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AB102601

Anti-PYCR1 antibody

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(7 Publications)

Rabbit Polyclonal PYCR1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 1-300.

View Alternative Names

P5C reductase 1, P5CR 1, PYCR1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody (AB102601)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody (AB102601)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling PYCR1 with ab102601 at 1/250 dilution.
Antigen Retrieval : EDTA based buffer, pH 8.0, 15min.

Immunocytochemistry/ Immunofluorescence - Anti-PYCR1 antibody (AB102601)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PYCR1 antibody (AB102601)

Immunofluorescence analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell labeling PYCR1 protein at mitochondria (green) with ab102601 at 1/500 dilution. Blue : Hoechst 33342 staining.

Western blot - Anti-PYCR1 antibody (AB102601)
  • WB

Unknown

Western blot - Anti-PYCR1 antibody (AB102601)

12% SDS PAGE

All lanes:

Western blot - Anti-PYCR1 antibody (ab102601) at 1/1000 dilution

All lanes:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P32322

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCR1 also known as Pyrroline-5-carboxylate reductase 1 is an enzyme with a molecular weight of approximately 33 kDa. It facilitates the conversion of pyrroline-5-carboxylate to proline an essential amino acid. PYCR1 is mainly expressed in human tissues with high metabolic activity such as the liver kidney and pancreas which highlights its role in amino acid metabolism. This enzyme plays a central role in maintaining the balance between cellular oxidation and reduction and is typically active in the cytoplasm of the cells.
Biological function summary

PYCR1 participates in the proline biosynthesis pathway and contributes to the cellular response to stress. It functions as a homopolymer indicating its need to form complexes for activity. The production of proline is important as proline protects cells by maintaining membrane integrity and stabilizing proteins during environmental stress like oxidative stress. Proline synthesis involving PYCR1 is essential for collagen production which supports tissue and structural integrity in humans.

Pathways

PYCR1 acts within the proline biosynthesis pathway and the larger metabolism of amino acids. This enzyme works closely with ALDH4A1 which is another enzyme that catalyzes the earlier stages of the proline biosynthesis pathway before PYCR1 performs its function. The pathway plays a significant role in plant and animal cell adaptation to environmental changes. By modulating these pathways cells gain enhanced abilities to manage osmotic pressures and support vital functions under stress conditions linking PYCR1's activity to cellular survival and resilience.

PYCR1 mutations or dysfunction is associated with Cutis laxa a rare connective tissue disorder characterized by loose sagging skin and systemic involvement. These dysfunctions affect proline synthesis impairing collagen production which leads to abnormal connective tissue structure. PYCR1 is also connected to pyrroline-5-carboxylate synthetase implicating dysfunction in the larger metabolic framework that supports protein synthesis and cellular health. These disturbances underline the enzyme's importance in maintaining normal physiological conditions and hint at possible therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 16730026, PubMed : 19648921, PubMed : 23024808, PubMed : 28258219). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 16730026, PubMed : 23024808). Involved in the cellular response to oxidative stress (PubMed : 16730026, PubMed : 19648921).
See full target information Pyrroline-5-carboxylate reductase 1, mitochondrial
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 74:290 PubMed40848149

2025

The transcription factor FOXA1 upregulates PYCR1-mediated autophagy to suppress antitumor immunity in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Liu,Xiaoyan Yang,Xuemei Tang,Bo Tang

Cancer biology & therapy 26:2546219 PubMed40808274

2025

Hypoxia-induced PYCR1 regulates glycolysis and histone lactylation to promote bladder cancer progression and metastasis via SLC6A14/Glutamine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Li,Qinghua Jiang,Quan Yang,Yujie Zhou,Jiansong Wang

Histology and histopathology 40:89-100 PubMed38826151

2024

PYCR1 expresses in cancer-associated fibroblasts and accelerates the progression of C6 glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mingkun Zhang,Baibin Bi,Guangcun Liu,Xiaoyong Fan

International journal of oncology 63: PubMed37293856

2023

Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Li,Ying Jiang,Jian Liu,Huifeng Fu,Quan Yang,Wei Song,Yuanwei Li

Theranostics 13:2896-2913 PubMed37284449

2023

Cisplatin attenuates taste cell homeostasis and induces inflammatory activation in the circumvallate papilla.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Ren,Xudong Cha,Rui Xu,Tianyu Wang,Caiquan Liang,Janice Chou,Xiujuan Zhang,Fengzhen Li,Shenglei Wang,Boyu Cai,Peihua Jiang,Hong Wang,Huanhai Liu,Yiqun Yu

Biochemical and biophysical research communications 550:99-106 PubMed33706104

2021

MiR-328-3p inhibits lung adenocarcinoma-genesis by downregulation PYCR1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancong Lu,Junhong Lin,Yu Zhou,Kai Ye,Changquan Fang

The Journal of clinical investigation 124:398-412 PubMed24316975

2013

MYC-driven accumulation of 2-hydroxyglutarate is associated with breast cancer prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Terunuma,Nagireddy Putluri,Prachi Mishra,Ewy A Mathé,Tiffany H Dorsey,Ming Yi,Tiffany A Wallace,Haleem J Issaq,Ming Zhou,J Keith Killian,Holly S Stevenson,Edward D Karoly,King Chan,Susmita Samanta,DaRue Prieto,Tiffany Y T Hsu,Sarah J Kurley,Vasanta Putluri,Rajni Sonavane,Daniel C Edelman,Jacob Wulff,Adrienne M Starks,Yinmeng Yang,Rick A Kittles,Harry G Yfantis,Dong H Lee,Olga B Ioffe,Rachel Schiff,Robert M Stephens,Paul S Meltzer,Timothy D Veenstra,Thomas F Westbrook,Arun Sreekumar,Stefan Ambs
View all publications
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Product promise

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