Rabbit Polyclonal PUF60/FIR antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUF60 aa 1-50.
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Publications
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- Cellular oncology (Dordrecht) 47:157-1742023Targeting PUF60 prevents tumor progression by retarding mRNA decay of oxidative phosphorylation in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCancan Zhang,Xiaoge Ni,Chunlin Tao,Ziyang Zhou,Fengmian Wang,Fei Gu,Xiaoxiao Cui,Shuheng Jiang,Qing Li,Huan Lu,Dongxue Li,Zhiyong Wu,Rong ZhangPubMed 37632669
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human PUF60 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHX1
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
Alternative names
Poly(U)-binding-splicing factor PUF60, 60 kDa poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, Ro-binding protein 1, Siah-binding protein 1, FBP-interacting repressor, RoBP1, Siah-BP1, SIAHBP1, ROBPI, FIR, PUF60
Recommended products
Rabbit Polyclonal PUF60/FIR antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUF60 aa 1-50.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human PUF60 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHX1
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab225705 was affinity purified using an epitope specific to PUF60/FIR immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PUF60 also known as Far-Upstream Element-Binding Protein 1 (FUBP1) or FIR (FUSE-Binding Protein-Interacting Repressor) is a protein with a molecular mass of about 60 kDa. It expresses in various tissues with significant levels detectable in the nucleus where it functions. This protein plays a role in RNA processing and gene expression regulation by binding to nucleic acids.
- Biological function summary
PUF60/FIR is essential for mRNA processing. It is part of the spliceosome complex where it interacts with other splicing factors to facilitate pre-mRNA splicing. PUF60/FIR regulates alternative splicing events which can influence gene expression profiles enabling response to different physiological conditions. It connects with the regulation of cell growth and differentiation through its splicing activities.
- Pathways
PUF60/FIR participates in the regulation of transcription and mRNA processing pathways. These pathways include the transcription regulation of MYC a critical protein for cell proliferation and apoptosis. PUF60/FIR modulates the splicing and transcription of RNA polymerase II-dependent genes functioning in interaction with other proteins such as SF3B1 and hnRNP H. This interaction influences the transcriptional activity of gene networks involved in cell cycle progression and survival pathways.
- Associated diseases and disorders
PUF60/FIR has an association with cancer and developmental disorders. Altered expression or mutations in PUF60/FIR link to certain cancers like colorectal cancer due to dysregulation of splicing events. It is also implicated in Verheij syndrome a developmental disorder attributed to haploinsufficiency of PUF60/FIR. In these contexts it interacts with proteins involved in cell cycle regulation and apoptosis like p53 highlighting its broader role in maintaining proper cellular function and development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-PUF60/FIR antibody (ab225705)
All lanes: Western blot - Anti-PUF60/FIR antibody (ab225705) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUF60/FIR antibody (ab225705)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for PUF60/FIR using ab225705 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Immunoprecipitation - Anti-PUF60/FIR antibody (ab225705)
PUF60/FIR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225705 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225705 at 0.4 μg/ml.
Lane 1: ab225705 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-PUF60/FIR antibody (ab225705)
Predicted band size: 60 kDa
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