AB226340

Anti-PYCR1 antibody - C-terminal

Name: Anti-PYCR1 antibody - C-terminal (ab226340) | Abcam
Brand: Abcam Limited
SKU: AB226340
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal PYCR1 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 250-350.

View Alternative Names

P5C reductase 1, P5CR 1, PYCR1

2 Images

Supplier Data

Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (AB226340)

PYCR1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226340 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226340 at 1 μg/ml.

Lane 1 : ab226340 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (ab226340)

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-PYCR1 antibody - C-terminal (AB226340)

Cell lysates were prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PYCR1 antibody - C-terminal (ab226340) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 33 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P32322

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab226340 was affinity purified using an epitope specific to PYCR1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCR1 also known as Pyrroline-5-carboxylate reductase 1 is an enzyme with a molecular weight of approximately 33 kDa. It facilitates the conversion of pyrroline-5-carboxylate to proline an essential amino acid. PYCR1 is mainly expressed in human tissues with high metabolic activity such as the liver kidney and pancreas which highlights its role in amino acid metabolism. This enzyme plays a central role in maintaining the balance between cellular oxidation and reduction and is typically active in the cytoplasm of the cells.

Biological function summary

PYCR1 participates in the proline biosynthesis pathway and contributes to the cellular response to stress. It functions as a homopolymer indicating its need to form complexes for activity. The production of proline is important as proline protects cells by maintaining membrane integrity and stabilizing proteins during environmental stress like oxidative stress. Proline synthesis involving PYCR1 is essential for collagen production which supports tissue and structural integrity in humans.

Pathways

PYCR1 acts within the proline biosynthesis pathway and the larger metabolism of amino acids. This enzyme works closely with ALDH4A1 which is another enzyme that catalyzes the earlier stages of the proline biosynthesis pathway before PYCR1 performs its function. The pathway plays a significant role in plant and animal cell adaptation to environmental changes. By modulating these pathways cells gain enhanced abilities to manage osmotic pressures and support vital functions under stress conditions linking PYCR1's activity to cellular survival and resilience.

PYCR1 mutations or dysfunction is associated with Cutis laxa a rare connective tissue disorder characterized by loose sagging skin and systemic involvement. These dysfunctions affect proline synthesis impairing collagen production which leads to abnormal connective tissue structure. PYCR1 is also connected to pyrroline-5-carboxylate synthetase implicating dysfunction in the larger metabolic framework that supports protein synthesis and cellular health. These disturbances underline the enzyme's importance in maintaining normal physiological conditions and hint at possible therapeutic targets for related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 16730026, PubMed : 19648921, PubMed : 23024808, PubMed : 28258219). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 16730026, PubMed : 23024808). Involved in the cellular response to oxidative stress (PubMed : 16730026, PubMed : 19648921).

See full target information Pyrroline-5-carboxylate reductase 1, mitochondrial

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's research & therapy 17:100 PubMed40336092

2025

Soluble SorLA in CSF, a novel biomarker to explore disrupted trafficking of SorLA protein in Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Castelot,Aline Zarea,David Wallon,Anne Rovelet-Lecrux,Catherine Schramm,Muriel Quillard-Muraine,Anne Beaume,Frédéric Blanc,Olivier Bousiges,Julien Dumurgier,Maïté Formaglio,Gwenael Leguyader,Sylvain Lehmann,Cecilia Marelli,Matthieu Martinet,Leonor Nogueira,Jérémie Pariente,Isabelle Quadrio,Adeline Rollin-Sillaire,Susanna Schraen,Gaël Nicolas,Magalie Lecourtois

The journal of gene medicine 25:e3527 PubMed37382425

2023

Antisense lncRNA-RP11-498C9.13 promotes bladder cancer progression by enhancing reactive oxygen species-induced mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Zhuo Li,Ke Yang,Zhiyong Gao,Qiang Zhou,Ping Li

Aging 12: PubMed33461172

2021

Dysregulation of USP18/FTO/PYCR1 signaling network promotes bladder cancer development and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Ke Yang,Jianjun Luo,Zhiyong Gao,Yunliang Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PYCR1 antibody - C-terminal

Immunoprecipitation - Anti-PYCR1 antibody - C-terminal (AB226340)

Western blot - Anti-PYCR1 antibody - C-terminal (AB226340)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise