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AB125200

Anti-PURA antibody

0

(1 Review)

|

(4 Publications)

Rabbit Polyclonal PURA antibody. Suitable for IP and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PURA aa 100-150.

View Alternative Names

PUR1, PURA, Transcriptional activator protein Pur-alpha, Purine-rich single-stranded DNA-binding protein alpha

1 Images
Immunoprecipitation - Anti-PURA antibody (AB125200)
  • IP

Unknown

Immunoprecipitation - Anti-PURA antibody (AB125200)

Detection of PURA by Western Blot of Immunprecipitate. Lane 1; an antibody to PURA at 1µg/ml staining PURA in HeLa whole cell lysate immunoprecipitated using ab125200 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). The lysate in lane 2 is precipitated with an IgG control antibody. Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PURA antibody (ab125200)

Predicted band size: 27 kDa,35 kDa,71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human PURA aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q00577

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab125200 was affinity purified using an epitope specific to PURA immobilized on a solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PURA also known as Pur-alpha is a DNA-binding protein involved in the regulation of DNA replication and transcription. It has a molecular mass of about 37-kilodaltons and is widely expressed in various tissues including the brain heart and skeletal muscle. This protein acts at a mechanical level by binding to the purine-rich elements of DNA influencing processes such as the initiation of replication and the transcription of specific genes. PURA interacts with single-stranded DNA as well as RNA highlighting its role in post-transcriptional control.
Biological function summary

PURA is essential for normal cellular activities particularly in neural development and function. It operates not as part of a static structure but engages dynamically with other molecules to exert its effect. PURA can form complexes with proteins such as mRNA binding proteins and helicases allowing it to participate in the modification and stabilization of mRNAs. Through these interactions PURA contributes to neuronal health aiding in processes that govern brain development and synaptic formation.

Pathways

PURA participates in critical biological pathways like cell cycle regulation and RNA transport. Through these pathways PURA interacts with molecules such as retinoblastoma protein and RNA polymerase II playing a pivotal role in ensuring proper cell division and DNA transcription. In the context of RNA transport PURA helps in moving mRNA from the nucleus to the cytoplasm where it regulates protein synthesis essential for cell function and response to external signals.

Alterations in PURA function relate closely to neurological disorders like PURA Syndrome and certain cases of intellectual disability. These disorders arise from mutations in the PURA gene which disrupt its interaction network. For instance the disruption can affect its relationship with FMRP another RNA-binding protein linked to Fragile X syndrome. Understanding PURA’s role in these conditions can provide insights into the molecular pathways that when dysregulated result in such neural phenotypes presenting possible avenues for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene (PubMed : 1448097, PubMed : 20976240). May play a role in the initiation of DNA replication and in recombination.
See full target information PURA
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:185 PubMed37344459

2023

TRPS1 regulates the opposite effect of progesterone via RANKL in endometrial carcinoma and breast carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Yang,Qiong Fan,Jing Wang,Xiaoming Yang,Jiangjing Yuan,Yuhong Li,Xiao Sun,Yudong Wang

Oncogene 41:4336-4348 PubMed35945453

2022

Forming cytoplasmic stress granules PURα suppresses mRNA translation initiation of IGFBP3 to promote esophageal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lusong Tian,Xiufeng Xie,Urmi Das,Yuling Chen,Yulin Sun,Fang Liu,Haizhen Lu,Peng Nan,Ying Zhu,Xinglu Gu,Haiteng Deng,Jiuyong Xie,Xiaohang Zhao

Nature communications 11:6438 PubMed33353933

2020

MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Kung-Chi Chang,Sarah D Diermeier,Allen T Yu,Lily D Brine,Suzanne Russo,Sonam Bhatia,Habeeb Alsudani,Karen Kostroff,Tawfiqul Bhuiya,Edi Brogi,Darryl J Pappin,C Frank Bennett,Frank Rigo,David L Spector

Genes 11: PubMed33142842

2020

PURα Promotes the Transcriptional Activation of in Oesophageal Squamous Cell Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Sun,Jiajia Gao,Zongpan Jing,Yan Zhao,Yulin Sun,Xiaohang Zhao
View all publications
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