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Rabbit Recombinant Monoclonal PVRL1/NECTIN1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (AB323705), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (AB323705), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (AB323705), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (AB323705), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (AB323705), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Mouse
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system (PubMed:21325282). Acts by forming homophilic or heterophilic trans-dimers (PubMed:21325282). Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4 (By similarity). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Involved in synaptogegesis (By similarity). Has some neurite outgrowth-promoting activity (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN3: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Required for enamel mineralization (By similarity). (Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV (PubMed:21980294, PubMed:25231300, PubMed:28381567, PubMed:28542478, PubMed:34587223, PubMed:38857290, PubMed:39048823, PubMed:39048830, PubMed:7721102, PubMed:9616127, PubMed:9657005). Constitutes the major receptor for herpes simplex virus 1/HHV-1 entry into host cells (PubMed:34587223).

Additional Targets

Nectin1

Alternative names

CD111, HVEC, PRR1, PVRL1, NECTIN1, Nectin-1, Herpes virus entry mediator C, Herpesvirus Ig-like receptor, Nectin cell adhesion molecule 1, Poliovirus receptor-related protein 1, Herpesvirus entry mediator C, HveC, HIgR

Rabbit Recombinant Monoclonal PVRL1/NECTIN1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR29038-520
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab323705 is the carrier-free version of Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling PVRL1/NECTIN1 with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse cardiac muscle.

    The section was incubated with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling PVRL1/NECTIN1 with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human cardiac muscle.

    The section was incubated with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PVRL1/NECTIN1 with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse cerebrum.

    The section was incubated with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 for 10 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling PVRL1/NECTIN1 with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human lung cancer.

    The section was incubated with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PVRL1/NECTIN1 with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human tonsil.

    The section was incubated with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PVRL1/NECTIN1 with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 at 1/2000 (0.252 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human cerebrum.

    The section was incubated with Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Western blot - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lanes 1-4 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross reactivity with human IgG at 1/2000 and lanes 5-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000

    Low expression: testis, heart (PMID: 11090177), liver.

    The identity of the bands lower than 57 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] (Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704) at 1/1000 dilution

    Lane 1: Untreated human hippocampus tissue lysate at 20 µg

    Lane 2: Human hippocampus tissue lysate treated with Protein Deglycosylation MIX II at 20 µg

    Lane 3: Human liver tissue lysate at 20 µg

    Lane 4: Human testis tissue lysate at 20 µg

    Lane 5: Mouse brain tissue lysate at 20 µg

    Lane 6: Mouse heart tissue lysate at 20 µg

    Lane 7: Mouse hippocampus lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 5 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 57,75 kDa, 75 kDa, 36 kDa

    Exposure time: 3s

  • Western blot - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705), expandable thumbnail

    Western blot - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] - BSA and Azide free (ab323705)

    This data was developed using Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: A20.

    The identity of the bands lower than 57 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 37 seconds; Lanes 3-5: 6 seconds.

    All lanes: Western blot - Anti-PVRL1/NECTIN1 antibody [EPR29038-520] (Anti-PVRL1/NECTIN1 antibody [EPR29038-520] ab323704) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting PVRL1 whole cell lysate at 20 µg

    Lane 3: HaCaT (human skin keratinocyte) whole cell lysate at 20 µg

    Lane 4: A20 (mouse reticulum sarcoma b lymphocyte) whole cell lysate at 20 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 57,75 kDa, 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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