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AB226257

Anti-PUS7 antibody

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(3 Publications)

Rabbit Polyclonal PUS7 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PUS7 aa 100-150.

View Alternative Names

KIAA1897, PUS7, Pseudouridylate synthase 7 homolog

1 Images
Western blot - Anti-PUS7 antibody (AB226257)
  • WB

Supplier Data

Western blot - Anti-PUS7 antibody (AB226257)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PUS7 antibody (ab226257) at 0.04 µg

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 75 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PUS7 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q96PZ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab226257 was affinity purified using an epitope specific to PUS7 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pseudouridine synthase 7 (PUS7) also known as PUS7L or pseudouridylate synthase 7 is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. It has a molecular weight of approximately 80 kDa. This protein is highly conserved and widely expressed in various cell types including in tissues like the brain liver and testes. PUS7 is present in the nucleus where it modifies specific uridines in rRNA tRNA and snRNA impacting their stability and function.
Biological function summary

PUS7 participates in the post-transcriptional modification of RNA which is critical for RNA maturation and function. This enzyme does not work in isolation; it interacts with other components of the RNA modification machinery. PUS7's activity enhances the structural stability of RNA molecules and affects the efficiency of protein synthesis by modifying RNA substrates fine-tuning their roles in processes like translation and splicing.

Pathways

PUS7 is involved in the RNA modification and processing pathways specifically those related to ribosome biogenesis and mRNA splicing. These pathways are central to the synthesis and maturation of various RNA species ensuring proper protein production. PUS7 interacts closely with proteins like fibrillarin in the nucleolus during rRNA processing and U2 auxiliary factor in mRNA splicing aligning with transcription dynamics and ribosome assembly.

Aberrant PUS7 function associates with certain neurodevelopmental disorders and cancers. Alterations in PUS7 activity can disrupt normal RNA processing leading to defects in brain development and function notably in conditions such as intellectual disability. The enzyme's altered function also relates to cancer where dysregulated RNA modification contributes to tumorigenesis. Proteins connected with these disorders like dyskerin and U2AF65 interact with PUS7 highlighting its involvement in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed : 28073919, PubMed : 29628141, PubMed : 30778726, PubMed : 31477916, PubMed : 34718722, PubMed : 35051350). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs) : pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed : 29628141). Also catalyzes pseudouridylation of mRNAs : mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed : 28073919, PubMed : 31477916, PubMed : 35051350). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed : 35051350). Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed : 35051350). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed : 29628141).
See full target information PUS7
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of thoracic disease 16:7819-7835 PubMed39678883

2024

Prognostic value and potential biological function of in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Xi,Jing Zeng,Yundong Zhou,Weiyu Shen,Hirokazu Taniguchi,Retnagowri Rajandram,Sivakumar Krishnasamy

Nature methods 21:2024-2033 PubMed39349603

2024

Absolute quantitative and base-resolution sequencing reveals comprehensive landscape of pseudouridine across the human transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Haiqi Xu,Linzhen Kong,Jingfei Cheng,Khatoun Al Moussawi,Xiufei Chen,Aleema Iqbal,Peter A C Wing,James M Harris,Senko Tsukuda,Azman Embarc-Buh,Guifeng Wei,Alfredo Castello,Skirmantas Kriaucionis,Jane A McKeating,Xin Lu,Chun-Xiao Song

RNA (New York, N.Y.) 27:1400-1411 PubMed34376564

2021

Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique.

Applications

Unspecified application

Species

Unspecified reactive species

Cecilia Martinez Campos,Kevin Tsai,David G Courtney,Hal P Bogerd,Christopher L Holley,Bryan R Cullen
View all publications
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