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Rabbit Recombinant Monoclonal PUS10 antibody. Suitable for WB, IP, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-PUS10 antibody [EPR26272-66] (AB313622), expandable thumbnail
  • Western blot - Anti-PUS10 antibody [EPR26272-66] (AB313622), expandable thumbnail
  • Western blot - Anti-PUS10 antibody [EPR26272-66] (AB313622), expandable thumbnail
  • Western blot - Anti-PUS10 antibody [EPR26272-66] (AB313622), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PUS10 antibody [EPR26272-66] (AB313622), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm (PubMed:31819270, PubMed:33023933). In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs (PubMed:30530625, PubMed:31819270, PubMed:33023933). tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs (PubMed:30530625). Does not show tRNA pseudouridylate synthase activity in the nucleus (PubMed:33023933). In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity (PubMed:31819270). Binds pri-miRNAs (PubMed:31819270). Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage (PubMed:14527409, PubMed:19712588). Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death (PubMed:19712588).

Alternative names

CCDC139, DOBI, PUS10, tRNA pseudouridine synthase Pus10, Hup10, Coiled-coil domain-containing protein 139, tRNA pseudouridine 55 synthase, tRNA pseudouridylate synthase, tRNA-uridine isomerase, Psi55 synthase

Recommended products

Rabbit Recombinant Monoclonal PUS10 antibody. Suitable for WB, IP, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26272-66
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein PUS10 also known as pseudouridylate synthase 10 catalyzes the isomerization of uridine to pseudouridine in RNA molecules. This process introduces pseudouridine modifications critical for RNA stability and processing. PUS10 has a molecular mass of approximately 57 kDa and it is expressed in several tissues including the heart and skeletal muscle. This protein is found in both the nucleus and cytoplasm of cells suggesting its role in modifying different types of RNA.

Biological function summary

PUS10 plays significant functions that affect RNA biogenesis and stability. Its involvement in pseudouridylation impacts the structure and function of tRNA rRNA and other small RNA species. This protein is not part of a larger protein complex but works independently. Its activity influences the integrity of RNA molecules which is important for accurate protein synthesis and cellular function.

Pathways

PUS10 significantly contributes to RNA modification and processing pathways. It is a part of the wider RNA maturation process where it cooperates with other RNA-modifying enzymes like dyskerin (DKC1). DKC1 also participates in pseudouridylation highlighting the interconnected roles in ensuring RNA’s proper folding and functionality. PUS10’s involvement in the RNA modification pathway underlines its importance in maintaining RNA function integrity.

Associated diseases and disorders

PUS10 has been linked to several health conditions. It has notable associations with cancer and autoimmune diseases. In cancer alterations in PUS10 expression levels can disrupt normal cell function potentially leading to tumorigenesis. It also interacts with the p53 protein which plays a role in tumor suppression. In autoimmune diseases the misregulation of RNA modifications including pseudouridylation by PUS10 might contribute to autoimmune responses due to altered RNA structures. Understanding PUS10 in these contexts provides insight into how RNA modifications could influence disease progression and treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Immunoprecipitation - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    PUS10 was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab313622 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313622 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: ab313622 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313622 in PC-3 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 24 seconds

    All lanes: Immunoprecipitation - Anti-PUS10 antibody [EPR26272-66] (ab313622) at 1/30 dilution

    All lanes: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Exposure time: 24s

  • Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identity of the lower MW band at approximately 37 kDa is unknown.
    Exposure time: 180 seconds

    All lanes: Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human kidney tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 60 kDa

    Exposure time: 180s

  • Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 180s

  • Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 103 seconds

    All lanes: Western blot - Anti-PUS10 antibody [EPR26272-66] (ab313622) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 3: PC-3 transfected with siRNA specifically targeti PUS10 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 60 kDa

    Exposure time: 103s

  • Flow Cytometry (Intracellular) - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling PUS10 with ab313622 at 1/500 dilution (0.1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular tissue labeling PUS10 with ab313622 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human hepatocellular carcinoma. The section was incubated with ab313622 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinom tissue labeling PUS10 with ab313622 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human colon carcinoma. The section was incubated with ab313622 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody [EPR26272-66] (ab313622), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS10 antibody [EPR26272-66] (ab313622)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PUS10 with ab313622 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human kidney. The section was incubated with ab313622 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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