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AB325665

Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric)

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Chicken Monoclonal antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Pva, Pvalb, Parvalbumin alpha

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (AB325665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (AB325665)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells and neurons of the molecular layer with ab325665 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (AB325665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (AB325665)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of Purkinje cells and neurons of the molecular layer with ab325665 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 400X.

Western blot - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (AB325665)
  • WB

Supplier Data

Western blot - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (AB325665)

Western blotting of rat whole brain homogenate with ab325665 (10 µg/ml) and detected with anti-chicken-HRP. The antibody recognizes endogenous Pvalb in the rat whole brain homogenate at 12 kDa.

All lanes:

Western blot - Anti-Pvalb antibody [L114/81] - Chicken IgY (Chimeric) (ab325665) at 10 µg/mL

All lanes:

rat whole brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 12 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

L114/81

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

No cross-reactivity reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L114/81 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L114/81 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
See full target information Pvalb
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Product promise

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For full details, please see our Terms & Conditions

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