AB217375

Anti-PXR antibody

Name: Anti-PXR antibody (ab217375) | Abcam
Brand: Abcam Limited
SKU: AB217375
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PXR antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NR1I2 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

PXR, NR1I2, Nuclear receptor subfamily 1 group I member 2, Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X receptor, Steroid and xenobiotic receptor, SXR

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PXR antibody (AB217375)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PXR antibody (AB217375)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Mouse small intestine tissue labeling PXR with ab217375 at a 1/400 dilution, followed by a conjugated secondary and DAB staining.

Lab

Western blot - Anti-PXR antibody (AB217375)

All lanes:

Western blot - Anti-PXR antibody (ab217375) at 1/300 dilution

All lanes:

Liver lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human NR1I2 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O75469

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PXR also known as the pregnane X receptor or NR1I2 is a nuclear receptor involved in the regulation of gene expression. It plays a significant role in detoxification processes. PXR has a molecular mass of approximately 49 kDa. This receptor is primarily expressed in the liver and intestine where it mediates the induction of enzymes important in metabolizing xenobiotics and endobiotics. Notably PXR activation leads to the expression of cytochrome P450 enzymes including CYP3A4 which are essential in drug metabolism.

Biological function summary

The pregnane X receptor is an important sensor in the body's defense system against potentially harmful compounds. PXR forms a heterodimer complex with the retinoid X receptor (RXR) to exert its function. It binds to response elements in the promoter regions of target genes to regulate their transcription. This receptor is highly responsive to a variety of ligands including medications like buy tiagabine and endogenous steroids such as the pregnanolone chemical structure. Ligands binding to PXR result in the transcriptional upregulation of genes involved in several metabolic pathways.

Pathways

The pregnane X receptor has significant roles in the regulation of drug metabolism and disposition pathways. PXR activation leads to the induction of detoxifying enzymes and transporters prominently within the xenobiotic metabolism pathway. It is closely linked with CYP450 enzymes particularly CYP3A4 and the transport protein MDR1. These proteins work together to increase the metabolism and clearance of a variety of drugs and toxins thereby protecting the organism from potential harm.

PXR is associated with conditions involving drug-induced liver injury and inflammatory bowel disease. Its regulation of CYP3A4 impacts the metabolism of various drugs which can lead to altered efficacy or toxicity as seen in these conditions. Additionally PXR dysregulation has implications in liver diseases where nuclear receptors are critical for maintaining metabolic homeostasis. Interactions between PXR and other nuclear receptors like the constitutive androstane receptor (CAR) further demonstrate its involvement in hepatic health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.

See full target information NR1I2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Toxicology research 11:235-244 PubMed35237428

2022

Ameliorative impacts of chrysin against gibberellic acid-induced liver and kidney damage through the regulation of antioxidants, oxidative stress, inflammatory cytokines, and apoptosis biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Mohamed Soliman,Adil Aldhahrani,Ahmed Gaber,Walaa F Alsanie,Wafaa Abdou Mohamed,Mohamed M M Metwally,Mohamed Elbadawy,Mustafa Shukry

Journal of medicinal chemistry 63:3701-3712 PubMed32160459

2020

Garcinoic Acid Is a Natural and Selective Agonist of Pregnane X Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Desirée Bartolini,Francesca De Franco,Pierangelo Torquato,Rita Marinelli,Bruno Cerra,Riccardo Ronchetti,Arne Schon,Francesca Fallarino,Antonella De Luca,Guido Bellezza,Ivana Ferri,Angelo Sidoni,William G Walton,Samuel J Pellock,Matthew R Redinbo,Sridhar Mani,Roberto Pellicciari,Antimo Gioiello,Francesco Galli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PXR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PXR antibody (AB217375)

Western blot - Anti-PXR antibody (AB217375)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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