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AB206693

Anti-PYCR1 antibody

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(2 Publications)

Rabbit Polyclonal PYCR1 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 250 to C-terminus.

View Alternative Names

P5C reductase 1, P5CR 1, PYCR1

1 Images
Western blot - Anti-PYCR1 antibody (AB206693)
  • WB

Supplier Data

Western blot - Anti-PYCR1 antibody (AB206693)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PYCR1 antibody (ab206693) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 33 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P32322

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab206693 was affinity purified using an epitope specific to PYCR1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCR1 also known as Pyrroline-5-carboxylate reductase 1 is an enzyme with a molecular weight of approximately 33 kDa. It facilitates the conversion of pyrroline-5-carboxylate to proline an essential amino acid. PYCR1 is mainly expressed in human tissues with high metabolic activity such as the liver kidney and pancreas which highlights its role in amino acid metabolism. This enzyme plays a central role in maintaining the balance between cellular oxidation and reduction and is typically active in the cytoplasm of the cells.
Biological function summary

PYCR1 participates in the proline biosynthesis pathway and contributes to the cellular response to stress. It functions as a homopolymer indicating its need to form complexes for activity. The production of proline is important as proline protects cells by maintaining membrane integrity and stabilizing proteins during environmental stress like oxidative stress. Proline synthesis involving PYCR1 is essential for collagen production which supports tissue and structural integrity in humans.

Pathways

PYCR1 acts within the proline biosynthesis pathway and the larger metabolism of amino acids. This enzyme works closely with ALDH4A1 which is another enzyme that catalyzes the earlier stages of the proline biosynthesis pathway before PYCR1 performs its function. The pathway plays a significant role in plant and animal cell adaptation to environmental changes. By modulating these pathways cells gain enhanced abilities to manage osmotic pressures and support vital functions under stress conditions linking PYCR1's activity to cellular survival and resilience.

PYCR1 mutations or dysfunction is associated with Cutis laxa a rare connective tissue disorder characterized by loose sagging skin and systemic involvement. These dysfunctions affect proline synthesis impairing collagen production which leads to abnormal connective tissue structure. PYCR1 is also connected to pyrroline-5-carboxylate synthetase implicating dysfunction in the larger metabolic framework that supports protein synthesis and cellular health. These disturbances underline the enzyme's importance in maintaining normal physiological conditions and hint at possible therapeutic targets for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
See full target information Pyrroline-5-carboxylate reductase 1, mitochondrial
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Discover. Oncology 13:50 PubMed35731336

2022

Circ_0000705 facilitates proline metabolism of esophageal squamous cell carcinoma cells by targeting miR-621/PYCR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cui-Juan Qian,Yi-Yang Tong,Lin-Ken Wu,Yi-Chao Wang,Xiao-Sheng Teng,Jun Yao

Journal of experimental & clinical cancer research : CR 41:45 PubMed35105345

2022

Pyrroline-5-Carboxylate Reductase 1: a novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Inge Oudaert,Hatice Satilmis,Philip Vlummens,Wouter De Brouwer,Anke Maes,Dirk Hose,Elke De Bruyne,Bart Ghesquière,Karin Vanderkerken,Kim De Veirman,Eline Menu
View all publications
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