Rabbit Polyclonal PUS7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus.
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Publications
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- Journal of cardiothoracic surgery 18:2222023Higher expression of pseudouridine synthase 7 promotes non-small cell lung cancer progression and suggests a poor prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihong Zhang,Yongde Zhu,Yonghuang Tan,Biao Chen,Shichao Shan,Gengyu Zhang,Jianjun LuPubMed 37420297
- Journal of experimental & clinical cancer research : CR 40:1702021HSP90-dependent PUS7 overexpression facilitates the metastasis of colorectal cancer cells by regulating LASP1 abundance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Song,Ming Guo,Shuai Xu,Xiaotian Song,Bin Bai,Zhengyan Li,Jie Chen,Yanxin An,Yongzhan Nie,Kaichun Wu,Shiqi Wang,Qingchuan ZhaoPubMed 33990203
- Digestive diseases and sciences 67:1260-12702021Pseudouridylate Synthase 7 Promotes Cell Proliferation and Invasion in Colon Cancer Through Activating PI3K/AKT/mTOR Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiming Du,Aimin Gong,Xuefeng Zhao,Guixin WangPubMed 33811565
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PZ0
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141, PubMed:30778726, PubMed:31477916, PubMed:34718722, PubMed:35051350). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919, PubMed:31477916, PubMed:35051350). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed:35051350). Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed:35051350). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).
Alternative names
KIAA1897, PUS7, Pseudouridylate synthase 7 homolog
Recommended products
Rabbit Polyclonal PUS7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PUS7 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PZ0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pseudouridine synthase 7 (PUS7) also known as PUS7L or pseudouridylate synthase 7 is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. It has a molecular weight of approximately 80 kDa. This protein is highly conserved and widely expressed in various cell types including in tissues like the brain liver and testes. PUS7 is present in the nucleus where it modifies specific uridines in rRNA tRNA and snRNA impacting their stability and function.
- Biological function summary
PUS7 participates in the post-transcriptional modification of RNA which is critical for RNA maturation and function. This enzyme does not work in isolation; it interacts with other components of the RNA modification machinery. PUS7's activity enhances the structural stability of RNA molecules and affects the efficiency of protein synthesis by modifying RNA substrates fine-tuning their roles in processes like translation and splicing.
- Pathways
PUS7 is involved in the RNA modification and processing pathways specifically those related to ribosome biogenesis and mRNA splicing. These pathways are central to the synthesis and maturation of various RNA species ensuring proper protein production. PUS7 interacts closely with proteins like fibrillarin in the nucleolus during rRNA processing and U2 auxiliary factor in mRNA splicing aligning with transcription dynamics and ribosome assembly.
- Associated diseases and disorders
Aberrant PUS7 function associates with certain neurodevelopmental disorders and cancers. Alterations in PUS7 activity can disrupt normal RNA processing leading to defects in brain development and function notably in conditions such as intellectual disability. The enzyme's altered function also relates to cancer where dysregulated RNA modification contributes to tumorigenesis. Proteins connected with these disorders like dyskerin and U2AF65 interact with PUS7 highlighting its involvement in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human endometrium sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-PUS7 antibody (ab224119)
All lanes: Western blot - Anti-PUS7 antibody (ab224119) at 0.4 µg/mL
All lanes: K562 cell lysates
Predicted band size: 75 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PUS7 antibody (ab224119)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PUS7 (green) using ab224119 (4 μg/ml) in ICC/IF.
Western blot - Anti-PUS7 antibody (ab224119)
All lanes: Western blot - Anti-PUS7 antibody (ab224119) at 1/100 dilution
Lane 1: NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2: NBT-II (Rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 75 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human testis sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human cervix uterine sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PUS7 antibody (ab224119)
Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human pancreas sections labeling PUS7 antibody with ab224119 at 1/200. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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