Anti-PUMA antibody - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUMA antibody - C-terminal (AB227130)

Paraffin-embedded human hepatoma tissue tissue stained for PUMA using ab227130 at 1/500 dilution in immunohistochemical analysis.

• IP

Supplier Data

Immunoprecipitation - Anti-PUMA antibody - C-terminal (AB227130)

PUMA was immunoprecipitated from HCT 116 (human colorectal carcinoma cell line) treated with 30 μM cisplatin for 24 hours, whole cell extract with 4 μg of ab227130. Western blot was performed from the immunoprecipitate using ab227130. Anti-rabbit IgG was used as a secondary reagent.

Lane 1 : Preimmune Rabbit IgG instead of ab227130 in HCT 116 treated with 30 μM cisplatin for 24 hours, whole cell extract.

Lane 2 : ab227130 IP in HCT 116 treated with 30 μM cisplatin for 24 hours, whole cell extract.

15 % SDS-PAGE gel.

All lanes:

Immunoprecipitation - Anti-PUMA antibody - C-terminal (ab227130)

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-PUMA antibody - C-terminal (AB227130)

12 % SDS-PAGE gel.

All lanes:

Western blot - Anti-PUMA antibody - C-terminal (ab227130) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract at 30 µg

Lane 2:

THP-1 (human monocytic leukemia cell line) whole cell extract at 30 µg

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-PUMA antibody - C-terminal (AB227130)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-PUMA antibody - C-terminal (ab227130) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma cell line) with mock treatment for 0 hours, whole cell lysate at 30 µg

Lane 2:

HCT 116 (human colorectal carcinoma cell line) with 30 uM cisplatin treatment for 24 hours, whole cell lysate at 30 µg

Predicted band size: 21 kDa

false