AB211270

Anti-PUS3 antibody - C-terminal

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(3 Publications)

Rabbit Polyclonal PUS3 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PUS3 aa 400 to C-terminus.

View Alternative Names

FKSG32, PUS3, tRNA pseudouridine(38/39) synthase, tRNA pseudouridine synthase 3, tRNA pseudouridylate synthase 3, tRNA-uridine isomerase 3

3 Images

Supplier Data

Immunoprecipitation - Anti-PUS3 antibody - C-terminal (AB211270)

Detection of PUS3 in Immunoprecipitates of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using : 1) ab211270 at 6 μg per reaction. 2) control IgG. For blotting ab211270 was used at 1 μg/ml. Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PUS3 antibody - C-terminal (ab211270)

Predicted band size: 56 kDa

true

Exposure time: 30s

Lab

Western blot - Anti-PUS3 antibody - C-terminal (AB211270)

Western blot : Anti-PUS3 antibody (ab211270) staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab211270 was shown to bind specifically to PUS3. A band was observed at 54-60 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in PUS3 knockout cell line. To generate this image, wild-type and PUS3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PUS3 antibody - C-terminal (ab211270) at 1/500 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

PUS3 knockout HCT 116 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 54-60 kDa

false

Supplier Data

Western blot - Anti-PUS3 antibody - C-terminal (AB211270)

All lanes:

Western blot - Anti-PUS3 antibody - C-terminal (ab211270) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 56 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PUS3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BZE2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab211270 was affinity purified using an epitope specific to PUS3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pseudouridine synthase 3 (PUS3) also known as PUS3 homolog is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. PUS3 has a molecular mass of approximately 54 kDa. This enzyme is primarily located in the nucleoplasm but shows expression in diverse tissues with higher levels observed in the brain and testis. Its role is foundational in modifying RNA substrates impacting the stability and function of RNA.

Biological function summary

PUS3 contributes to essential RNA modifications important for accurate RNA structure and function. It engages in the pseudouridylation of specific RNA molecules including tRNA mRNA and spliceosomal RNAs. PUS3 functions mainly as a monomer and does not typically form part of larger enzyme complexes. The action of PUS3 enhances the stability and translational efficiency of RNA by altering standard nucleosides to pseudouridines influencing the tertiary structure of RNA molecules.

Pathways

PUS3 activity is essential for RNA modification pathways particularly those involving tRNA and mRNA processing. Within these pathways PUS3 cooperates with other pseudouridine synthases and RNA modifying proteins to achieve its biological role. It interacts indirectly with proteins like Dyskerin involved in similar RNA modifications highlighting the network of proteins necessary for proper RNA processing.

PUS3 mutations have been linked to developmental disorders such as intellectual disability. Disruptions in PUS3 lead to improper RNA modification patterns impacting gene expression and neural function. Notably PUS3's altered activity also connects to disorders involving defective RNA maturation where proteins like EXOSC8 part of the RNA exosome share pathways with PUS3 illustrating a relationship between erroneous RNA processing and disease outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.

See full target information PUS3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:2472-2489.e8 PubMed38996458

2024

The molecular basis of tRNA selectivity by human pseudouridine synthase 3.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Lin,Leon Kleemann,Jakub Jeżowski,Dominika Dobosz,Michał Rawski,Paulina Indyka,Grzegorz Ważny,Rahul Mehta,Andrzej Chramiec-Głąbik,Łukasz Koziej,Tristan Ranff,Christian Fufezan,Mateusz Wawro,Jakub Kochan,Joanna Bereta,Sebastian A Leidel,Sebastian Glatt

Evidence-based complementary and alternative medicine : eCAM 2022:9139841 PubMed36337583

2022

"Jing-Ning Granules" Can Alleviate Attention Deficit Hyperactivity Disorder in Rats by Modulating Dopaminergic D2/D1-Like Receptor-Mediated Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ding,Yiyun Ding,Jingjing Wu,Jialin Deng,Qingyang Yu,Junhong Wang

Human mutation 43:2063-2078 PubMed36125428

2022

Destabilization of mutated human PUS3 protein causes intellectual disability.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Yu Lin,Robert Smigiel,Bozena Kuzniewska,Joanna J Chmielewska,Joanna Kosińska,Mateusz Biela,Anna Biela,Anna Kościelniak,Dominika Dobosz,Izabela Laczmanska,Andrzej Chramiec-Głąbik,Jakub Jeżowski,Jakub Nowak,Monika Gos,Sylwia Rzonca-Niewczas,Magdalena Dziembowska,Rafał Ploski,Sebastian Glatt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PUS3 antibody - C-terminal

Immunoprecipitation - Anti-PUS3 antibody - C-terminal (AB211270)

Western blot - Anti-PUS3 antibody - C-terminal (AB211270)

Western blot - Anti-PUS3 antibody - C-terminal (AB211270)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise