AB89298

Anti-Puma gamma/GPR109A antibody [MM0329-6S24]

Name: Anti-Puma gamma/GPR109A antibody [MM0329-6S24] (ab89298) | Abcam
Brand: Abcam Limited
SKU: AB89298

Be the first to review this product! Submit a review

(2 Publications)

Rat Monoclonal Puma gamma/GPR109A antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HCAR2.

View Alternative Names

GPR109A, HCA2, HM74A, NIACR1, HCAR2, Hydroxycarboxylic acid receptor 2, G-protein coupled receptor 109A, G-protein coupled receptor HM74A, Niacin receptor 1, Nicotinic acid receptor

1 Images

Supplier Data

Western blot - Anti-Puma gamma/GPR109A antibody [MM0329-6S24] (AB89298)

All lanes:

Western blot - Anti-Puma gamma/GPR109A antibody [MM0329-6S24] (ab89298) at 1/500 dilution

All lanes:

Human Placenta tissue lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

MM0329-6S24

Isotype

IgG2

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HCAR2.

Q8TDS4

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

The IgG fraction of culture supernatant was purified by Protein G affinity chromatography.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Puma gamma also known as GPR109A is a G-protein-coupled receptor with a molecular weight of approximately 39 kDa. This receptor is expressed in various tissues including adipose tissue the immune system and the gastrointestinal tract. Puma gamma is located on the cell surface where it binds to ligands including niacin and butyrate. Binding leads to changes in cellular activities through signaling specifically influencing cellular responses to external stimuli.

Biological function summary

GPR109A plays an important role in metabolic processes. This receptor is involved in the regulation of lipolysis in adipocytes thereby controlling fat breakdown. GPR109A is also significant in mediating anti-inflammatory responses. Although it does not form part of a larger complex its signaling affects interactions with other cellular components. These functions contribute to maintaining homeostasis within the body's systems.

Pathways

GPR109A participates in the cAMP signaling pathway which has importance in energy balance and hormone regulation. When GPR109A activates it inhibits adenylyl cyclase reducing cyclic AMP levels. This receptor is also linked to the anti-inflammatory pathways through interactions with cytokines and other immune-modulating proteins. GPR109A's activity connects closely with proteins such as beta-arrestins and GRK which modulate its signaling efficiency and duration.

GPR109A has associations with obesity and cardiovascular diseases. High levels of GPR109A expression or dysfunction can lead to increased inflammation contributing to metabolic syndrome and related health problems. This receptor's interaction with niacin is therapeutically targeted in managing dyslipidemia a risk factor for cardiovascular diseases. Additionally research indicates potential links between GPR109A and inflammatory bowel disease through modulation of immune cell activity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5-methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide.

See full target information HCAR2

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed38201291

2024

A Potent PDK4 Inhibitor for Treatment of Heart Failure with Reduced Ejection Fraction.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichi Aizawa,Akari Ikeda,Shota Tomida,Koki Hino,Yuuki Sugita,Tomoyasu Hirose,Toshiaki Sunazuka,Hiroshi Kido,Shigeyuki Yokoyama,Ryozo Nagai

Molecular nutrition & food research 66:e2200300 PubMed36208084

2022

GPR109a Regulates Phenotypic and Functional Alterations in Macrophages and the Progression of Type 1 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaodi Zhang,Jiahong Li,Ming Zhang,Binbin Li,XiaoHua Pan,Xiaoliang Dong,Li-Long Pan,Jia Sun

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Puma gamma/GPR109A antibody [MM0329-6S24]

Western blot - Anti-Puma gamma/GPR109A antibody [MM0329-6S24] (AB89298)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise