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AB251038

Anti-PWP1 antibody [EPR16131] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PWP1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Periodic tryptophan protein 1 homolog, Keratinocyte protein IEF SSP 9502, PWP1

3 Images
Flow Cytometry (Intracellular) - Anti-PWP1 antibody [EPR16131] - BSA and Azide free (AB251038)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PWP1 antibody [EPR16131] - BSA and Azide free (AB251038)

This data was developed using ab190795, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of PWP1 expression in Jurkat cells using ab190795 at 1/180 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody.

Western blot - Anti-PWP1 antibody [EPR16131] - BSA and Azide free (AB251038)
  • WB

Supplier Data

Western blot - Anti-PWP1 antibody [EPR16131] - BSA and Azide free (AB251038)

This data was developed using ab190795, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (<a href='/en-us/products/primary-antibodies/pwp1-antibody-epr16131-c-terminal-ab190795'>ab190795</a>) at 1/10000 dilution

Lane 1:

293 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Hela cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 79 kDa

false

Western blot - Anti-PWP1 antibody [EPR16131] - BSA and Azide free (AB251038)
  • WB

Supplier Data

Western blot - Anti-PWP1 antibody [EPR16131] - BSA and Azide free (AB251038)

This data was developed using ab190795, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PWP1 antibody [EPR16131] - C-terminal (<a href='/en-us/products/primary-antibodies/pwp1-antibody-epr16131-c-terminal-ab190795'>ab190795</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

NIH-3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

Observed band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16131

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab251038 is the carrier-free version of ab190795.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PWP1 protein also known as Periodic tryptophan protein 1 homolog is a 90 kDa nuclear protein that participates in the assembly of ribosomal subunits. It is predominantly expressed in the nucleolus and is part of the WD40 protein family characterized by repeating units that form a β-propeller structure. This structure facilitates its interaction with various molecular partners allowing it to play a role in ribosome biogenesis.
Biological function summary

PWP1 is involved in the nucleolar function and ribosome production. It acts as part of a multiprotein complex that assists in the maturation of pre-ribosomal particles. The protein contributes to the processing and assembly of ribosomal RNA ensuring proper ribonucleoprotein configuration. This function is especially important in regulating gene expression by controlling protein synthesis at the ribosomal level.

Pathways

PWP1 is a component of the ribosome biogenesis pathway which integrates its activity with other proteins such as NOP58 and RRP9. It is also linked to the RNA processing pathway where it interacts with other nucleolar proteins to ensure accurate RNA transcription and modification. These pathways are essential for maintaining cellular homeostasis and responding to growth signals impacting cell proliferation.

PWP1's dysfunction has connections with cancer and disorders relating to ribosomal dysregulation. Aberrant expression or mutation of PWP1 may lead to disruptions in ribosomal biogenesis contributing to oncogenesis. The protein is associated with abnormal regulatory networks alongside proteins like NPM1 which also plays a role in tumorigenesis. Investigations into PWP1 offer insights into potential biomarkers and targets for therapeutic intervention in ribosome-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin-associated factor that regulates transcription (PubMed : 29065309). Regulates Pol I-mediated rRNA biogenesis and, probably, Pol III-mediated transcription (PubMed : 29065309). Regulates the epigenetic status of rDNA (PubMed : 29065309).
See full target information PWP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 21:454-462 PubMed31746378

2019

Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqing Hu,Wenyue Wang,Qing Ma,Tao Liu,Jiefeng Zhang,Jicun Zhang
View all publications
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Product promise

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