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AB9645

Anti-PUMA antibody

3

(1 Review)

|

(15 Publications)

Rabbit Polyclonal PUMA antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human BBC3 aa 1-50.

View Alternative Names

PUMA, BBC3, JFY-1, p53 up-regulated modulator of apoptosis

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (AB9645)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (AB9645)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling PUMA with ab9645 at 2.5 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (AB9645)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (AB9645)

Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (AB9645)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PUMA antibody (AB9645)

Immunofluorescent analysis of 4% paraformaldehydefixed K562 cells labeling PUMA with ab9645 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing cytosol staining on K562 cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (AB9645)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody (AB9645)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling PUMA with ab9645 at 10 μg/mL. Sections were fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature. Antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western blot - Anti-PUMA antibody (AB9645)
  • WB

Supplier Data

Western blot - Anti-PUMA antibody (AB9645)

Primary incubation : 1 hour at room temperature in 5% NFDM/TBST.

All lanes:

Western blot - Anti-PUMA antibody (ab9645) at 2 µg/mL

All lanes:

K562 cell lysates at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 21 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human BBC3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BXH1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PUMA also known as BBC3 (Bcl-2-binding component-3) is a pro-apoptotic protein that plays important mechanical roles in promoting apoptosis. It has a mass of approximately 23 kDa. PUMA is expressed widely in various tissues including the lung liver and heart. The protein works mechanically by interacting with anti-apoptotic Bcl-2 family members releasing pro-apoptotic factors like Bax and Bak which engage mitochondria to trigger cell death.
Biological function summary

PUMA functions as a pivotal mediator of apoptosis in response to diverse stimuli such as DNA damage and oncogenic stress. As part of the Bcl-2 family PUMA does not typically form a complex with other proteins but functions directly by binding and neutralizing anti-apoptotic Bcl-2 family proteins. This action results in the release of caspase-activating factors from mitochondria essential for programmed cell death.

Pathways

Several cellular pathways incorporate PUMA to regulate apoptosis. PUMA is notably included in the p53 pathway where p53 transcriptionally activates PUMA following cellular stress or DNA damage. The presence of related proteins like Bax and Bim in the mitochondrial apoptotic pathway links these signals to the mitochondrial death machinery facilitating apoptosis.

PUMA plays significant roles in cancer and neurodegenerative conditions. PUMA overexpression induces excessive cell death relevant in the pathology of neurodegenerative diseases. In cancer PUMA's interactions with proteins like p53 highlight its involvement in tumor suppression suggesting that dysregulation of PUMA expression can contribute to tumorigenesis when anti-apoptotic signals predominate potentially leading to chemoresistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis (PubMed : 11463391, PubMed : 23340338). Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis (PubMed : 23340338). Regulates ER stress-induced neuronal apoptosis (By similarity).
See full target information BBC3
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:1173-1187 PubMed40296899

2025

Mycobacterial antigen Ag85B restrains Hodgkin lymphoma tumor growth by inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfeng Cheng,Yiping Shen,Yunfei Zhang,Hailiqiguli Nuriding,Xuemei Wang,Chunyan Fan,Gulibaha Maimaiti,Y U Liu,Yingbin Yue,Danlu Li,Mei Yan

Cell death & disease 15:323 PubMed38724507

2024

Decreased apoptotic priming and loss of BCL-2 dependence are functional hallmarks of Richter's syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Antonella Rigo,Tiziana Vaisitti,Carlo Laudanna,Eleonora Terrabuio,Matilde Micillo,Cristina Frusteri,Beatrice D'Ulivo,Flavia Merigo,Andrea Sbarbati,Kevin Mellert,Peter Möeller,Alessio Montresor,Arianna Di Napoli,Roberto Cirombella,Elena Butturini,Massimo Massaia,Gabriela Constantin,Fabrizio Vinante,Silvia Deaglio,Isacco Ferrarini

International journal of biological sciences 17:2367-2379 PubMed34239363

2021

Recombinant human bone morphogenetic protein 2 and 7 inhibit the degeneration of intervertebral discs by blocking the Puma-dependent apoptotic signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shiwei Xie,Chenyang Zhao,Wei Chen,Gengwu Li,Zhiwei Xiong,Xiangjun Tang,Fan Zhang,Heng Xiao

Cytotechnology 73:63-70 PubMed33505114

2021

Encorafenib enhances TRAIL-induced apoptosis of colorectal cancer cells dependent on p53/PUMA signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenqing Sun,Zhigang Qiu,Bin Ma,Zhengkun Wang

Cell death & disease 11:442 PubMed32513923

2020

Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1.

Applications

Unspecified application

Species

Unspecified reactive species

Li Dong,David L Vaux

Clinical cancer research : an official journal of 26:3371-3383 PubMed32054729

2020

5-Azacitidine Induces NOXA to Prime AML Cells for Venetoclax-Mediated Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Jin,Dan Cojocari,Julie J Purkal,Relja Popovic,Nari N Talaty,Yu Xiao,Larry R Solomon,Erwin R Boghaert,Joel D Leverson,Darren C Phillips

Cell death and differentiation 25:797-808 PubMed29339775

2018

Anti-apoptotic A1 is not essential for lymphoma development in Eµ-Myc mice but helps sustain transplanted Eµ-Myc tumour cells.

Applications

WB

Species

Mouse

Mark Mensink,Natasha S Anstee,Mikara Robati,Robyn L Schenk,Marco J Herold,Suzanne Cory,Cassandra J Vandenberg

Human molecular genetics 20:2807-22 PubMed21531789

2011

Silencing of frataxin gene expression triggers p53-dependent apoptosis in human neuron-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gloria M Palomo,Toñi Cerrato,Ricardo Gargini,Javier Diaz-Nido

Apoptosis : an international journal on programmed cell death 15:71-82 PubMed19943111

2009

Increased expression of the pro-apoptotic Bcl2 family member PUMA and apoptosis by the muscle regulatory transcription factor MyoD in response to a variety of stimuli.

Applications

WB

Species

Mouse

Terri J Harford,Atossa Shaltouki,Crystal M Weyman

British journal of cancer 101:1606-12 PubMed19773755

2009

Mutant p53 mediates survival of breast cancer cells.

Applications

WB

Species

Human

L Y Lim,N Vidnovic,L W Ellisen,C-O Leong
View all publications
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Product promise

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