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AB230514

Anti-PXK antibody

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(1 Publication)

Rabbit Polyclonal PXK antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Pxk aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PX domain-containing protein kinase-like protein, MONaKA, PXK

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PXK antibody (AB230514)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PXK antibody (AB230514)

Formalin-fixed, paraffin-embedded mouse brain cortex tissue stained for PXK using ab230514 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PXK antibody (AB230514)
  • WB

Supplier Data

Western blot - Anti-PXK antibody (AB230514)

All lanes:

Western blot - Anti-PXK antibody (ab230514) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Predicted band size: 65 kDa

false

Western blot - Anti-PXK antibody (AB230514)
  • WB

Supplier Data

Western blot - Anti-PXK antibody (AB230514)

All lanes:

Western blot - Anti-PXK antibody (ab230514) at 1/1000 dilution

All lanes:

Mouse brain lysate at 35 µg

Predicted band size: 65 kDa

false

Western blot - Anti-PXK antibody (AB230514)
  • WB

Supplier Data

Western blot - Anti-PXK antibody (AB230514)

All lanes:

Western blot - Anti-PXK antibody (ab230514) at 1/1000 dilution

Lane 1:

Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate at 2 µg

Lane 2:

HEK-293 cell lysate transiently transfected with the PXK gene at 2 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Pxk aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q8BX57

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PXK also known as Phosphoinositide-binding protein FAM20-kinase or MOYBG Protein Kinase-like is a membrane-associated protein with a molecular mass of approximately 59 kDa. Experts have identified it for its kinase-like properties although it lacks conventional kinase activity. PXK is expressed in various tissues including brain liver and kidney with significant presence in neuronal cells. Its mechanical role involves modulating neuronal signaling and membrane dynamics through interactions with phosphoinositides.
Biological function summary

PXK participates in the regulation of cellular organization and synaptic functions. While not a direct component of signaling complexes it influences processes related to cytoskeletal rearrangement and membrane trafficking. Through its phosphoinositide-binding domains PXK impacts the localization and assembly of proteins involved in synaptic vesicle turnover. As a scaffolding protein it indirectly affects signal transduction pathways by altering the spatial arrangement of interacting proteins.

Pathways

PXK influences the phosphoinositide signaling pathway and the synaptic vesicle cycle. Its interactions with proteins like dynamin and synaptojanin allow researchers to deduce its role in vesicle endocytosis and membrane recycling. PXK indirectly modulates the activity of these proteins impacting the efficiency of synaptic transmission. These pathways underline its role in maintaining synaptic stability and plasticity essential for proper neuronal communication.

Researchers have linked PXK to neurodegenerative diseases and certain immune disorders. Alterations in PXK expression have association with conditions such as Alzheimer’s disease where disrupted synaptic function is an important feature. Furthermore its interaction with immune-related proteins like NF-κB suggests a role in autoimmune processes. These links highlight the importance of PXK in maintaining cellular function and its potential as a therapeutic target in related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds to and modulates brain Na,K-ATPase subunits ATP1B1 and ATP1B3 and may thereby participate in the regulation of electrical excitability and synaptic transmission. May not display kinase activity.
See full target information PXK
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 32:1623-1635 PubMed39308508

2024

Exosomal microRNA let-7c-5p enhances cell malignant characteristics by inhibiting TAGLN in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Y I Li,Tianyi Wang,Haoran Ding,Shiyong Zhuang,Xiaobo Dai,Bing Yan
View all publications
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Product promise

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