AB279385

Anti-PYCR1 antibody [OTI4F2]

Name: Anti-PYCR1 antibody [OTI4F2] (ab279385) | Abcam
Brand: Abcam Limited
SKU: AB279385

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(7 Publications)

Mouse Monoclonal PYCR1 antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate - Human, Human, Mouse, Rat samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 1 to C-terminus.

View Alternative Names

P5C reductase 1, P5CR 1, PYCR1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Paraffin embedded human appendix tissue stained for PYCR1 using ab279385 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Paraffin embedded Human breast tissue stained for PYCR1 using ab279385 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Paraffin embedded human gastric carcinoma tissue stained for PYCR1 using ab279385 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Paraffin embedded adenocarcinoma of Human breast tissue stained for PYCR1 using ab279385 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Paraffin embedded carcinoma of human liver tissue stained for PYCR1 using ab279385 at 1/500 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.

Supplier Data

Western blot - Anti-PYCR1 antibody [OTI4F2] (AB279385)

All lanes:

Western blot - Anti-PYCR1 antibody [OTI4F2] (ab279385) at 1/500 dilution

Lane 1:

HepG2 cell extract at 35 µg

Lane 2:

HeLa cell extract at 35 µg

Lane 3:

HEK-293T cell extract at 35 µg

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4F2

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Pyrroline-5-carboxylate reductase 1, mitochondrial aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P32322

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCR1 also known as Pyrroline-5-carboxylate reductase 1 is an enzyme with a molecular weight of approximately 33 kDa. It facilitates the conversion of pyrroline-5-carboxylate to proline an essential amino acid. PYCR1 is mainly expressed in human tissues with high metabolic activity such as the liver kidney and pancreas which highlights its role in amino acid metabolism. This enzyme plays a central role in maintaining the balance between cellular oxidation and reduction and is typically active in the cytoplasm of the cells.

Biological function summary

PYCR1 participates in the proline biosynthesis pathway and contributes to the cellular response to stress. It functions as a homopolymer indicating its need to form complexes for activity. The production of proline is important as proline protects cells by maintaining membrane integrity and stabilizing proteins during environmental stress like oxidative stress. Proline synthesis involving PYCR1 is essential for collagen production which supports tissue and structural integrity in humans.

Pathways

PYCR1 acts within the proline biosynthesis pathway and the larger metabolism of amino acids. This enzyme works closely with ALDH4A1 which is another enzyme that catalyzes the earlier stages of the proline biosynthesis pathway before PYCR1 performs its function. The pathway plays a significant role in plant and animal cell adaptation to environmental changes. By modulating these pathways cells gain enhanced abilities to manage osmotic pressures and support vital functions under stress conditions linking PYCR1's activity to cellular survival and resilience.

PYCR1 mutations or dysfunction is associated with Cutis laxa a rare connective tissue disorder characterized by loose sagging skin and systemic involvement. These dysfunctions affect proline synthesis impairing collagen production which leads to abnormal connective tissue structure. PYCR1 is also connected to pyrroline-5-carboxylate synthetase implicating dysfunction in the larger metabolic framework that supports protein synthesis and cellular health. These disturbances underline the enzyme's importance in maintaining normal physiological conditions and hint at possible therapeutic targets for related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.

See full target information Pyrroline-5-carboxylate reductase 1, mitochondrial

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e35303 PubMed39170313

2024

ITPKA phosphorylates PYCR1 and promotes the progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangying Luo,Tao Chen,Junyi Deng,Ziyuan Liu,Changlong Bi,Song Lan

BMC cancer 24:682 PubMed38835015

2024

Astragaloside IV inhibits cell viability and glycolysis of hepatocellular carcinoma by regulating KAT2A-mediated succinylation of PGAM1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzhang Zhu,Fei Lu

Nature communications 15:3103 PubMed38600123

2024

Early-life exercise induces immunometabolic epigenetic modification enhancing anti-inflammatory immunity in middle-aged male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Nini Zhang,Xinpei Wang,Mengya Feng,Min Li,Jing Wang,Hongyan Yang,Siyu He,Ziqi Xia,Lei Shang,Xun Jiang,Mao Sun,Yuanming Wu,Chaoxue Ren,Xing Zhang,Jia Li,Feng Gao

Translational oncology 32:101667 PubMed37018868

2023

PYCR1 promotes the malignant progression of lung cancer through the JAK-STAT3 signaling pathway via PRODH-dependent glutamine synthesize.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Zhang,Xinyu Zhao,Enqin Wang,Ye Yang,Liangfeng Hu,Hongkun Xu,Baojun Zhang

Journal of clinical laboratory analysis 36:e24760 PubMed36371775

2022

LINC00511 aggravates the malignancy of lung adenocarcinoma through sponging microRNA miR-4739 to regulate pyrroline-5-carboxylate reductase 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Ye,Linxiu Wang,Yu Zeng

Current research in food science 5:1685-1700 PubMed36204709

2022

Potential role of gut microbiota-LCA-INSR axis in high fat-diet-induced non-alcoholic fatty liver dysfunction: From perspective of radiation variation.

Applications

Unspecified application

Species

Unspecified reactive species

Huiji Pan,Meiling Zhou,Zhao Ju,Jinhua Luo,Jing Jin,Liangfang Shen,Pingkun Zhou,Ruixue Huang

Communications biology 5:945 PubMed36088469

2022

Low-dose radiation exaggerates HFD-induced metabolic dysfunction by gut microbiota through PA-PYCR1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Ju,Peiyu Guo,Jing Xiang,Ridan Lei,Guofeng Ren,Meiling Zhou,Xiandan Yang,Pingkun Zhou,Ruixue Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PYCR1 antibody [OTI4F2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Western blot - Anti-PYCR1 antibody [OTI4F2] (AB279385)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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