Rabbit Polyclonal PUMA antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 129 publications. Immunogen corresponding to Synthetic Peptide within Human BBC3 aa 150 to C-terminus.
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- Cellular & molecular biology letters 28:1042023Molecular mechanism and therapeutic potential of HDAC9 in intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Lei,Hui Lin,Deyao Shi,Pan Hong,Hui Song,Bomansaan Herman,Zhiwei Liao,Cao YangPubMed 38093179
- Cell death & disease 14:5432023Deletion of ARGLU1 causes global defects in alternative splicing in vivo and mouse cortical malformations primarily via apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFenyong Yao,Shisheng Huang,Jiahui Liu,Chunhua Tan,Mengqi Xu,Dengkui Wang,Maoqing Huang,Yiyao Zhu,Xingxu Huang,Shuijin HePubMed 37612280
- Cancer gene therapy 30:1414-142520233-Bromopyruvate overcomes cetuximab resistance in human colorectal cancer cells by inducing autophagy-dependent ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingchao Mu,Qin Zhang,Chenye Zhao,Xiaopeng Li,Zilu Chen,Xuejun Sun,Junhui YuPubMed 37558749
- Discover. Oncology 14:12023Inhibition of secretory leukocyte protease inhibitor (SLPI) promotes the PUMA-mediated apoptosis and chemosensitivity to cisplatin in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhijiang Wei,Guiying Liu,Rufu Jia,Wei Zhang,Li Li,Yuanyuan Zhang,Zhijing Wang,Xiyong BaiPubMed 36595102
- International journal of molecular sciences 23:2022Developing 3D Organoid Raft Cultures from Patient-Derived Xenografts as Rapid Models to Screen Efficacy of Experimental Therapeutics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrachi Bajpai,Nilam Sanjib Banerjee,Dianne W Moore,Hyung-Gyoon Kim,Farrukh Afaq,Carlo M Contreras,Martin J Heslin,Vishnu B Reddy,Shajan Peter,Sooryanarayana Varambally,Sameer Al Diffalha,Upender MannePubMed 36430867
- International journal of molecular sciences 23:2022Improving Reporter Gene Assay Methodology for Evaluating the Ability of Compounds to Restore P53 Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinle Han,Jun Du,Dandan Shi,Lingjie Li,Dandan Li,Kun Zhang,Suwen Lin,Jingzhong Zhu,Zoufang Huang,You Zhou,Zhengyu FangPubMed 36430341
- Experimental hematology & oncology 11:832022Thioredoxin-1 regulates self-renewal and differentiation of murine hematopoietic stem cells through p53 tumor suppressor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaima Jabbar,Parker Mathews,Xiaobei Wang,Pasupathi Sundaramoorthy,Emily Chu,Sadhna O Piryani,Shengli Ding,Xiling Shen,Phuong L Doan,Yubin KangPubMed 36316713
- Toxicological sciences : an official journal of the Society of Toxicology 190:23-402022Gestational Benzo[a]pyrene Exposure Destroys F1 Ovarian Germ Cells Through Mitochondrial Apoptosis Pathway and Diminishes Surviving Oocyte Quality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelli F Malott,Kathleen Leon Parada,Melody Lee,Edward Swanson,Ulrike LudererPubMed 35993611
- International journal of molecular sciences 23:2022Regulation and Role of Neuron-Derived Hemoglobin in the Mouse Hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujiao Lu,Jing Wang,Fulei Tang,Uday P Pratap,Gangadhara R Sareddy,Krishnan M Dhandapani,Ana Capuano,Zoe Arvanitakis,Ratna K Vadlamudi,Darrell W BrannPubMed 35628182
- EXCLI journal 21:656-6792022The diaryl-imidazopyridazine anti-plasmodial compound, MMV652103, exhibits anti-breast cancer activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexis Neumann-Mufweba,Serah Kimani,Saif Feroz Khan,Kelly Chibale,Sharon PrincePubMed 35651652
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BBC3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXH1
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form. |
Species Mouse | Dilution info 1-2 µg/mL | Notes Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. Can be blocked with PUMA peptide (180/193) (ab9644). A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-beta form. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed:11463391, PubMed:23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed:23340338). Regulates ER stress-induced neuronal apoptosis (By similarity).
Alternative names
PUMA, BBC3, JFY-1, p53 up-regulated modulator of apoptosis
Recommended products
Rabbit Polyclonal PUMA antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 129 publications. Immunogen corresponding to Synthetic Peptide within Human BBC3 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BBC3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXH1
- Purification technique
- Affinity purification Immunogen
- Specificity
At least 2 isoforms are known to exist; this antibody will detect both isoforms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PUMA also known as BBC3 (Bcl-2-binding component-3) is a pro-apoptotic protein that plays important mechanical roles in promoting apoptosis. It has a mass of approximately 23 kDa. PUMA is expressed widely in various tissues including the lung liver and heart. The protein works mechanically by interacting with anti-apoptotic Bcl-2 family members releasing pro-apoptotic factors like Bax and Bak which engage mitochondria to trigger cell death.
- Biological function summary
PUMA functions as a pivotal mediator of apoptosis in response to diverse stimuli such as DNA damage and oncogenic stress. As part of the Bcl-2 family PUMA does not typically form a complex with other proteins but functions directly by binding and neutralizing anti-apoptotic Bcl-2 family proteins. This action results in the release of caspase-activating factors from mitochondria essential for programmed cell death.
- Pathways
Several cellular pathways incorporate PUMA to regulate apoptosis. PUMA is notably included in the p53 pathway where p53 transcriptionally activates PUMA following cellular stress or DNA damage. The presence of related proteins like Bax and Bim in the mitochondrial apoptotic pathway links these signals to the mitochondrial death machinery facilitating apoptosis.
- Associated diseases and disorders
PUMA plays significant roles in cancer and neurodegenerative conditions. PUMA overexpression induces excessive cell death relevant in the pathology of neurodegenerative diseases. In cancer PUMA's interactions with proteins like p53 highlight its involvement in tumor suppression suggesting that dysregulation of PUMA expression can contribute to tumorigenesis when anti-apoptotic signals predominate potentially leading to chemoresistance.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-PUMA antibody (ab9643)
PUMA Western blot staining using rabbit Anti-PUMA antibody
Beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL). Incubation time: 1 hour at Room Temperature in 5% NFDM/TBST.
All lanes: Western blot - Anti-PUMA antibody (ab9643) at 2 µg/mL
Lane 1: HEK293 cells were transfected with control siRNAs at 15 µg
Lane 2: HEK293 cells were transfected with PUMA siRNAs at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)
Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9643 at 2 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.
Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (ab9643)
ICC/IF image of ab9643 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9643, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunocytochemistry - Anti-PUMA antibody (ab9643)
Immunocytochemical analysis of K562 cells labeling PUMA with ab9643 at 1 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-PUMA antibody (ab9643)
All lanes: Western blot - Anti-PUMA antibody (ab9643) at 2 µg/mL
Lane 1: K562 cell lysate at 15 µg
Lane 2: NIH3T3 cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 21 kDa
This image is courtesy of an anonymous customer reviewWestern blot - Anti-PUMA antibody (ab9643)
Blocked with 5% milk for 1 hour.
Incubated with the primary antibody for 18 hours.
All lanes: Western blot - Anti-PUMA antibody (ab9643) at 1/1000 dilution
All lanes: HeLa whole cell lysate
SecondaryAll lanes: Alexa Fluor 680-conjugated goat anti-rabbit IgG polyclonal at 1/1 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa, 26 kDa, 42 kDa, 50 kDa
Exposure time: 5s
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