Rabbit Recombinant Monoclonal PVRL1/NECTIN1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-PVRL1/NECTIN1 antibody [EPR24275-38] - BSA and Azide free (Capture) (AB278331), expandable thumbnail](https://content.abcam.com/products/images/pvrl1-nectin1-antibody-epr24275-38-bsa-and-azide-free-capture-ab278331--sandwich-elisa-img168726.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-PVRL1/NECTIN1 antibody [EPR24275-38] - BSA and Azide free (Capture) (AB278331)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system (PubMed:21325282). Acts by forming homophilic or heterophilic trans-dimers (PubMed:21325282). Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4 (By similarity). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Involved in synaptogegesis (By similarity). Has some neurite outgrowth-promoting activity (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN3: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Required for enamel mineralization (By similarity). (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV (PubMed:21980294, PubMed:25231300, PubMed:28381567, PubMed:28542478, PubMed:34587223, PubMed:38857290, PubMed:39048823, PubMed:39048830, PubMed:7721102, PubMed:9616127, PubMed:9657005). Constitutes the major receptor for herpes simplex virus 1/HHV-1 entry into host cells (PubMed:34587223).
Alternative names
CD111, HVEC, PRR1, PVRL1, NECTIN1, Nectin-1, Herpes virus entry mediator C, Herpesvirus Ig-like receptor, Nectin cell adhesion molecule 1, Poliovirus receptor-related protein 1, Herpesvirus entry mediator C, HveC, HIgR
Recommended products
Rabbit Recombinant Monoclonal PVRL1/NECTIN1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24275-38
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab278331 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human NECTIN1. The recommended pair for sandwich ELISA is:
Capture: ab278331, Human NECTIN1 Capture Antibody (unconjugated)
Detector: Anti-PVRL1/NECTIN1 antibody - BSA and Azide free (Detector) ab278333, Human NECTIN1 Detector Antibody (unconjugated)
The reference range value is 156 - 10000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PVRL1 also known as NECTIN1 or nectin-1 functions mechanically as an immunoglobulin-like cell adhesion molecule. It plays a role in creating adherens junctions between cells operating as both a homophilic and heterophilic binding partner. Nectin-1 has a molecular mass of approximately 97 kDa. It is expressed prominently in epithelial tissues and particularly in the nervous system which suggests it plays an important role in cell-cell communication in these areas.
- Biological function summary
Nectin-1 facilitates cellular processes through its involvement in cell adhesion complexes. It functions as part of a larger complex including proteins like afadin which links to the actin cytoskeleton and promotes cell junction integrity. This organization impacts cellular adhesion migration and proliferation. Nectin-1's adhesive functions are fundamental in maintaining structural tissues and transmitting signals within cells a necessity for development and tissue maintenance.
- Pathways
Nectin-1 contributes to the activation of the MAPK/ERK signaling pathway central to regulating various cell functions including proliferation and differentiation. It also participates in the cadherin-catenin complex pathway working in conjunction with E-cadherin a critical protein in maintaining epithelial cell layer integrity. These pathways highlight nectin-1’s role as a signal transducer affecting how cells interact and react to their environment.
- Associated diseases and disorders
Nectin-1 is implicated in cleft lip and palate disorder. Mutations affecting nectin-1 can disrupt cell adhesion processes necessary for proper development of facial structures. Furthermore alterations in nectin-1 gene expression are associated with susceptibility to herpes simplex virus infections as nectin-1 acts as a viral entry receptor. In these conditions its interaction with closely related membrane proteins such as nectin-3 is significant in disease manifestation and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-PVRL1/NECTIN1 antibody [EPR24275-38] - BSA and Azide free (Capture) (ab278331), expandable thumbnail](https://content.abcam.com/products/images/pvrl1-nectin1-antibody-epr24275-38-bsa-and-azide-free-capture-ab278331--sandwich-elisa-img168726.jpg "Sandwich ELISA - Anti-PVRL1/NECTIN1 antibody [EPR24275-38] - BSA and Azide free (Capture) (ab278331)")
Sandwich ELISA - Anti-PVRL1/NECTIN1 antibody [EPR24275-38] - BSA and Azide free (Capture) (ab278331)
Example of Human NECTIN1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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