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Rabbit Recombinant Monoclonal PUS10 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-PUS10 antibody [EPR26272-66] - BSA and Azide free (AB313623), expandable thumbnail
  • Western blot - Anti-PUS10 antibody [EPR26272-66] - BSA and Azide free (AB313623), expandable thumbnail
  • Western blot - Anti-PUS10 antibody [EPR26272-66] - BSA and Azide free (AB313623), expandable thumbnail
  • Western blot - Anti-PUS10 antibody [EPR26272-66] - BSA and Azide free (AB313623), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PUS10 antibody [EPR26272-66] - BSA and Azide free (AB313623), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPFlow Cyt (Intra)IHC-PICC/IF
Human
Tested
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm (PubMed:31819270, PubMed:33023933). In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs (PubMed:30530625, PubMed:31819270, PubMed:33023933). tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs (PubMed:30530625). Does not show tRNA pseudouridylate synthase activity in the nucleus (PubMed:33023933). In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity (PubMed:31819270). Binds pri-miRNAs (PubMed:31819270). Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage (PubMed:14527409, PubMed:19712588). Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death (PubMed:19712588).

Alternative names

CCDC139, DOBI, PUS10, tRNA pseudouridine synthase Pus10, Hup10, Coiled-coil domain-containing protein 139, tRNA pseudouridine 55 synthase, tRNA pseudouridylate synthase, tRNA-uridine isomerase, Psi55 synthase

Recommended products

Rabbit Recombinant Monoclonal PUS10 antibody. Carrier free. Suitable for WB, IP, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26272-66
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein PUS10 also known as pseudouridylate synthase 10 catalyzes the isomerization of uridine to pseudouridine in RNA molecules. This process introduces pseudouridine modifications critical for RNA stability and processing. PUS10 has a molecular mass of approximately 57 kDa and it is expressed in several tissues including the heart and skeletal muscle. This protein is found in both the nucleus and cytoplasm of cells suggesting its role in modifying different types of RNA.

Biological function summary

PUS10 plays significant functions that affect RNA biogenesis and stability. Its involvement in pseudouridylation impacts the structure and function of tRNA rRNA and other small RNA species. This protein is not part of a larger protein complex but works independently. Its activity influences the integrity of RNA molecules which is important for accurate protein synthesis and cellular function.

Pathways

PUS10 significantly contributes to RNA modification and processing pathways. It is a part of the wider RNA maturation process where it cooperates with other RNA-modifying enzymes like dyskerin (DKC1). DKC1 also participates in pseudouridylation highlighting the interconnected roles in ensuring RNA’s proper folding and functionality. PUS10’s involvement in the RNA modification pathway underlines its importance in maintaining RNA function integrity.

Associated diseases and disorders

PUS10 has been linked to several health conditions. It has notable associations with cancer and autoimmune diseases. In cancer alterations in PUS10 expression levels can disrupt normal cell function potentially leading to tumorigenesis. It also interacts with the p53 protein which plays a role in tumor suppression. In autoimmune diseases the misregulation of RNA modifications including pseudouridylation by PUS10 might contribute to autoimmune responses due to altered RNA structures. Understanding PUS10 in these contexts provides insight into how RNA modifications could influence disease progression and treatment.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com