Goat Polyclonal Pumilio 1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PUM1.
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Publications
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- Nature methods 20:1887-18992023RNA molecular recording with an engineered RNA deaminase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizhu Lin,Samentha Kwok,Abigail E Hein,Bao Quoc Thai,Yewande Alabi,Megan S Ostrowski,Ke Wu,Stephen N FloorPubMed 37857907
- Cellular and molecular life sciences : CMLS 76:147-1612018PUM1 and PUM2 exhibit different modes of regulation for SIAH1 that involve cooperativity with NANOS paralogues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarcin Sajek,Damian Mikolaj Janecki,Maciej Jerzy Smialek,Barbara Ginter-Matuszewska,Anna Spik,Slawomir Oczkowski,Erkut Ilaslan,Kamila Kusz-Zamelczyk,Maciej Kotecki,Jacek Blazewicz,Jadwiga JaruzelskaPubMed 30269240
- Oncotarget 9:32466-324772018SPIN1 is a proto-oncogene and SPIN3 is a tumor suppressor in human seminoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDamian Mikolaj Janecki,Marcin Sajek,Maciej Jerzy Smialek,Maciej Kotecki,Barbara Ginter-Matuszewska,Bogna Kuczynska,Anna Spik,Tomasz Kolanowski,Riko Kitazawa,Maciej Kurpisz,Jadwiga JaruzelskaPubMed 30197756
- The Journal of biological chemistry 286:36492-92011Translational regulation of acetylcholinesterase by the RNA-binding protein Pumilio-2 at the neuromuscular synapse.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PUM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14671
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Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted |
| Elephant | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant, Rat, Rabbit | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 - 1/10000 | Notes - |
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cow, Pig, Chimpanzee, Drosophila melanogaster, Rhesus monkey, Gorilla, Orangutan, Elephant, Rat, Rabbit | Dilution info - | Notes - |
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Target data
Function
Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:18328718, PubMed:21397187, PubMed:21572425, PubMed:21653694). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517, PubMed:29474920). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905, PubMed:29474920). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down-regulation at the onset of differentiation (By similarity). Binds specifically to miRNA MIR199A precursor, with PUM2, regulates miRNA MIR199A expression at a postranscriptional level (PubMed:28431233).
Alternative names
KIAA0099, PUMH1, PUM1, Pumilio homolog 1, HsPUM, Pumilio-1
Recommended products
Goat Polyclonal Pumilio 1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PUM1.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PUM1. The exact immunogen used to generate this antibody is proprietary information. Database link Q14671
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pumilio 1 also known as PUM1 is a human protein coding for RNA-binding protein involved in the translational regulation of mRNA. It has a molecular weight of approximately 120 kDa. PUM1 expresses in various tissues with higher concentrations in brain placenta and testis. This protein contains a distinct Pumilio Homology Domain which allows it to specifically recognize and bind to a conserved sequence motif known as Pumilio Response Element (PRE) in target mRNAs.
- Biological function summary
PUM1 is an important regulator of gene expression. It functions by binding to the 3' untranslated regions of target mRNAs leading to repression of translation and in some cases RNA decay. PUM1 operates within larger ribonucleoprotein complexes collaborating with other regulatory proteins to fine-tune the stability and translation of specific transcripts. This regulation is vital for processes like neuronal development stem cell maintenance and germ cell development.
- Pathways
The influence of PUM1 extends to key biological networks such as the cell cycle and long-term potentiation pathways. It interacts with proteins like DDX6 which synergize to mediate post-transcriptional regulation as part of the mRNA decay pathway. Through its role in these pathways PUM1 helps ensure precise control of gene expression necessary for cellular homeostasis and adaptive responses to environmental changes.
- Associated diseases and disorders
The dysfunction of PUM1 is linked to neurological and reproductive conditions. Mutations or alterations in PUM1 expression have associations with spinocerebellar ataxia and gonadal dysgenesis. In these contexts PUM1 aberrations may also involve interactions with proteins such as FMRP known for its roles in neurological development and reproductive processes pointing to a complex network of genetic regulation and disease manifestation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
Pumilio 1 was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3717 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab3717 at 0.1 μg/mL.
Lane 1: rabbit anti-Pumillio 1 antibody IP in HEK293T whole cell lysate.
Lane 2: ab3717 IP in HEK293T whole cell lysate.
Lane 3: ab3717 IP in HEK293T whole cell lysate
Lane 4: Control IgG in HEK293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds
All lanes: Immunoprecipitation - Anti-Pumilio 1 antibody (ab3717)
Predicted band size: 126 kDa
Western blot - Anti-Pumilio 1 antibody (ab3717)
All lanes: Western blot - Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/mL
Lane 1: HEK293T whole cell lysate at 50 µg
Lane 2: A549 whole cell lysate at 50 µg
Lane 3: K562 whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated rabbit anti-goat IgG
Predicted band size: 126 kDa
Exposure time: 10s
Western blot - Anti-Pumilio 1 antibody (ab3717)
All lanes: Western blot - Anti-Pumilio 1 antibody (ab3717) at 0.1 µg/mL
All lanes: NIH/3T3 whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated rabbit anti-goat IgG
Predicted band size: 126 kDa
Exposure time: 10s
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