Rabbit Polyclonal PVL LukS antibody. Suitable for WB and reacts with Staphylococcus aureus samples. Cited in 4 publications.
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- Antimicrobial agents and chemotherapy 66:e00240222022Hinokiflavone Attenuates the Virulence of Methicillin-Resistant Staphylococcus aureus by Targeting Caseinolytic Protease P.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangri Kong,Bingmei Wang,Xiaoyu Chen,Li Wang,Xingye Wang,Juan Hou,Lin Wei,Liyan Sui,Chi Zhang,Jiyu Guan,Yanhe Luan,Wei Wang,Wu Song,Yicheng ZhaoPubMed 35862746
- Virulence 13:578-5882022Nepetin reduces virulence factors expression by targeting ClpP against MRSA-induced pneumonia infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShisong Jing,Xinran Ren,Li Wang,Xiangri Kong,Xingye Wang,Xiren Chang,Xuerui Guo,Yan Shi,Jiyu Guan,Tiedong Wang,Bingmei Wang,Wu Song,Yicheng ZhaoPubMed 35363605
- Microbiology spectrum 10:e02340212022Quercetin Reduces the Virulence of S. aureus by Targeting ClpP to Protect Mice from MRSA-Induced Lethal Pneumonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShisong Jing,Xiangri Kong,Li Wang,Heming Wang,Jiaxuan Feng,Lin Wei,Ying Meng,Chang Liu,Xiren Chang,Yishen Qu,Jiyu Guan,Haimiao Yang,Chi Zhang,Yicheng Zhao,Wu SongPubMed 35319277
- International journal of molecular sciences 21:2020Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrian Jun Chu et. al.PubMed 32796751
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| WB | |
|---|---|
| Staphylococcus aureus | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Staphylococcus aureus | Dilution info - | Notes If probing against purified protein, at least 10 ng of pure toxin can be detected with 0.5 ug/ml of the primary antibody combined with a suitable dilution of a secondary anti-rabbit antibody |
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Rabbit Polyclonal PVL LukS antibody. Suitable for WB and reacts with Staphylococcus aureus samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PVL LukS also known as Panton-Valentine leukocidin S component is a part of a bacterial toxin produced by certain strains of Staphylococcus aureus. It has a molecular mass of approximately 32.9 kDa. This protein is expressed mainly in pathogenic strains of Staphylococcus aureus making it an important factor for assessing bacterial virulence. PVL LukS works closely with another component LukF and together they create pores in the membranes of host immune cells especially leukocytes causing cell lysis.
- Biological function summary
PVL LukS forms a binary toxin complex by pairing with the LukF component. This complexly binds to specific receptors on the surface of human immune cells. Once bound the two components oligomerize to form a transmembrane pore. The formation of these pores disrupts cell membrane integrity leading to cell death and tissue damage which contributes significantly to the pathogenesis of the infection caused by Staphylococcus aureus.
- Pathways
PVL LukS participates mainly in immune cell evasion strategies of the bacteria. It plays a role in pathways that disrupt host immune function by targeting and destroying leukocytes which are significant players in the body's defense mechanism. Its interaction with the protein LukF is integral as their combined action enables the formation of the apoptogenic complex on host cell surfaces. This pathway is a part of the virulence strategy employed by Staphylococcus aureus to induce local infection and evade the immune response.
- Associated diseases and disorders
The PVL LukS protein is associated with severe skin and soft tissue infections as well as necrotizing pneumonia. These conditions arise due to the lytic action of the PVL toxin on immune cells weakening the host's defense and allowing the bacteria to spread. The target is connected to other virulence factors of Staphylococcus aureus such as protein A which interferes with host immune recognition thereby compounding the severity of infections associated with PVL.
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Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-PVL LukS antibody (ab190473)
All lanes: Western blot - Anti-PVL LukS antibody (ab190473) at 0.5 µg/mL
All lanes: Purified PVL Luk-S
SecondaryAll lanes: Goat anti rabbit-AP at 1/3000 dilution
Predicted band size: 35 kDa
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