AB190794

Anti-PWP1 antibody [EPR16130]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PWP1 antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Periodic tryptophan protein 1 homolog, Keratinocyte protein IEF SSP 9502, PWP1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PWP1 antibody [EPR16130] (AB190794)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PWP1 antibody [EPR16130] (AB190794)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human pancreas tissue labeling PWP1 with ab190794 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PWP1 antibody [EPR16130] (AB190794)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PWP1 antibody [EPR16130] (AB190794)

Intracellular Flow Cytometry analysis of PWP1 expression in Jurkat cells using ab190794 at 1/30 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

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Western blot - Anti-PWP1 antibody [EPR16130] (AB190794)

All lanes:

Western blot - Anti-PWP1 antibody [EPR16130] (ab190794) at 1/10000 dilution

All lanes:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 79 kDa

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Supplier Data

Western blot - Anti-PWP1 antibody [EPR16130] (AB190794)

All lanes:

Western blot - Anti-PWP1 antibody [EPR16130] (ab190794) at 1/1000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16130

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PWP1 protein also known as Periodic tryptophan protein 1 homolog is a 90 kDa nuclear protein that participates in the assembly of ribosomal subunits. It is predominantly expressed in the nucleolus and is part of the WD40 protein family characterized by repeating units that form a β-propeller structure. This structure facilitates its interaction with various molecular partners allowing it to play a role in ribosome biogenesis.

Biological function summary

PWP1 is involved in the nucleolar function and ribosome production. It acts as part of a multiprotein complex that assists in the maturation of pre-ribosomal particles. The protein contributes to the processing and assembly of ribosomal RNA ensuring proper ribonucleoprotein configuration. This function is especially important in regulating gene expression by controlling protein synthesis at the ribosomal level.

Pathways

PWP1 is a component of the ribosome biogenesis pathway which integrates its activity with other proteins such as NOP58 and RRP9. It is also linked to the RNA processing pathway where it interacts with other nucleolar proteins to ensure accurate RNA transcription and modification. These pathways are essential for maintaining cellular homeostasis and responding to growth signals impacting cell proliferation.

PWP1's dysfunction has connections with cancer and disorders relating to ribosomal dysregulation. Aberrant expression or mutation of PWP1 may lead to disruptions in ribosomal biogenesis contributing to oncogenesis. The protein is associated with abnormal regulatory networks alongside proteins like NPM1 which also plays a role in tumorigenesis. Investigations into PWP1 offer insights into potential biomarkers and targets for therapeutic intervention in ribosome-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chromatin-associated factor that regulates transcription (PubMed : 29065309). Regulates Pol I-mediated rRNA biogenesis and, probably, Pol III-mediated transcription (PubMed : 29065309). Regulates the epigenetic status of rDNA (PubMed : 29065309).

See full target information PWP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biology open 7: PubMed30361412

2018

PWP1 promotes nutrient-responsive expression of 5S ribosomal RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Rita Cerejeira Matos,Tapio I Heino,Ville Hietakangas

Developmental cell 43:240-252.e5 PubMed29065309

2017

PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar Regulation of Ribosomal Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Jaakko Mattila,Sami Ventelä,Leena Yadav,Wei Zhang,Nicole Lamichane,Jari Sundström,Otto Kauko,Reidar Grénman,Markku Varjosalo,Jukka Westermarck,Ville Hietakangas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PWP1 antibody [EPR16130]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PWP1 antibody [EPR16130] (AB190794)

Flow Cytometry (Intracellular) - Anti-PWP1 antibody [EPR16130] (AB190794)

Western blot - Anti-PWP1 antibody [EPR16130] (AB190794)

Western blot - Anti-PWP1 antibody [EPR16130] (AB190794)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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