Rabbit Recombinant Monoclonal PVRIG/CD112R antibody. Suitable for Flow Cyt and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IP | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding.
C7orf15, PVRIG, Transmembrane protein PVRIG, CD112 receptor, Poliovirus receptor-related immunoglobulin domain-containing protein, CD112R
Rabbit Recombinant Monoclonal PVRIG/CD112R antibody. Suitable for Flow Cyt and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR23797-104
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
The PVRIG also known as CD112R is a cell surface receptor involved in immune responses. It has a molecular mass of approximately 38 kDa. Expressed mainly in immune cells including T cells and natural killer (NK) cells PVRIG binds with high affinity to its ligand CD112. This interaction modulates immune cell function by transmitting inhibitory signals that downregulate immune activation serving as a potential immune checkpoint.
PVRIG impacts immune cell behavior and is part of the inhibitory receptor family. Its primary role is to fine-tune the immune response by decreasing overactivation helping to maintain immune homeostasis. PVRIG interacts with other immune inhibitory receptors like TIGIT and PD-1 forming a complex network that suppresses overactive T cell responses. This process is important for preventing autoimmune reactions while allowing normal immune surveillance.
PVRIG participates in immune checkpoint pathways that influence T cell regulation. It plays a role in the immune checkpoint receptor pathway which is pivotal for controlling immune tolerance and immune-mediated inflammation. Within these pathways PVRIG works closely with other receptors like PD-1 forming a blockade that maintains the balance of immune activation and inhibition and participates in the immune synapse during antigen presentation.
PVRIG is relevant to cancer and autoimmune diseases. Its expression on T cells can be upregulated in tumor environments contributing to immune escape in cancers like melanoma. The PVRIG-CD112 interaction competes with other pathways such as TIGIT and CD112 further impacting tumor immune evasion. In autoimmune diseases PVRIG may prevent excessive immune responses linking it to conditions like rheumatoid arthritis where altered immune regulation occurs.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged PVRIG/CD112R construct cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with rabbit IgG and ab256808, then fixed with 2% PFA followed by intracellularly staining with anti-myc tag conjugated to AlexaFluor®647.
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab256808. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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