AB3717

Anti-Pumilio 1 antibody

Name: Anti-Pumilio 1 antibody (ab3717) | Abcam
Brand: Abcam Limited
SKU: AB3717
Price: 525 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal Pumilio 1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PUM1.

View Alternative Names

KIAA0099, PUMH1, PUM1, Pumilio homolog 1, HsPUM, Pumilio-1

3 Images

Supplier Data

Immunoprecipitation - Anti-Pumilio 1 antibody (AB3717)

Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 μg/mL.

Lane 1 : rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.

Lane 2 : ab3717 IP in HEK293T whole cell lysate.

Lane 3 : ab3717 IP in HEK293T whole cell lysate

Lane 4 : Control IgG in HEK293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds

All lanes:

Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)

Predicted band size: 126 kDa

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Supplier Data

Western blot - Anti-Pumilio 1 antibody (AB3717)

All lanes:

Western blot - Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/mL

All lanes:

NIH/3T3 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated rabbit anti-goat IgG

Predicted band size: 126 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Pumilio 1 antibody (AB3717)

All lanes:

Western blot - Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/mL

Lane 1:

HEK293T whole cell lysate at 50 µg

Lane 2:

A549 whole cell lysate at 50 µg

Lane 3:

K562 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated rabbit anti-goat IgG

Predicted band size: 126 kDa

false

Exposure time: 10s

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PUM1. The exact immunogen used to generate this antibody is proprietary information.

Q14671

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pumilio 1 also known as PUM1 is a human protein coding for RNA-binding protein involved in the translational regulation of mRNA. It has a molecular weight of approximately 120 kDa. PUM1 expresses in various tissues with higher concentrations in brain placenta and testis. This protein contains a distinct Pumilio Homology Domain which allows it to specifically recognize and bind to a conserved sequence motif known as Pumilio Response Element (PRE) in target mRNAs.

Biological function summary

PUM1 is an important regulator of gene expression. It functions by binding to the 3' untranslated regions of target mRNAs leading to repression of translation and in some cases RNA decay. PUM1 operates within larger ribonucleoprotein complexes collaborating with other regulatory proteins to fine-tune the stability and translation of specific transcripts. This regulation is vital for processes like neuronal development stem cell maintenance and germ cell development.

Pathways

The influence of PUM1 extends to key biological networks such as the cell cycle and long-term potentiation pathways. It interacts with proteins like DDX6 which synergize to mediate post-transcriptional regulation as part of the mRNA decay pathway. Through its role in these pathways PUM1 helps ensure precise control of gene expression necessary for cellular homeostasis and adaptive responses to environmental changes.

The dysfunction of PUM1 is linked to neurological and reproductive conditions. Mutations or alterations in PUM1 expression have associations with spinocerebellar ataxia and gonadal dysgenesis. In these contexts PUM1 aberrations may also involve interactions with proteins such as FMRP known for its roles in neurological development and reproductive processes pointing to a complex network of genetic regulation and disease manifestation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed : 18328718, PubMed : 21397187, PubMed : 21572425, PubMed : 21653694). Mediates post-transcriptional repression of transcripts via different mechanisms : acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed : 22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed : 18776931, PubMed : 20818387, PubMed : 20860814, PubMed : 22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed : 20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed : 22345517, PubMed : 29474920). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed : 26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels : acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed : 25768905, PubMed : 29474920). Plays a role in cytoplasmic sensing of viral infection (PubMed : 25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state : acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (By similarity). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (PubMed : 28431233).

See full target information PUM1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 20:1887-1899 PubMed37857907

2023

RNA molecular recording with an engineered RNA deaminase.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhu Lin,Samentha Kwok,Abigail E Hein,Bao Quoc Thai,Yewande Alabi,Megan S Ostrowski,Ke Wu,Stephen N Floor

Cellular and molecular life sciences : CMLS 76:147-161 PubMed30269240

2018

PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues.

Applications

Unspecified application

Species

Unspecified reactive species

Marcin Sajek,Damian Mikolaj Janecki,Maciej Jerzy Smialek,Barbara Ginter-Matuszewska,Anna Spik,Slawomir Oczkowski,Erkut Ilaslan,Kamila Kusz-Zamelczyk,Maciej Kotecki,Jacek Blazewicz,Jadwiga Jaruzelska

Oncotarget 9:32466-32477 PubMed30197756

2018

SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.

Applications

Unspecified application

Species

Unspecified reactive species

Damian Mikolaj Janecki,Marcin Sajek,Maciej Jerzy Smialek,Maciej Kotecki,Barbara Ginter-Matuszewska,Bogna Kuczynska,Anna Spik,Tomasz Kolanowski,Riko Kitazawa,Maciej Kurpisz,Jadwiga Jaruzelska

The Journal of biological chemistry 286:36492-9 PubMed21865157

2011

Translational regulation of acetylcholinesterase by the RNA-binding protein Pumilio-2 at the neuromuscular synapse.

Applications

IHC-Fr

Species

Mouse

Emilio Marrero,Susana G Rossi,Andrew Darr,Pantelis Tsoulfas,Richard L Rotundo

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Pumilio 1 antibody

Immunoprecipitation - Anti-Pumilio 1 antibody (AB3717)

Western blot - Anti-Pumilio 1 antibody (AB3717)

Western blot - Anti-Pumilio 1 antibody (AB3717)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise