancer due to dysregulation of splicing events. It is also implicated in Verheij syndrome a developmental disorder attributed to haploinsufficiency of PUF60/FIR. In these contexts it interacts with proteins involved in cell cycle regulation and apoptosis like p53 highlighting its broader role in maintaining proper cellular function and development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA.

See full target information PUF60

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular oncology (Dordrecht) 47:157-174 PubMed37632669

2023

Targeting PUF60 prevents tumor progression by retarding mRNA decay of oxidative phosphorylation in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cancan Zhang,Xiaoge Ni,Chunlin Tao,Ziyang Zhou,Fengmian Wang,Fei Gu,Xiaoxiao Cui,Shuheng Jiang,Qing Li,Huan Lu,Dongxue Li,Zhiyong Wu,Rong Zhang

Molecular medicine (Cambridge, Mass.) 28:134 PubMed36401176

2022

CircRNA AFF4 induced by KDM1A promotes osteogenic differentiation through FNDC5/Irisin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ansong Liu,Yong Chen,Da Zhong,Chenggong Wang,Mi Yu,Chao Liu,Zhijun Yang,Wenkang Chen,Ke Yin

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PUF60/FIR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (AB225705)

Immunoprecipitation - Anti-PUF60/FIR antibody (AB225705)

Western blot - Anti-PUF60/FIR antibody (AB225705)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise