AB228743

Anti-Pumilio 2 antibody - N-terminal

Name: Anti-Pumilio 2 antibody - N-terminal (ab228743) | Abcam
Brand: Abcam Limited
SKU: AB228743
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Pumilio 2 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUM2.

View Alternative Names

KIAA0235, PUMH2, PUM2, Pumilio homolog 2, Pumilio-2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 2 antibody - N-terminal (AB228743)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Paraffin-embedded mouse kidney tissue stained for Pumilio 2 with ab228743 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

5% SDS-PAGE

All lanes:

Western blot - Anti-Pumilio 2 antibody - N-terminal (ab228743) at 1/5000 dilution

All lanes:

Human ESC whole cell lysate at 20 µg

Predicted band size: 114 kDa

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Supplier Data

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-Pumilio 2 antibody - N-terminal (ab228743) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Predicted band size: 114 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PUM2. The exact immunogen used to generate this antibody is proprietary information.

Q8TB72

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pumilio 2 also known as PUM2 is a member of the PUF family of RNA-binding proteins. It has an approximate mass of 120 kDa. PUM2 is expressed in a variety of tissues including the brain testis and ovary. The protein primarily interacts with the 3'-untranslated regions (3' UTRs) of target mRNAs influencing their stability and translation. PUM2 acts by binding to specific sequences which mediates post-transcriptional regulation of gene expression.

Biological function summary

The regulation of mRNA stability and translation by PUM2 contributes to critical cellular processes such as development and differentiation. PUM2's involvement is observed within the context of RNA-protein complexes where it functions as an important regulator of mRNA fate. Its activity allows cells to tailor protein production according to specific needs such as maintaining stem cell pluripotency or guiding germ cell development.

Pathways

PUM2 participates in the regulation of mRNA translation and degradation aligning with pathways like the RNA processing and cell cycle pathways. In these pathways PUM2 collaborates with elements like the DCP1-DCP2 decapping complex to influence mRNA longevity and turnover. It also interacts with proteins such as NANOS enabling fine-tuned control over cell cycle progression and differentiation.

Altered expression of PUM2 has ties to neurological conditions like intellectual disability and infertility-related disorders. Mutations or misregulation in PUM2 expression can disrupt normal neural function with impacts linked to proteins like FMRP which is associated with fragile X syndrome. In reproductive diseases PUM2 regulation of specific mRNAs influences germline stem cell maintenance connecting its role to fertility issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (, PubMed : 21397187). Mediates post-transcriptional repression of transcripts via different mechanisms : acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed : 22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed : 18776931, PubMed : 22345517). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed : 22345517). Plays a role in cytoplasmic sensing of viral infection (PubMed : 25340845). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed : 26724866). May regulate DCUN1D3 mRNA levels (PubMed : 25349211). May support proliferation and self-renewal of stem cells. Binds specifically to miRNA MIR199A precursor, with PUM1, regulates miRNA MIR199A expression at a postranscriptional level (PubMed : 28431233).

See full target information PUM2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 14:1234577 PubMed37854606

2023

Single-cell profiling reveals immune disturbances landscape and HLA-F-mediated immune tolerance at the maternal-fetal interface in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Luo,Fulin Liu,Yingzhe Guo,Wenming Xu,Yilin Li,Jun Yi,Thierry Fournier,Séverine Degrelle,Hedia Zitouni,Isabelle Hernandez,Xinghui Liu,Yu Huang,Jun Yue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Pumilio 2 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Western blot - Anti-Pumilio 2 antibody - N-terminal (AB228743)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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