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AB118336

Anti-PXR antibody

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(5 Publications)

Mouse Polyclonal PXR antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NR1I2.

View Alternative Names

PXR, NR1I2, Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X receptor, Steroid and xenobiotic receptor, SXR

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PXR antibody (AB118336)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PXR antibody (AB118336)

ab118336 at 10 μg/ml dilution staining PXR in HepG2 cells by Immunofluorescence.

Western blot - Anti-PXR antibody (AB118336)
  • WB

Unknown

Western blot - Anti-PXR antibody (AB118336)

All lanes:

Western blot - Anti-PXR antibody (ab118336) at 1 µg/mL

Lane 1:

PXR transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 50 kDa

true

Western blot - Anti-PXR antibody (AB118336)
  • WB

Unknown

Western blot - Anti-PXR antibody (AB118336)

All lanes:

Western blot - Anti-PXR antibody (ab118336) at 1 µg/mL

All lanes:

Rat liver tissue lysate at 50 µg

Predicted band size: 50 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NR1I2.

O75469

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PXR also known as the pregnane X receptor or NR1I2 is a nuclear receptor involved in the regulation of gene expression. It plays a significant role in detoxification processes. PXR has a molecular mass of approximately 49 kDa. This receptor is primarily expressed in the liver and intestine where it mediates the induction of enzymes important in metabolizing xenobiotics and endobiotics. Notably PXR activation leads to the expression of cytochrome P450 enzymes including CYP3A4 which are essential in drug metabolism.
Biological function summary

The pregnane X receptor is an important sensor in the body's defense system against potentially harmful compounds. PXR forms a heterodimer complex with the retinoid X receptor (RXR) to exert its function. It binds to response elements in the promoter regions of target genes to regulate their transcription. This receptor is highly responsive to a variety of ligands including medications like buy tiagabine and endogenous steroids such as the pregnanolone chemical structure. Ligands binding to PXR result in the transcriptional upregulation of genes involved in several metabolic pathways.

Pathways

The pregnane X receptor has significant roles in the regulation of drug metabolism and disposition pathways. PXR activation leads to the induction of detoxifying enzymes and transporters prominently within the xenobiotic metabolism pathway. It is closely linked with CYP450 enzymes particularly CYP3A4 and the transport protein MDR1. These proteins work together to increase the metabolism and clearance of a variety of drugs and toxins thereby protecting the organism from potential harm.

PXR is associated with conditions involving drug-induced liver injury and inflammatory bowel disease. Its regulation of CYP3A4 impacts the metabolism of various drugs which can lead to altered efficacy or toxicity as seen in these conditions. Additionally PXR dysregulation has implications in liver diseases where nuclear receptors are critical for maintaining metabolic homeostasis. Interactions between PXR and other nuclear receptors like the constitutive androstane receptor (CAR) further demonstrate its involvement in hepatic health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
See full target information NR1I2
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Chemical & pharmaceutical bulletin 71:334-341 PubMed36858593

2023

Anti-tumor Effect of Gambogenic Acid and Its Effect on CYP2C and CYP3A after Oral Administration.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Sun,Mengdi Pang,Xiaozhu Tang,Qianqian Xu,Daiyin Peng,Weidong Chen

Heliyon 6:e05357 PubMed33163657

2020

PXR is a target of (-)-epicatechin in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel Ortiz-Flores,Andrés Portilla-Martínez,Francisco Cabrera-Pérez,Nayelli Nájera,Eduardo Meaney,Francisco Villarreal,Javier Pérez-Durán,Guillermo Ceballos

Molecular medicine reports 20:771-778 PubMed31180561

2019

Effect of copper nanoparticles on brain cytochrome P450 enzymes in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Wang,Huaqiao Tang,Min Xu,Jie Luo,Ling Zhao,Fei Shi,Gang Ye,Cheng Lv,Yinglun Li

Biomedical microdevices 20:54 PubMed29946898

2018

Study of melatonin-mediated effects on various hepatic inflammatory responses stimulated by IL-6 in a new HepG2-on-a-chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Jang,Andreas Manz,Thomas Volk,Astrid Kleber

The Journal of pharmacology and experimental thera 366:332-340 PubMed29891588

2018

Berberine Upregulates P-Glycoprotein in Human Caco-2 Cells and in an Experimental Model of Colitis in the Rat via Activation of Nrf2-Dependent Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Wanghui Jing,Yasaman Safarpour,Ting Zhang,Pengqi Guo,Guoning Chen,Xiaoming Wu,Qiang Fu,Yitao Wang
View all publications
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