Anti-PURA antibody

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PURA also known as Pur-alpha is a DNA-binding protein involved in the regulation of DNA replication and transcription. It has a molecular mass of about 37-kilodaltons and is widely expressed in various tissues including the brain heart and skeletal muscle. This protein acts at a mechanical level by binding to the purine-rich elements of DNA influencing processes such as the initiation of replication and the transcription of specific genes. PURA interacts with single-stranded DNA as well as RNA highlighting its role in post-transcriptional control.

Biological function summary

PURA is essential for normal cellular activities particularly in neural development and function. It operates not as part of a static structure but engages dynamically with other molecules to exert its effect. PURA can form complexes with proteins such as mRNA binding proteins and helicases allowing it to participate in the modification and stabilization of mRNAs. Through these interactions PURA contributes to neuronal health aiding in processes that govern brain development and synaptic formation.

Pathways

PURA participates in critical biological pathways like cell cycle regulation and RNA transport. Through these pathways PURA interacts with molecules such as retinoblastoma protein and RNA polymerase II playing a pivotal role in ensuring proper cell division and DNA transcription. In the context of RNA transport PURA helps in moving mRNA from the nucleus to the cytoplasm where it regulates protein synthesis essential for cell function and response to external signals.

Associated diseases and disorders

Alterations in PURA function relate closely to neurological disorders like PURA Syndrome and certain cases of intellectual disability. These disorders arise from mutations in the PURA gene which disrupt its interaction network. For instance the disruption can affect its relationship with FMRP another RNA-binding protein linked to Fragile X syndrome. Understanding PURA’s role in these conditions can provide insights into the molecular pathways that when dysregulated result in such neural phenotypes presenting possible avenues for therapeutic interventions.