AB226245

Anti-PUS7 antibody

Name: Anti-PUS7 antibody (ab226245) | Abcam
Brand: Abcam Limited
SKU: AB226245
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal PUS7 antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PUS7 aa 200-300.

View Alternative Names

KIAA1897, PUS7, Pseudouridylate synthase 7 homolog

1 Images

Supplier Data

Immunoprecipitation - Anti-PUS7 antibody (AB226245)

PUS7 was immunoprecipitated from HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226245 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226245 at 1 μg/ml.

Lane 1 : ab226245 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PUS7 antibody (ab226245)

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PUS7 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q96PZ0

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226245 was affinity purified using an epitope specific to PUS7 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pseudouridine synthase 7 (PUS7) also known as PUS7L or pseudouridylate synthase 7 is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. It has a molecular weight of approximately 80 kDa. This protein is highly conserved and widely expressed in various cell types including in tissues like the brain liver and testes. PUS7 is present in the nucleus where it modifies specific uridines in rRNA tRNA and snRNA impacting their stability and function.

Biological function summary

PUS7 participates in the post-transcriptional modification of RNA which is critical for RNA maturation and function. This enzyme does not work in isolation; it interacts with other components of the RNA modification machinery. PUS7's activity enhances the structural stability of RNA molecules and affects the efficiency of protein synthesis by modifying RNA substrates fine-tuning their roles in processes like translation and splicing.

Pathways

PUS7 is involved in the RNA modification and processing pathways specifically those related to ribosome biogenesis and mRNA splicing. These pathways are central to the synthesis and maturation of various RNA species ensuring proper protein production. PUS7 interacts closely with proteins like fibrillarin in the nucleolus during rRNA processing and U2 auxiliary factor in mRNA splicing aligning with transcription dynamics and ribosome assembly.

Aberrant PUS7 function associates with certain neurodevelopmental disorders and cancers. Alterations in PUS7 activity can disrupt normal RNA processing leading to defects in brain development and function notably in conditions such as intellectual disability. The enzyme's altered function also relates to cancer where dysregulated RNA modification contributes to tumorigenesis. Proteins connected with these disorders like dyskerin and U2AF65 interact with PUS7 highlighting its involvement in disease mechanisms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed : 28073919, PubMed : 29628141, PubMed : 30778726, PubMed : 31477916, PubMed : 34718722, PubMed : 35051350). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs) : pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed : 29628141). Also catalyzes pseudouridylation of mRNAs : mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed : 28073919, PubMed : 31477916, PubMed : 35051350). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed : 35051350). Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed : 35051350). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed : 29628141).

See full target information PUS7

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:5567-5580 PubMed38517371

2024

CRIF1 attenuates doxorubicin-mediated mitochondrial dysfunction and myocardial senescence via regulating PXDN.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Zhou,Guilan Zhai,Ge Tian

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PUS7 antibody

Immunoprecipitation - Anti-PUS7 antibody (AB226245)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise