AB10361

Anti-Pumilio 2 antibody

Name: Anti-Pumilio 2 antibody (ab10361) | Abcam
Brand: Abcam Limited
SKU: AB10361
Price: 545 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal Pumilio 2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PUM2 aa 100-150.

View Alternative Names

KIAA0235, PUMH2, PUM2, Pumilio homolog 2, Pumilio-2

2 Images

Supplier Data

Immunoprecipitation - Anti-Pumilio 2 antibody (AB10361)

Pumilio 2 was immunoprecipitated from HeLa whole cell lysate with 3 μg/mg lysate ab10361. Western blot was performed from the immunoprecipitate using ab10361 at 1 μg/ml dilution.

Lane 1 : ab10361 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

All lanes:

Immunoprecipitation - Anti-Pumilio 2 antibody (ab10361)

Predicted band size: 114 kDa

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Supplier Data

Western blot - Anti-Pumilio 2 antibody (AB10361)

Detection : Chemiluminescence with exposure time of 10 seconds

All lanes:

Western blot - Anti-Pumilio 2 antibody (ab10361) at 1/5000 dilution

Lane 1:

HeLa Whole cell lysate at 5 µg

Lane 2:

HeLa Whole cell lysate at 15 µg

Lane 3:

HeLa Whole cell lysate at 50 µg

Lane 4:

HEK293T Whole cell lysate at 50 µg

Lane 5:

NIH 3T3 Whole cell lysate at 50 µg

Predicted band size: 114 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PUM2 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q8TB72

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/20000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pumilio 2 also known as PUM2 is a member of the PUF family of RNA-binding proteins. It has an approximate mass of 120 kDa. PUM2 is expressed in a variety of tissues including the brain testis and ovary. The protein primarily interacts with the 3'-untranslated regions (3' UTRs) of target mRNAs influencing their stability and translation. PUM2 acts by binding to specific sequences which mediates post-transcriptional regulation of gene expression.

Biological function summary

The regulation of mRNA stability and translation by PUM2 contributes to critical cellular processes such as development and differentiation. PUM2's involvement is observed within the context of RNA-protein complexes where it functions as an important regulator of mRNA fate. Its activity allows cells to tailor protein production according to specific needs such as maintaining stem cell pluripotency or guiding germ cell development.

Pathways

PUM2 participates in the regulation of mRNA translation and degradation aligning with pathways like the RNA processing and cell cycle pathways. In these pathways PUM2 collaborates with elements like the DCP1-DCP2 decapping complex to influence mRNA longevity and turnover. It also interacts with proteins such as NANOS enabling fine-tuned control over cell cycle progression and differentiation.

Altered expression of PUM2 has ties to neurological conditions like intellectual disability and infertility-related disorders. Mutations or misregulation in PUM2 expression can disrupt normal neural function with impacts linked to proteins like FMRP which is associated with fragile X syndrome. In reproductive diseases PUM2 regulation of specific mRNAs influences germline stem cell maintenance connecting its role to fertility issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed : 21397187). Mediates post-transcriptional repression of transcripts via different mechanisms : acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed : 22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed : 18776931, PubMed : 22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed : 22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed : 25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed : 26724866). May regulate DCUN1D3 mRNA levels (PubMed : 25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed : 28431233).

See full target information PUM2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 15: PubMed36073607

2022

Targeting firing rate neuronal homeostasis can prevent seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Fred Mulroe,Wei-Hsiang Lin,Connie Mackenzie-Gray Scott,Najat Aourz,Yuen Ngan Fan,Graham Coutts,R Ryley Parrish,Ilse Smolders,Andrew Trevelyan,Robert C Wykes,Stuart Allan,Sally Freeman,Richard A Baines

Journal of biomedical research 36:255-268 PubMed35965435

2022

RNA binding protein BOULE forms aggregates in mammalian testis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujuan Su,Xinghui Guo,Min Zang,Zhengyao Xie,Tingting Zhao,Eugene Yujun Xu

Cancer discovery 12:1984-2005 PubMed35674408

2022

Stress Granules Determine the Development of Obesity-Associated Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Fonteneau,Alexandra Redding,Hannah Hoag-Lee,Edward S Sim,Stefan Heinrich,Matthias M Gaida,Elda Grabocka

Biomedicine & pharmacotherapy = Biomedecine & phar 114:108772 PubMed30909144

2019

RNA binding protein PUM2 promotes the stemness of breast cancer cells via competitively binding to neuropilin-1 (NRP-1) mRNA with miR-376a.

Applications

Unspecified application

Species

Unspecified reactive species

Lansheng Zhang,Yanwei Chen,Caihong Li,Jinyang Liu,Huiwen Ren,Lishan Li,Xia Zheng,Hui Wang,Zhengxiang Han

Cell structure and function 44:29-39 PubMed30787206

2019

PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyu Wang,Weili Sun,Jiankai Yang,Liang Yang,Chen Li,Hongjiang Liu,Xiaopeng Liu,Baohua Jiao

Molecular cell 73:775-787.e10 PubMed30642763

2019

The RNA-Binding Protein PUM2 Impairs Mitochondrial Dynamics and Mitophagy During Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Davide D'Amico,Adrienne Mottis,Francesca Potenza,Vincenzo Sorrentino,Hao Li,Mario Romani,Vera Lemos,Kristina Schoonjans,Nicola Zamboni,Graham Knott,Bernard L Schneider,Johan Auwerx

Molecular cell 68:808-820.e5 PubMed29129640

2017

The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules.

Applications

Unspecified application

Species

Unspecified reactive species

Anthony Khong,Tyler Matheny,Saumya Jain,Sarah F Mitchell,Joshua R Wheeler,Roy Parker

The Journal of biological chemistry 286:36492-9 PubMed21865157

2011

Translational regulation of acetylcholinesterase by the RNA-binding protein Pumilio-2 at the neuromuscular synapse.

Applications

WB, IHC-Fr

Species

Mouse, Mouse

Emilio Marrero,Susana G Rossi,Andrew Darr,Pantelis Tsoulfas,Richard L Rotundo

Journal of cell science 123:871-80 PubMed20159962

2010

Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells.

Applications

Unspecified application

Species

Unspecified reactive species

Florencia Barrios,Doria Filipponi,Manuela Pellegrini,Maria Paola Paronetto,Sara Di Siena,Raffaele Geremia,Pellegrino Rossi,Massimo De Felici,Emmanuele A Jannini,Susanna Dolci

Proceedings of the National Academy of Sciences of 107:3222-7 PubMed20133610

2010

Mammalian Pumilio 2 regulates dendrite morphogenesis and synaptic function.

Applications

Species

Unspecified reactive species

John P Vessey,Lucia Schoderboeck,Ewald Gingl,Ettore Luzi,Julia Riefler,Francesca Di Leva,Daniela Karra,Sabine Thomas,Michael A Kiebler,Paolo Macchi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pumilio 2 antibody

Immunoprecipitation - Anti-Pumilio 2 antibody (AB10361)

Western blot - Anti-Pumilio 2 antibody (AB10361)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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