Rabbit Polyclonal Puma gamma/GPR109A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HCAR2.
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- Frontiers in immunology 12:6552122021Hydroxycarboxylic Acid Receptor 2, a Pleiotropically Linked Receptor for the Multiple Sclerosis Drug, Monomethyl Fumarate. Possible Implications for the Inflammatory Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenedetta Parodi,Alessia Sanna,Alessia Cedola,Antonio Uccelli,Nicole Kerlero de RosboPubMed 34084164
- International journal of biological macromolecules 156:1217-12332019An orally administered butyrate-releasing xylan derivative reduces inflammation in dextran sulphate sodium-induced murine colitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengqi Zha,Yang Lv,Huiling Tang,Tingting Li,Yinghua Miao,Junwei Cheng,Guoqing Wang,Yanfang Tan,Yan Zhu,Xiao Xing,Kang Ding,Ying Wang,Hongping YinPubMed 31759015
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:1062-10732018Ketones and pain: unexplored role of hydroxyl carboxylic acid receptor type 2 in the pathophysiology of neuropathic pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSerena Boccella,Francesca Guida,Francesco De Logu,Danilo De Gregorio,Mariacristina Mazzitelli,Carmela Belardo,Monica Iannotta,Nicola Serra,Romina Nassini,Vito de Novellis,Pierangelo Geppetti,Sabatino Maione,Livio LuongoPubMed 30085883
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human HCAR2. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDS4
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40.00000 - 1/300.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Target data
Function
Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.
Alternative names
GPR109A, HCA2, HM74A, NIACR1, HCAR2, Hydroxycarboxylic acid receptor 2, G-protein coupled receptor 109A, G-protein coupled receptor HM74A, Niacin receptor 1, Nicotinic acid receptor
Recommended products
Rabbit Polyclonal Puma gamma/GPR109A antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human HCAR2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human HCAR2. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TDS4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Puma gamma also known as GPR109A is a G-protein-coupled receptor with a molecular weight of approximately 39 kDa. This receptor is expressed in various tissues including adipose tissue the immune system and the gastrointestinal tract. Puma gamma is located on the cell surface where it binds to ligands including niacin and butyrate. Binding leads to changes in cellular activities through signaling specifically influencing cellular responses to external stimuli.
- Biological function summary
GPR109A plays an important role in metabolic processes. This receptor is involved in the regulation of lipolysis in adipocytes thereby controlling fat breakdown. GPR109A is also significant in mediating anti-inflammatory responses. Although it does not form part of a larger complex its signaling affects interactions with other cellular components. These functions contribute to maintaining homeostasis within the body's systems.
- Pathways
GPR109A participates in the cAMP signaling pathway which has importance in energy balance and hormone regulation. When GPR109A activates it inhibits adenylyl cyclase reducing cyclic AMP levels. This receptor is also linked to the anti-inflammatory pathways through interactions with cytokines and other immune-modulating proteins. GPR109A's activity connects closely with proteins such as beta-arrestins and GRK which modulate its signaling efficiency and duration.
- Associated diseases and disorders
GPR109A has associations with obesity and cardiovascular diseases. High levels of GPR109A expression or dysfunction can lead to increased inflammation contributing to metabolic syndrome and related health problems. This receptor's interaction with niacin is therapeutically targeted in managing dyslipidemia a risk factor for cardiovascular diseases. Additionally research indicates potential links between GPR109A and inflammatory bowel disease through modulation of immune cell activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Puma gamma/GPR109A antibody (ab198693)
Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Puma gamma/GPR109A using ab198693 at a 1/40 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Puma gamma/GPR109A antibody (ab198693)
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Puma gamma/GPR109A using ab198693 at a 1/40 dilution.
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