Rabbit Polyclonal PUS3 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUS3 aa 400 to C-terminus.
Images
Publications
Filter by
- Human mutation 43:2063-20782022Destabilization of mutated human PUS3 protein causes intellectual disability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Yu Lin,Robert Smigiel,Bozena Kuzniewska,Joanna J Chmielewska,Joanna Kosińska,Mateusz Biela,Anna Biela,Anna Kościelniak,Dominika Dobosz,Izabela Laczmanska,Andrzej Chramiec-Głąbik,Jakub Jeżowski,Jakub Nowak,Monika Gos,Sylwia Rzonca-Niewczas,Magdalena Dziembowska,Rafał Ploski,Sebastian GlattPubMed 36125428
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PUS3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZE2
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Cat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Ferret | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cat, Pig, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Cat, Pig, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Target data
Function
Formation of pseudouridine at position 39 in the anticodon stem and loop of transfer RNAs.
Alternative names
FKSG32, PUS3, tRNA pseudouridine(38/39) synthase, tRNA pseudouridine synthase 3, tRNA pseudouridylate synthase 3, tRNA-uridine isomerase 3
Recommended products
Rabbit Polyclonal PUS3 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUS3 aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PUS3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BZE2
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab211270 was affinity purified using an epitope specific to PUS3 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pseudouridine synthase 3 (PUS3) also known as PUS3 homolog is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. PUS3 has a molecular mass of approximately 54 kDa. This enzyme is primarily located in the nucleoplasm but shows expression in diverse tissues with higher levels observed in the brain and testis. Its role is foundational in modifying RNA substrates impacting the stability and function of RNA.
- Biological function summary
PUS3 contributes to essential RNA modifications important for accurate RNA structure and function. It engages in the pseudouridylation of specific RNA molecules including tRNA mRNA and spliceosomal RNAs. PUS3 functions mainly as a monomer and does not typically form part of larger enzyme complexes. The action of PUS3 enhances the stability and translational efficiency of RNA by altering standard nucleosides to pseudouridines influencing the tertiary structure of RNA molecules.
- Pathways
PUS3 activity is essential for RNA modification pathways particularly those involving tRNA and mRNA processing. Within these pathways PUS3 cooperates with other pseudouridine synthases and RNA modifying proteins to achieve its biological role. It interacts indirectly with proteins like Dyskerin involved in similar RNA modifications highlighting the network of proteins necessary for proper RNA processing.
- Associated diseases and disorders
PUS3 mutations have been linked to developmental disorders such as intellectual disability. Disruptions in PUS3 lead to improper RNA modification patterns impacting gene expression and neural function. Notably PUS3's altered activity also connects to disorders involving defective RNA maturation where proteins like EXOSC8 part of the RNA exosome share pathways with PUS3 illustrating a relationship between erroneous RNA processing and disease outcomes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-PUS3 antibody - C-terminal (ab211270)
All lanes: Western blot - Anti-PUS3 antibody - C-terminal (ab211270) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30s
Immunoprecipitation - Anti-PUS3 antibody - C-terminal (ab211270)
Detection of PUS3 in Immunoprecipitates of 293T whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using: 1) ab211270 at 6 μg per reaction. 2) control IgG. For blotting ab211270 was used at 1 μg/ml. Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-PUS3 antibody - C-terminal (ab211270)
Developed using the ECL technique.
Predicted band size: 56 kDa
Exposure time: 30s
Western blot - Anti-PUS3 antibody - C-terminal (ab211270)
Western blot: Anti-PUS3 antibody (ab211270) staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab211270 was shown to bind specifically to PUS3. A band was observed at 54-60 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in PUS3 knockout cell line. To generate this image, wild-type and PUS3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-PUS3 antibody - C-terminal (ab211270) at 1/500 dilution
Lane 1: Wild-type HCT 116 cell lysate at 20 µg
Lane 2: PUS3 knockout HCT 116 cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: MCF7 cell lysate at 20 µg
SecondaryLanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution
Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 54-60 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com