AB103535

Anti-PYCR2 antibody

Name: Anti-PYCR2 antibody (ab103535) | Abcam
Brand: Abcam Limited
SKU: AB103535
Rating: 4 (3 reviews)

(3 Reviews)

(4 Publications)

Rabbit Polyclonal PYCR2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PYCR2.

View Alternative Names

Pyrroline-5-carboxylate reductase 2, P5C reductase 2, P5CR 2, PYCR2

4 Images

Lab

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot : Anti-PYCR2 antibody ab103535 staining at 1/500 dilution, shown in green; Mouse anti alpha Tubulin ab7291 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 34 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in PYCR2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

PYCR2 knockout MCF7 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Collaborator

Western blot - Anti-PYCR2 antibody (AB103535)

Blocking : 10% Milk

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 18 µg

Lane 2:

HeLa whole cell lysate at 36 µg

Lane 3:

RPE-1 whole cell lysate at 18 µg

Lane 4:

RPE-1 whole cell lysate at 36 µg

Secondary

All lanes:

LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 35 kDa

false

Exposure time: 7min

This image is courtesy of an anonymous abreview.

Unknown

Western blot - Anti-PYCR2 antibody (AB103535)

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 34 kDa,38 kDa

false

Unknown

Western blot - Anti-PYCR2 antibody (AB103535)

All lanes:

Western blot - Anti-PYCR2 antibody (ab103535) at 1/500 dilution

Lane 1:

PYCR2 transfected 293T cell line lysate at 25 µg

Lane 2:

Non-transfected 293T cell line lysate at 25 µg

Predicted band size: 20 kDa,34 kDa,39 kDa,81 kDa,92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PYCR2.

Q96C36

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCR2 also known as Pyrroline-5-carboxylate reductase 2 is an enzyme involved in proline biosynthesis. It has a molecular mass of approximately 31 kDa. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline an amino acid important for protein synthesis and cellular stress responses. Expressed broadly in human tissues PYCR2 shows notable expression in the liver brain and various muscle tissues indicating its wide functional relevance across different physiological systems.

Biological function summary

The enzyme participates in maintaining proline levels and contributes to cellular osmotic balance and protection against oxidative stress. It is not typically part of a larger protein complex but interacts with other metabolic enzymes that support similar stress response pathways. By catalyzing an important step in the proline biosynthesis pathway PYCR2 helps sustain cellular redox states and protein turnover rates under stressful conditions.

Pathways

PYCR2 plays a significant role in the proline metabolism pathway and glutamate pathway. The functioning of this enzyme in these pathways supports the synthesis and recycling of proline which is important for cell survival under osmotic and oxidative stress conditions. The enzyme cooperates indirectly with proteins such as glutamate dehydrogenase facilitating the metabolic flux through interconnected pathways that manage cellular nitrogen balance.

Mutations in PYCR2 have been associated with conditions such as Hypomyelinating Leukodystrophy 10 and Proline Metabolism Disorder. These conditions often showcase symptoms related to central nervous system abnormalities and metabolic imbalances. The disruptions in PYCR2 activity may also alter interactions with proteins involved in these pathways such as Δ1-Pyrroline-5-carboxylate synthase thereby exacerbating the disease states.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). Involved in cellular response to oxidative stress (PubMed : 25865492). In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+) (PubMed : 2722838, PubMed : 6894153).

See full target information PYCR2

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 41:45 PubMed35105345

2022

Pyrroline-5-Carboxylate Reductase 1: a novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Inge Oudaert,Hatice Satilmis,Philip Vlummens,Wouter De Brouwer,Anke Maes,Dirk Hose,Elke De Bruyne,Bart Ghesquière,Karin Vanderkerken,Kim De Veirman,Eline Menu

Disease markers 2021:9950663 PubMed34512817

2021

Pyrroline-5-Carboxylate Reductase-2 Promotes Colorectal Cancer Progression via Activating PI3K/AKT/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Yin,Xiaoxia Huang,Yi Xuan

Oncology letters 19:3815-3826 PubMed32391095

2020

Identification of potential cervical cancer serum biomarkers in Thai patients.

Applications

Unspecified application

Species

Unspecified reactive species

Siriporn Keeratichamroen,Pantipa Subhasitanont,Daranee Chokchaichamnankit,Churat Weeraphan,Kittirat Saharat,Narongrit Sritana,Nuttavut Kantathavorn,Kriangpol Wiriyaukaradecha,Thaniya Sricharunrat,N Monique Paricharttanakul,Chirayu Auewarakul,Jisnuson Svasti,Chantragan Srisomsap

Regenerative therapy 6:83-89 PubMed30271842

2017

Cytological character of mini pig mesenchymal stromal cells from various tissues and the attempt of cell sheet formation.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuyuki Kaibuchi,Takanori Iwata,Satoru Onizuka,Kosei Yano,Masayuki Yamato,Teruo Okano,Tomohiro Ando

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PYCR2 antibody

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot - Anti-PYCR2 antibody (AB103535)

Western blot - Anti-PYCR2 antibody (AB103535)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise