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Rabbit Polyclonal PUS7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PZ0
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Rat
Expected
Tested
Expected
Cow
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Human

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Species

Rat

Dilution info

0.04000-0.40000 µg/mL

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow

Dilution info

-

Notes

-

Target data

Function

Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141, PubMed:30778726). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).

Alternative names

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Rabbit Polyclonal PUS7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PZ0
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

PUS7 participates in the post-transcriptional modification of RNA which is critical for RNA maturation and function. This enzyme does not work in isolation; it interacts with other components of the RNA modification machinery. PUS7's activity enhances the structural stability of RNA molecules and affects the efficiency of protein synthesis by modifying RNA substrates fine-tuning their roles in processes like translation and splicing.

Activity summary

Pseudouridine synthase 7 (PUS7) also known as PUS7L or pseudouridylate synthase 7 is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. It has a molecular weight of approximately 80 kDa. This protein is highly conserved and widely expressed in various cell types including in tissues like the brain liver and testes. PUS7 is present in the nucleus where it modifies specific uridines in rRNA tRNA and snRNA impacting their stability and function.

Pathways

PUS7 is involved in the RNA modification and processing pathways specifically those related to ribosome biogenesis and mRNA splicing. These pathways are central to the synthesis and maturation of various RNA species ensuring proper protein production. PUS7 interacts closely with proteins like fibrillarin in the nucleolus during rRNA processing and U2 auxiliary factor in mRNA splicing aligning with transcription dynamics and ribosome assembly.

Associated diseases and disorders

Aberrant PUS7 function associates with certain neurodevelopmental disorders and cancers. Alterations in PUS7 activity can disrupt normal RNA processing leading to defects in brain development and function notably in conditions such as intellectual disability. The enzyme's altered function also relates to cancer where dysregulated RNA modification contributes to tumorigenesis. Proteins connected with these disorders like dyskerin and U2AF65 interact with PUS7 highlighting its involvement in disease mechanisms.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human endometrium sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Western blot - Anti-PUS7 antibody (ab224119)

    All lanes: Western blot - Anti-PUS7 antibody (AB224119) at 0.4 µg/mL

    All lanes: K562 cell lysates

    Predicted band size: 75 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PUS7 antibody (ab224119)

    PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PUS7 (green) using ab224119 (4 μg/ml) in ICC/IF.

  • Western blot - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Western blot - Anti-PUS7 antibody (ab224119)

    All lanes: Western blot - Anti-PUS7 antibody (AB224119) at 1/100 dilution

    Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate

    Lane 2: NBT-II (Rat Wistar bladder tumor cell line) cell lysate

    Predicted band size: 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human testis sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human cervix uterine sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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