AB192579

Anti-PXR antibody

Name: Anti-PXR antibody (ab192579) | Abcam
Brand: Abcam Limited
SKU: AB192579
Price: 535 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(21 Publications)

Rabbit Polyclonal PXR antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NR1I2.

View Alternative Names

PXR, NR1I2, Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X receptor, Steroid and xenobiotic receptor, SXR

1 Images

Unknown

Western blot - Anti-PXR antibody (AB192579)

All lanes:

Western blot - Anti-PXR antibody (ab192579) at 1/500 dilution

Lane 1:

Mouse liver

Lane 2:

Rat liver

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NR1I2.

O75469

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192579 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PXR also known as the pregnane X receptor or NR1I2 is a nuclear receptor involved in the regulation of gene expression. It plays a significant role in detoxification processes. PXR has a molecular mass of approximately 49 kDa. This receptor is primarily expressed in the liver and intestine where it mediates the induction of enzymes important in metabolizing xenobiotics and endobiotics. Notably PXR activation leads to the expression of cytochrome P450 enzymes including CYP3A4 which are essential in drug metabolism.

Biological function summary

The pregnane X receptor is an important sensor in the body's defense system against potentially harmful compounds. PXR forms a heterodimer complex with the retinoid X receptor (RXR) to exert its function. It binds to response elements in the promoter regions of target genes to regulate their transcription. This receptor is highly responsive to a variety of ligands including medications like buy tiagabine and endogenous steroids such as the pregnanolone chemical structure. Ligands binding to PXR result in the transcriptional upregulation of genes involved in several metabolic pathways.

Pathways

The pregnane X receptor has significant roles in the regulation of drug metabolism and disposition pathways. PXR activation leads to the induction of detoxifying enzymes and transporters prominently within the xenobiotic metabolism pathway. It is closely linked with CYP450 enzymes particularly CYP3A4 and the transport protein MDR1. These proteins work together to increase the metabolism and clearance of a variety of drugs and toxins thereby protecting the organism from potential harm.

PXR is associated with conditions involving drug-induced liver injury and inflammatory bowel disease. Its regulation of CYP3A4 impacts the metabolism of various drugs which can lead to altered efficacy or toxicity as seen in these conditions. Additionally PXR dysregulation has implications in liver diseases where nuclear receptors are critical for maintaining metabolic homeostasis. Interactions between PXR and other nuclear receptors like the constitutive androstane receptor (CAR) further demonstrate its involvement in hepatic health and disease.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.

See full target information NR1I2

style

left-column

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 15:366 PubMed41053031

2025

TGR5 dysfunction underlies chronic social defeat stress via cAMP/PKA signaling pathway in the hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Chen,Qinji Zhou,Yong He,Yue Wang,Yanyi Jiang,Yi Ren,Yikun Ren,Junchao Cai,Heming Yu,Chong Chen,Ke Cheng,Peng Xie

British journal of pharmacology 182:1508-1527 PubMed39667762

2024

Atractylodes macrocephala Koidz polysaccharide ameliorates DSS-induced colitis in mice by regulating the gut microbiota and tryptophan metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Wen Zhang,Meng-Jiao Yang,Chun-Yu Liao,Reham Taha,Qing-Yu Li,Mohammed Ismail Abdelmotalab,Si-Yu Zhao,Yan Xu,Zhen-Zhou Jiang,Cheng-Han Chu,Xin Huang,Chun-Hua Jiao,Li-Xin Sun

Pharmaceutics 15: PubMed37631299

2023

Effect of Gut Microbiota on the Pharmacokinetics of Nifedipine in Spontaneously Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zhou,Haijun Yang,Peng Zhu,Yujie Liu,Yanjuan Zhang,Wei Zhang,Honghao Zhou,Xiong Li,Qing Li

Acta pharmacologica Sinica 44:2075-2090 PubMed37344564

2023

Pregnane X receptor activation alleviates renal fibrosis in mice via interacting with p53 and inhibiting the Wnt7a/β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hua Ming,Zhi-Lin Luan,Yao Yao,Hang-Chi Liu,Shu-Yuan Hu,Chun-Xiu Du,Cong Zhang,Yi-Hang Zhao,Ying-Zhi Huang,Xiao-Wan Sun,Rong-Fang Qiao,Hu Xu,You-Fei Guan,Xiao-Yan Zhang

CNS neuroscience & therapeutics 29:3460-3478 PubMed37269088

2023

Pregnane X receptor (PXR) deficiency protects against spinal cord injury by activating NRF2/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Na Xuan,Zhen-Xin Hu,Zhen-Fu Jiang,Cong Zhang,Xiao-Wan Sun,Wen-Hua Ming,Hui-Tao Liu,Rong-Fang Qiao,Lin-Jie Shen,Shao-Bo Liu,Guan-Yu Wang,Lin Wen,Zhi-Lin Luan,Jian Yin

Drug metabolism and disposition: the biological fate of chemicals 50:1464-1471 PubMed36184081

2022

Chronic Treatment With WY-14643 Induces Tumorigenesis and Triglyceride Accumulation in Mouse Livers.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Shicheng Fan,Yifei Zhang,Min Huang,Yue Gao,Huichang Bi

Nutrients 14: PubMed35889800

2022

Protective Effect of Fermented Loose Dark Tea and Particle on MAPK and PXR/AhR Signaling Pathways Induced by Electronic Cigarette Exposure in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Xu,Yufei Zhou,Lijun Yu,Xiangxiang Huang,Jianan Huang,Kunbo Wang,Zhonghua Liu

Cell death & disease 13:359 PubMed35436984

2022

B-cell lymphoma 6 alleviates nonalcoholic fatty liver disease in mice through suppression of fatty acid transporter CD36.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Zhang,Yue Li,Chao Zhang,Kun Huang,Jing Zhao,Sheng Le,Lang Jiang,Hao Liu,Peiwen Yang,Xiaoyue Xiao,Jizhang Yu,Jie Wu,Ping Ye,Jiahong Xia

Cells 11: PubMed35053380

2022

Phosphorylation-Induced Ubiquitination and Degradation of PXR through CDK2-TRIM21 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyao Qin,Yu Xin,Yong Bian,Xuan Yang,Tao Xi,Jing Xiong

International journal of molecular sciences 22: PubMed34299071

2021

Liver Bile Acid Changes in Mouse Models of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Harpreet Kaur,Drew Seeger,Svetlana Golovko,Mikhail Golovko,Colin Kelly Combs

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PXR antibody

Western blot - Anti-PXR antibody (AB192579)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise